yap1 nb110 58358 Search Results


95
Novus Biologicals rabbit anti yap
Rabbit Anti Yap, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/yap1+nb110+58358/YAP1+Antibody+-+BSA+Free/10__1681_slash_asn__2014090916-133-9-11
Average 95 stars, based on 1 article reviews
rabbit anti yap - by Bioz Stars, 2026-10
95/100 stars
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95
Novus Biologicals antibodies against yap1
A, Scoring of tissue microarrays containing 78 pancreatic ductal adenocarcinoma samples immunohistochemically stained for YAP or UGP2. +, ++, +++ represent combined scoring of automated area x intensity measurements. **** p < 0.0001 by Chi-square test. B, qPCR for UGP2 messenger RNA in Panc1, MiaPaca2, and Suit2 cells with shYAP or empty vector. *** p < 0.001. C, Immunoblot on lysates from Panc1, MiaPaca2, and Suit2 cells stably expressing shYAP or empty vector and probed as indicated. D-E, Immunoblots on lysates from MCF10A (D) and 293T (E) cells stably expressing wild-type YAP, YAP5SA, YAP S94A, or empty vector, probed as indicated. F, ChIP-seq for <t>YAP1</t> and TEAD4 at the UGP2 locus. G, Targeted PCR using two sets of primers (p1 and p2) in the UGP2 promoter region of Panc1 cells after crosslinking and pulldown using antibodies against either YAP, TEAD4, or control IgG. Fold enrichment is percent input target gene/percent input Actin. * p < 0.05, ** p < 0.01.
Antibodies Against Yap1, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/yap1+nb110+58358/YAP1+Antibody+-+BSA+Free/bio_rxiv__2020__10__13__337998-137-9-12
Average 95 stars, based on 1 article reviews
antibodies against yap1 - by Bioz Stars, 2026-10
95/100 stars
  Buy from Supplier

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A, Scoring of tissue microarrays containing 78 pancreatic ductal adenocarcinoma samples immunohistochemically stained for YAP or UGP2. +, ++, +++ represent combined scoring of automated area x intensity measurements. **** p < 0.0001 by Chi-square test. B, qPCR for UGP2 messenger RNA in Panc1, MiaPaca2, and Suit2 cells with shYAP or empty vector. *** p < 0.001. C, Immunoblot on lysates from Panc1, MiaPaca2, and Suit2 cells stably expressing shYAP or empty vector and probed as indicated. D-E, Immunoblots on lysates from MCF10A (D) and 293T (E) cells stably expressing wild-type YAP, YAP5SA, YAP S94A, or empty vector, probed as indicated. F, ChIP-seq for YAP1 and TEAD4 at the UGP2 locus. G, Targeted PCR using two sets of primers (p1 and p2) in the UGP2 promoter region of Panc1 cells after crosslinking and pulldown using antibodies against either YAP, TEAD4, or control IgG. Fold enrichment is percent input target gene/percent input Actin. * p < 0.05, ** p < 0.01.

Journal: bioRxiv

Article Title: UDP-glucose pyrophosphorylase 2, a regulator of glycosylation and glycogen, is essential for pancreatic cancer growth

doi: 10.1101/2020.10.13.337998

Figure Lengend Snippet: A, Scoring of tissue microarrays containing 78 pancreatic ductal adenocarcinoma samples immunohistochemically stained for YAP or UGP2. +, ++, +++ represent combined scoring of automated area x intensity measurements. **** p < 0.0001 by Chi-square test. B, qPCR for UGP2 messenger RNA in Panc1, MiaPaca2, and Suit2 cells with shYAP or empty vector. *** p < 0.001. C, Immunoblot on lysates from Panc1, MiaPaca2, and Suit2 cells stably expressing shYAP or empty vector and probed as indicated. D-E, Immunoblots on lysates from MCF10A (D) and 293T (E) cells stably expressing wild-type YAP, YAP5SA, YAP S94A, or empty vector, probed as indicated. F, ChIP-seq for YAP1 and TEAD4 at the UGP2 locus. G, Targeted PCR using two sets of primers (p1 and p2) in the UGP2 promoter region of Panc1 cells after crosslinking and pulldown using antibodies against either YAP, TEAD4, or control IgG. Fold enrichment is percent input target gene/percent input Actin. * p < 0.05, ** p < 0.01.

Article Snippet: Global ChIP-seq and UGP2 promoter ChIP-PCR were performed using antibodies against YAP1 (Novus Biologicals NB110-58358) and TEAD4 (ab58310).

Techniques: Staining, Plasmid Preparation, Western Blot, Stable Transfection, Expressing, ChIP-sequencing, Control