ym155 Search Results


ym155  (MedChemExpress)
94
MedChemExpress ym155
PAB-induced ferroptosis depends on upregulation of Survivin. ( A ) Western blotting analysis is conducted on the lysates of A549 and H460 cells treated with PAB (10 µM) for 48 h. ( B ) Western blotting analysis is conducted BIRC5 -knockdown A549 and H460 cells. ( C ) Crystal violet staining determines the viability of A549 and H460 cells after 72 h of treatment with PAB (10 µM) in the presence or absence of <t>YM155</t> (10 µM). ( D ) The viability of A549 and H460 cells treated with PAB (10 µM) for 72 h in the presence or absence of YM155 (10 µM) is determined by CCK8 assay. ( E ) Analysis of cell viability in control and BIRC5 -knockdown A549 and H460 cells following treatment with PAB (10 µM) for 72 h by CCK8 assay. ( F ) The quantitative analysis of cell apoptosis of A549 and H460 cells treated with PAB (10 µM) for 72 h in the presence or absence of YM155 (10 µM) is determined. ( G ) The quantitative analysis of cell apoptosis in control and BIRC5 -knockdown A549 and H460 cells following treatment with PAB (10 µM) for 72 h. ( H ) The intracellular Fe 2+ in A549 and H460 cells are assessed after treatment with PAB (10 µM) either in the presence or absence of YM155 (10 µM) for 24 h, followed by extraction for absorbance measurement at 550 nm. ( I ) Lipid peroxidation products are observed and statistically analyzed for 24 h after PAB stimulation of A549 and H460 cells in the presence or absence of YM155 (10 µM). The red color indicated non-oxidized form and green color indicated oxidized form. Scale bar, 20 μm. ( J ) Western blotting analysis is performed on A549 and H460 cell lysates treated with PAB (10 µM) for 48 h in the presence or absence of YM155 (10 µM). ( K ) Western blotting analysis is performed and BIRC5 -knockdown A549 and H460 cells following treatment with PAB (10 µM) for 48 h. The results are presented as the means ± S .D., n ≥ 3, * p < 0.05, ** p < 0.01, *** p < 0.001 versus control group.
Ym155, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 1 article reviews
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ym155  (TargetMol)
93
TargetMol ym155
PAB-induced ferroptosis depends on upregulation of Survivin. ( A ) Western blotting analysis is conducted on the lysates of A549 and H460 cells treated with PAB (10 µM) for 48 h. ( B ) Western blotting analysis is conducted BIRC5 -knockdown A549 and H460 cells. ( C ) Crystal violet staining determines the viability of A549 and H460 cells after 72 h of treatment with PAB (10 µM) in the presence or absence of <t>YM155</t> (10 µM). ( D ) The viability of A549 and H460 cells treated with PAB (10 µM) for 72 h in the presence or absence of YM155 (10 µM) is determined by CCK8 assay. ( E ) Analysis of cell viability in control and BIRC5 -knockdown A549 and H460 cells following treatment with PAB (10 µM) for 72 h by CCK8 assay. ( F ) The quantitative analysis of cell apoptosis of A549 and H460 cells treated with PAB (10 µM) for 72 h in the presence or absence of YM155 (10 µM) is determined. ( G ) The quantitative analysis of cell apoptosis in control and BIRC5 -knockdown A549 and H460 cells following treatment with PAB (10 µM) for 72 h. ( H ) The intracellular Fe 2+ in A549 and H460 cells are assessed after treatment with PAB (10 µM) either in the presence or absence of YM155 (10 µM) for 24 h, followed by extraction for absorbance measurement at 550 nm. ( I ) Lipid peroxidation products are observed and statistically analyzed for 24 h after PAB stimulation of A549 and H460 cells in the presence or absence of YM155 (10 µM). The red color indicated non-oxidized form and green color indicated oxidized form. Scale bar, 20 μm. ( J ) Western blotting analysis is performed on A549 and H460 cell lysates treated with PAB (10 µM) for 48 h in the presence or absence of YM155 (10 µM). ( K ) Western blotting analysis is performed and BIRC5 -knockdown A549 and H460 cells following treatment with PAB (10 µM) for 48 h. The results are presented as the means ± S .D., n ≥ 3, * p < 0.05, ** p < 0.01, *** p < 0.001 versus control group.
Ym155, supplied by TargetMol, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ym155  (Santa Cruz Biotechnology)
90
Santa Cruz Biotechnology ym155
PAB-induced ferroptosis depends on upregulation of Survivin. ( A ) Western blotting analysis is conducted on the lysates of A549 and H460 cells treated with PAB (10 µM) for 48 h. ( B ) Western blotting analysis is conducted BIRC5 -knockdown A549 and H460 cells. ( C ) Crystal violet staining determines the viability of A549 and H460 cells after 72 h of treatment with PAB (10 µM) in the presence or absence of <t>YM155</t> (10 µM). ( D ) The viability of A549 and H460 cells treated with PAB (10 µM) for 72 h in the presence or absence of YM155 (10 µM) is determined by CCK8 assay. ( E ) Analysis of cell viability in control and BIRC5 -knockdown A549 and H460 cells following treatment with PAB (10 µM) for 72 h by CCK8 assay. ( F ) The quantitative analysis of cell apoptosis of A549 and H460 cells treated with PAB (10 µM) for 72 h in the presence or absence of YM155 (10 µM) is determined. ( G ) The quantitative analysis of cell apoptosis in control and BIRC5 -knockdown A549 and H460 cells following treatment with PAB (10 µM) for 72 h. ( H ) The intracellular Fe 2+ in A549 and H460 cells are assessed after treatment with PAB (10 µM) either in the presence or absence of YM155 (10 µM) for 24 h, followed by extraction for absorbance measurement at 550 nm. ( I ) Lipid peroxidation products are observed and statistically analyzed for 24 h after PAB stimulation of A549 and H460 cells in the presence or absence of YM155 (10 µM). The red color indicated non-oxidized form and green color indicated oxidized form. Scale bar, 20 μm. ( J ) Western blotting analysis is performed on A549 and H460 cell lysates treated with PAB (10 µM) for 48 h in the presence or absence of YM155 (10 µM). ( K ) Western blotting analysis is performed and BIRC5 -knockdown A549 and H460 cells following treatment with PAB (10 µM) for 48 h. The results are presented as the means ± S .D., n ≥ 3, * p < 0.05, ** p < 0.01, *** p < 0.001 versus control group.
Ym155, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ym155  (Selleck Chemicals)
94
Selleck Chemicals ym155
PAB-induced ferroptosis depends on upregulation of Survivin. ( A ) Western blotting analysis is conducted on the lysates of A549 and H460 cells treated with PAB (10 µM) for 48 h. ( B ) Western blotting analysis is conducted BIRC5 -knockdown A549 and H460 cells. ( C ) Crystal violet staining determines the viability of A549 and H460 cells after 72 h of treatment with PAB (10 µM) in the presence or absence of <t>YM155</t> (10 µM). ( D ) The viability of A549 and H460 cells treated with PAB (10 µM) for 72 h in the presence or absence of YM155 (10 µM) is determined by CCK8 assay. ( E ) Analysis of cell viability in control and BIRC5 -knockdown A549 and H460 cells following treatment with PAB (10 µM) for 72 h by CCK8 assay. ( F ) The quantitative analysis of cell apoptosis of A549 and H460 cells treated with PAB (10 µM) for 72 h in the presence or absence of YM155 (10 µM) is determined. ( G ) The quantitative analysis of cell apoptosis in control and BIRC5 -knockdown A549 and H460 cells following treatment with PAB (10 µM) for 72 h. ( H ) The intracellular Fe 2+ in A549 and H460 cells are assessed after treatment with PAB (10 µM) either in the presence or absence of YM155 (10 µM) for 24 h, followed by extraction for absorbance measurement at 550 nm. ( I ) Lipid peroxidation products are observed and statistically analyzed for 24 h after PAB stimulation of A549 and H460 cells in the presence or absence of YM155 (10 µM). The red color indicated non-oxidized form and green color indicated oxidized form. Scale bar, 20 μm. ( J ) Western blotting analysis is performed on A549 and H460 cell lysates treated with PAB (10 µM) for 48 h in the presence or absence of YM155 (10 µM). ( K ) Western blotting analysis is performed and BIRC5 -knockdown A549 and H460 cells following treatment with PAB (10 µM) for 48 h. The results are presented as the means ± S .D., n ≥ 3, * p < 0.05, ** p < 0.01, *** p < 0.001 versus control group.
Ym155, supplied by Selleck Chemicals, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 1 article reviews
ym155 - by Bioz Stars, 2026-04
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ym155  (Tocris)
93
Tocris ym155
PAB-induced ferroptosis depends on upregulation of Survivin. ( A ) Western blotting analysis is conducted on the lysates of A549 and H460 cells treated with PAB (10 µM) for 48 h. ( B ) Western blotting analysis is conducted BIRC5 -knockdown A549 and H460 cells. ( C ) Crystal violet staining determines the viability of A549 and H460 cells after 72 h of treatment with PAB (10 µM) in the presence or absence of <t>YM155</t> (10 µM). ( D ) The viability of A549 and H460 cells treated with PAB (10 µM) for 72 h in the presence or absence of YM155 (10 µM) is determined by CCK8 assay. ( E ) Analysis of cell viability in control and BIRC5 -knockdown A549 and H460 cells following treatment with PAB (10 µM) for 72 h by CCK8 assay. ( F ) The quantitative analysis of cell apoptosis of A549 and H460 cells treated with PAB (10 µM) for 72 h in the presence or absence of YM155 (10 µM) is determined. ( G ) The quantitative analysis of cell apoptosis in control and BIRC5 -knockdown A549 and H460 cells following treatment with PAB (10 µM) for 72 h. ( H ) The intracellular Fe 2+ in A549 and H460 cells are assessed after treatment with PAB (10 µM) either in the presence or absence of YM155 (10 µM) for 24 h, followed by extraction for absorbance measurement at 550 nm. ( I ) Lipid peroxidation products are observed and statistically analyzed for 24 h after PAB stimulation of A549 and H460 cells in the presence or absence of YM155 (10 µM). The red color indicated non-oxidized form and green color indicated oxidized form. Scale bar, 20 μm. ( J ) Western blotting analysis is performed on A549 and H460 cell lysates treated with PAB (10 µM) for 48 h in the presence or absence of YM155 (10 µM). ( K ) Western blotting analysis is performed and BIRC5 -knockdown A549 and H460 cells following treatment with PAB (10 µM) for 48 h. The results are presented as the means ± S .D., n ≥ 3, * p < 0.05, ** p < 0.01, *** p < 0.001 versus control group.
Ym155, supplied by Tocris, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ym155  (Astellas)
90
Astellas ym155
PAB-induced ferroptosis depends on upregulation of Survivin. ( A ) Western blotting analysis is conducted on the lysates of A549 and H460 cells treated with PAB (10 µM) for 48 h. ( B ) Western blotting analysis is conducted BIRC5 -knockdown A549 and H460 cells. ( C ) Crystal violet staining determines the viability of A549 and H460 cells after 72 h of treatment with PAB (10 µM) in the presence or absence of <t>YM155</t> (10 µM). ( D ) The viability of A549 and H460 cells treated with PAB (10 µM) for 72 h in the presence or absence of YM155 (10 µM) is determined by CCK8 assay. ( E ) Analysis of cell viability in control and BIRC5 -knockdown A549 and H460 cells following treatment with PAB (10 µM) for 72 h by CCK8 assay. ( F ) The quantitative analysis of cell apoptosis of A549 and H460 cells treated with PAB (10 µM) for 72 h in the presence or absence of YM155 (10 µM) is determined. ( G ) The quantitative analysis of cell apoptosis in control and BIRC5 -knockdown A549 and H460 cells following treatment with PAB (10 µM) for 72 h. ( H ) The intracellular Fe 2+ in A549 and H460 cells are assessed after treatment with PAB (10 µM) either in the presence or absence of YM155 (10 µM) for 24 h, followed by extraction for absorbance measurement at 550 nm. ( I ) Lipid peroxidation products are observed and statistically analyzed for 24 h after PAB stimulation of A549 and H460 cells in the presence or absence of YM155 (10 µM). The red color indicated non-oxidized form and green color indicated oxidized form. Scale bar, 20 μm. ( J ) Western blotting analysis is performed on A549 and H460 cell lysates treated with PAB (10 µM) for 48 h in the presence or absence of YM155 (10 µM). ( K ) Western blotting analysis is performed and BIRC5 -knockdown A549 and H460 cells following treatment with PAB (10 µM) for 48 h. The results are presented as the means ± S .D., n ≥ 3, * p < 0.05, ** p < 0.01, *** p < 0.001 versus control group.
Ym155, supplied by Astellas, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ym155  (Cayman Chemical)
90
Cayman Chemical ym155
PAB-induced ferroptosis depends on upregulation of Survivin. ( A ) Western blotting analysis is conducted on the lysates of A549 and H460 cells treated with PAB (10 µM) for 48 h. ( B ) Western blotting analysis is conducted BIRC5 -knockdown A549 and H460 cells. ( C ) Crystal violet staining determines the viability of A549 and H460 cells after 72 h of treatment with PAB (10 µM) in the presence or absence of <t>YM155</t> (10 µM). ( D ) The viability of A549 and H460 cells treated with PAB (10 µM) for 72 h in the presence or absence of YM155 (10 µM) is determined by CCK8 assay. ( E ) Analysis of cell viability in control and BIRC5 -knockdown A549 and H460 cells following treatment with PAB (10 µM) for 72 h by CCK8 assay. ( F ) The quantitative analysis of cell apoptosis of A549 and H460 cells treated with PAB (10 µM) for 72 h in the presence or absence of YM155 (10 µM) is determined. ( G ) The quantitative analysis of cell apoptosis in control and BIRC5 -knockdown A549 and H460 cells following treatment with PAB (10 µM) for 72 h. ( H ) The intracellular Fe 2+ in A549 and H460 cells are assessed after treatment with PAB (10 µM) either in the presence or absence of YM155 (10 µM) for 24 h, followed by extraction for absorbance measurement at 550 nm. ( I ) Lipid peroxidation products are observed and statistically analyzed for 24 h after PAB stimulation of A549 and H460 cells in the presence or absence of YM155 (10 µM). The red color indicated non-oxidized form and green color indicated oxidized form. Scale bar, 20 μm. ( J ) Western blotting analysis is performed on A549 and H460 cell lysates treated with PAB (10 µM) for 48 h in the presence or absence of YM155 (10 µM). ( K ) Western blotting analysis is performed and BIRC5 -knockdown A549 and H460 cells following treatment with PAB (10 µM) for 48 h. The results are presented as the means ± S .D., n ≥ 3, * p < 0.05, ** p < 0.01, *** p < 0.001 versus control group.
Ym155, supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ym155  (Adooq Bioscience LLC)
90
Adooq Bioscience LLC ym155
PAB-induced ferroptosis depends on upregulation of Survivin. ( A ) Western blotting analysis is conducted on the lysates of A549 and H460 cells treated with PAB (10 µM) for 48 h. ( B ) Western blotting analysis is conducted BIRC5 -knockdown A549 and H460 cells. ( C ) Crystal violet staining determines the viability of A549 and H460 cells after 72 h of treatment with PAB (10 µM) in the presence or absence of <t>YM155</t> (10 µM). ( D ) The viability of A549 and H460 cells treated with PAB (10 µM) for 72 h in the presence or absence of YM155 (10 µM) is determined by CCK8 assay. ( E ) Analysis of cell viability in control and BIRC5 -knockdown A549 and H460 cells following treatment with PAB (10 µM) for 72 h by CCK8 assay. ( F ) The quantitative analysis of cell apoptosis of A549 and H460 cells treated with PAB (10 µM) for 72 h in the presence or absence of YM155 (10 µM) is determined. ( G ) The quantitative analysis of cell apoptosis in control and BIRC5 -knockdown A549 and H460 cells following treatment with PAB (10 µM) for 72 h. ( H ) The intracellular Fe 2+ in A549 and H460 cells are assessed after treatment with PAB (10 µM) either in the presence or absence of YM155 (10 µM) for 24 h, followed by extraction for absorbance measurement at 550 nm. ( I ) Lipid peroxidation products are observed and statistically analyzed for 24 h after PAB stimulation of A549 and H460 cells in the presence or absence of YM155 (10 µM). The red color indicated non-oxidized form and green color indicated oxidized form. Scale bar, 20 μm. ( J ) Western blotting analysis is performed on A549 and H460 cell lysates treated with PAB (10 µM) for 48 h in the presence or absence of YM155 (10 µM). ( K ) Western blotting analysis is performed and BIRC5 -knockdown A549 and H460 cells following treatment with PAB (10 µM) for 48 h. The results are presented as the means ± S .D., n ≥ 3, * p < 0.05, ** p < 0.01, *** p < 0.001 versus control group.
Ym155, supplied by Adooq Bioscience LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ym155  (ChemScene llc)
90
ChemScene llc ym155
PAB-induced ferroptosis depends on upregulation of Survivin. ( A ) Western blotting analysis is conducted on the lysates of A549 and H460 cells treated with PAB (10 µM) for 48 h. ( B ) Western blotting analysis is conducted BIRC5 -knockdown A549 and H460 cells. ( C ) Crystal violet staining determines the viability of A549 and H460 cells after 72 h of treatment with PAB (10 µM) in the presence or absence of <t>YM155</t> (10 µM). ( D ) The viability of A549 and H460 cells treated with PAB (10 µM) for 72 h in the presence or absence of YM155 (10 µM) is determined by CCK8 assay. ( E ) Analysis of cell viability in control and BIRC5 -knockdown A549 and H460 cells following treatment with PAB (10 µM) for 72 h by CCK8 assay. ( F ) The quantitative analysis of cell apoptosis of A549 and H460 cells treated with PAB (10 µM) for 72 h in the presence or absence of YM155 (10 µM) is determined. ( G ) The quantitative analysis of cell apoptosis in control and BIRC5 -knockdown A549 and H460 cells following treatment with PAB (10 µM) for 72 h. ( H ) The intracellular Fe 2+ in A549 and H460 cells are assessed after treatment with PAB (10 µM) either in the presence or absence of YM155 (10 µM) for 24 h, followed by extraction for absorbance measurement at 550 nm. ( I ) Lipid peroxidation products are observed and statistically analyzed for 24 h after PAB stimulation of A549 and H460 cells in the presence or absence of YM155 (10 µM). The red color indicated non-oxidized form and green color indicated oxidized form. Scale bar, 20 μm. ( J ) Western blotting analysis is performed on A549 and H460 cell lysates treated with PAB (10 µM) for 48 h in the presence or absence of YM155 (10 µM). ( K ) Western blotting analysis is performed and BIRC5 -knockdown A549 and H460 cells following treatment with PAB (10 µM) for 48 h. The results are presented as the means ± S .D., n ≥ 3, * p < 0.05, ** p < 0.01, *** p < 0.001 versus control group.
Ym155, supplied by ChemScene llc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ym155 a4221  (ApexBio)
90
ApexBio ym155 a4221
PAB-induced ferroptosis depends on upregulation of Survivin. ( A ) Western blotting analysis is conducted on the lysates of A549 and H460 cells treated with PAB (10 µM) for 48 h. ( B ) Western blotting analysis is conducted BIRC5 -knockdown A549 and H460 cells. ( C ) Crystal violet staining determines the viability of A549 and H460 cells after 72 h of treatment with PAB (10 µM) in the presence or absence of <t>YM155</t> (10 µM). ( D ) The viability of A549 and H460 cells treated with PAB (10 µM) for 72 h in the presence or absence of YM155 (10 µM) is determined by CCK8 assay. ( E ) Analysis of cell viability in control and BIRC5 -knockdown A549 and H460 cells following treatment with PAB (10 µM) for 72 h by CCK8 assay. ( F ) The quantitative analysis of cell apoptosis of A549 and H460 cells treated with PAB (10 µM) for 72 h in the presence or absence of YM155 (10 µM) is determined. ( G ) The quantitative analysis of cell apoptosis in control and BIRC5 -knockdown A549 and H460 cells following treatment with PAB (10 µM) for 72 h. ( H ) The intracellular Fe 2+ in A549 and H460 cells are assessed after treatment with PAB (10 µM) either in the presence or absence of YM155 (10 µM) for 24 h, followed by extraction for absorbance measurement at 550 nm. ( I ) Lipid peroxidation products are observed and statistically analyzed for 24 h after PAB stimulation of A549 and H460 cells in the presence or absence of YM155 (10 µM). The red color indicated non-oxidized form and green color indicated oxidized form. Scale bar, 20 μm. ( J ) Western blotting analysis is performed on A549 and H460 cell lysates treated with PAB (10 µM) for 48 h in the presence or absence of YM155 (10 µM). ( K ) Western blotting analysis is performed and BIRC5 -knockdown A549 and H460 cells following treatment with PAB (10 µM) for 48 h. The results are presented as the means ± S .D., n ≥ 3, * p < 0.05, ** p < 0.01, *** p < 0.001 versus control group.
Ym155 A4221, supplied by ApexBio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ym155  (Changchun Jilin University Little Swan)
90
Changchun Jilin University Little Swan ym155
PAB-induced ferroptosis depends on upregulation of Survivin. ( A ) Western blotting analysis is conducted on the lysates of A549 and H460 cells treated with PAB (10 µM) for 48 h. ( B ) Western blotting analysis is conducted BIRC5 -knockdown A549 and H460 cells. ( C ) Crystal violet staining determines the viability of A549 and H460 cells after 72 h of treatment with PAB (10 µM) in the presence or absence of <t>YM155</t> (10 µM). ( D ) The viability of A549 and H460 cells treated with PAB (10 µM) for 72 h in the presence or absence of YM155 (10 µM) is determined by CCK8 assay. ( E ) Analysis of cell viability in control and BIRC5 -knockdown A549 and H460 cells following treatment with PAB (10 µM) for 72 h by CCK8 assay. ( F ) The quantitative analysis of cell apoptosis of A549 and H460 cells treated with PAB (10 µM) for 72 h in the presence or absence of YM155 (10 µM) is determined. ( G ) The quantitative analysis of cell apoptosis in control and BIRC5 -knockdown A549 and H460 cells following treatment with PAB (10 µM) for 72 h. ( H ) The intracellular Fe 2+ in A549 and H460 cells are assessed after treatment with PAB (10 µM) either in the presence or absence of YM155 (10 µM) for 24 h, followed by extraction for absorbance measurement at 550 nm. ( I ) Lipid peroxidation products are observed and statistically analyzed for 24 h after PAB stimulation of A549 and H460 cells in the presence or absence of YM155 (10 µM). The red color indicated non-oxidized form and green color indicated oxidized form. Scale bar, 20 μm. ( J ) Western blotting analysis is performed on A549 and H460 cell lysates treated with PAB (10 µM) for 48 h in the presence or absence of YM155 (10 µM). ( K ) Western blotting analysis is performed and BIRC5 -knockdown A549 and H460 cells following treatment with PAB (10 µM) for 48 h. The results are presented as the means ± S .D., n ≥ 3, * p < 0.05, ** p < 0.01, *** p < 0.001 versus control group.
Ym155, supplied by Changchun Jilin University Little Swan, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


PAB-induced ferroptosis depends on upregulation of Survivin. ( A ) Western blotting analysis is conducted on the lysates of A549 and H460 cells treated with PAB (10 µM) for 48 h. ( B ) Western blotting analysis is conducted BIRC5 -knockdown A549 and H460 cells. ( C ) Crystal violet staining determines the viability of A549 and H460 cells after 72 h of treatment with PAB (10 µM) in the presence or absence of YM155 (10 µM). ( D ) The viability of A549 and H460 cells treated with PAB (10 µM) for 72 h in the presence or absence of YM155 (10 µM) is determined by CCK8 assay. ( E ) Analysis of cell viability in control and BIRC5 -knockdown A549 and H460 cells following treatment with PAB (10 µM) for 72 h by CCK8 assay. ( F ) The quantitative analysis of cell apoptosis of A549 and H460 cells treated with PAB (10 µM) for 72 h in the presence or absence of YM155 (10 µM) is determined. ( G ) The quantitative analysis of cell apoptosis in control and BIRC5 -knockdown A549 and H460 cells following treatment with PAB (10 µM) for 72 h. ( H ) The intracellular Fe 2+ in A549 and H460 cells are assessed after treatment with PAB (10 µM) either in the presence or absence of YM155 (10 µM) for 24 h, followed by extraction for absorbance measurement at 550 nm. ( I ) Lipid peroxidation products are observed and statistically analyzed for 24 h after PAB stimulation of A549 and H460 cells in the presence or absence of YM155 (10 µM). The red color indicated non-oxidized form and green color indicated oxidized form. Scale bar, 20 μm. ( J ) Western blotting analysis is performed on A549 and H460 cell lysates treated with PAB (10 µM) for 48 h in the presence or absence of YM155 (10 µM). ( K ) Western blotting analysis is performed and BIRC5 -knockdown A549 and H460 cells following treatment with PAB (10 µM) for 48 h. The results are presented as the means ± S .D., n ≥ 3, * p < 0.05, ** p < 0.01, *** p < 0.001 versus control group.

Journal: Scientific Reports

Article Title: Pseudolaric acid B promotes lung cancer cells ferroptosis depending on JNK/ERK-mediated upregulation of survivin

doi: 10.1038/s41598-026-36423-3

Figure Lengend Snippet: PAB-induced ferroptosis depends on upregulation of Survivin. ( A ) Western blotting analysis is conducted on the lysates of A549 and H460 cells treated with PAB (10 µM) for 48 h. ( B ) Western blotting analysis is conducted BIRC5 -knockdown A549 and H460 cells. ( C ) Crystal violet staining determines the viability of A549 and H460 cells after 72 h of treatment with PAB (10 µM) in the presence or absence of YM155 (10 µM). ( D ) The viability of A549 and H460 cells treated with PAB (10 µM) for 72 h in the presence or absence of YM155 (10 µM) is determined by CCK8 assay. ( E ) Analysis of cell viability in control and BIRC5 -knockdown A549 and H460 cells following treatment with PAB (10 µM) for 72 h by CCK8 assay. ( F ) The quantitative analysis of cell apoptosis of A549 and H460 cells treated with PAB (10 µM) for 72 h in the presence or absence of YM155 (10 µM) is determined. ( G ) The quantitative analysis of cell apoptosis in control and BIRC5 -knockdown A549 and H460 cells following treatment with PAB (10 µM) for 72 h. ( H ) The intracellular Fe 2+ in A549 and H460 cells are assessed after treatment with PAB (10 µM) either in the presence or absence of YM155 (10 µM) for 24 h, followed by extraction for absorbance measurement at 550 nm. ( I ) Lipid peroxidation products are observed and statistically analyzed for 24 h after PAB stimulation of A549 and H460 cells in the presence or absence of YM155 (10 µM). The red color indicated non-oxidized form and green color indicated oxidized form. Scale bar, 20 μm. ( J ) Western blotting analysis is performed on A549 and H460 cell lysates treated with PAB (10 µM) for 48 h in the presence or absence of YM155 (10 µM). ( K ) Western blotting analysis is performed and BIRC5 -knockdown A549 and H460 cells following treatment with PAB (10 µM) for 48 h. The results are presented as the means ± S .D., n ≥ 3, * p < 0.05, ** p < 0.01, *** p < 0.001 versus control group.

Article Snippet: PAB (HY-N6939), DFO (HY-B0568), SP600125 (HY-12041), U0126 (HY-12031 A), YM155 (HY-10194), z-VAD-FMK (HY-16658B), CQ (HY-17589 A) were purchased from MedChem Express (Shanghai, China).

Techniques: Western Blot, Knockdown, Staining, CCK-8 Assay, Control, Extraction