y-39983 Search Results


y-39983 dihydrochloride  (MuseChem Chemicals)
N/A
Y-33075 dihydrochloride (Cat No.:I002265) is a chemical compound that acts as a selective inhibitor of the rho-associated protein kinase (ROCK). ROCK is an enzyme involved in the regulation of cell contraction and migration. By inhibiting
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y 33075  (MedChemExpress)
93
MedChemExpress y 33075
(A) Schematic depiction of the RhoA-ROCK pathway including mechanism of action of the ROCK inhibitors Y-27632 and <t>Y-33075.</t> (B) Chemical structure of ROCK inhibitors Y-27632 and Y33075. (C) Effect of Y-27632 and Y-33075 on collagen gel contraction of activated human TWNT-4 cells (n = 3). (D) Representative images of the contraction assay in TWNT-4 cells after incubation with the ROCK inhibitors for 24 hours. (E) Effect of Y-27632 and Y-33075 on collagen gel contraction of culture-activated primary murine FVB/NJ cells (n = 3). (F) Representative images of gel contraction in FVB/NJ cells 24 hours after treatment with the ROCK inhibitors. (G) Protein expression levels of phospho-MLC and MLC in TWNT-4 after 24 hour incubation with the ROCK inhibitors. (H) Ratio of phospho-MLC to MLC in TWNT-4 cell lysates after treatment with ROCK inhibitors for 24 hours (n = 2). Results are expressed as the mean ± standard error of the mean (SEM); *p<0.05, **p<0.01 and ***p<0.001.
Y 33075, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/y-39983/Y-33075/pmc09888688-39-9-10
Average 93 stars, based on 1 article reviews
y 33075 - by Bioz Stars, 2026-10
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y 39983 hcl  (Selleck Chemicals)
92
Selleck Chemicals y 39983 hcl
(A) Schematic depiction of the RhoA-ROCK pathway including mechanism of action of the ROCK inhibitors Y-27632 and <t>Y-33075.</t> (B) Chemical structure of ROCK inhibitors Y-27632 and Y33075. (C) Effect of Y-27632 and Y-33075 on collagen gel contraction of activated human TWNT-4 cells (n = 3). (D) Representative images of the contraction assay in TWNT-4 cells after incubation with the ROCK inhibitors for 24 hours. (E) Effect of Y-27632 and Y-33075 on collagen gel contraction of culture-activated primary murine FVB/NJ cells (n = 3). (F) Representative images of gel contraction in FVB/NJ cells 24 hours after treatment with the ROCK inhibitors. (G) Protein expression levels of phospho-MLC and MLC in TWNT-4 after 24 hour incubation with the ROCK inhibitors. (H) Ratio of phospho-MLC to MLC in TWNT-4 cell lysates after treatment with ROCK inhibitors for 24 hours (n = 2). Results are expressed as the mean ± standard error of the mean (SEM); *p<0.05, **p<0.01 and ***p<0.001.
Y 39983 Hcl, supplied by Selleck Chemicals, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/y-39983/Y-39983+HCl/pm37174707-66-15-21
Average 92 stars, based on 1 article reviews
y 39983 hcl - by Bioz Stars, 2026-10
92/100 stars
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y39983 chemscene #cs-0096  (ChemScene llc)
90
ChemScene llc y39983 chemscene #cs-0096
(A) Schematic depiction of the RhoA-ROCK pathway including mechanism of action of the ROCK inhibitors Y-27632 and <t>Y-33075.</t> (B) Chemical structure of ROCK inhibitors Y-27632 and Y33075. (C) Effect of Y-27632 and Y-33075 on collagen gel contraction of activated human TWNT-4 cells (n = 3). (D) Representative images of the contraction assay in TWNT-4 cells after incubation with the ROCK inhibitors for 24 hours. (E) Effect of Y-27632 and Y-33075 on collagen gel contraction of culture-activated primary murine FVB/NJ cells (n = 3). (F) Representative images of gel contraction in FVB/NJ cells 24 hours after treatment with the ROCK inhibitors. (G) Protein expression levels of phospho-MLC and MLC in TWNT-4 after 24 hour incubation with the ROCK inhibitors. (H) Ratio of phospho-MLC to MLC in TWNT-4 cell lysates after treatment with ROCK inhibitors for 24 hours (n = 2). Results are expressed as the mean ± standard error of the mean (SEM); *p<0.05, **p<0.01 and ***p<0.001.
Y39983 Chemscene #Cs 0096, supplied by ChemScene llc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/y-39983/y39983+chemscene++cs+0096/us12091683-275-66-67
Average 90 stars, based on 1 article reviews
y39983 chemscene #cs-0096 - by Bioz Stars, 2026-10
90/100 stars
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rock1 inhibitor y39983 senju novartis  (Novartis)
86
Novartis rock1 inhibitor y39983 senju novartis
(A) Schematic depiction of the RhoA-ROCK pathway including mechanism of action of the ROCK inhibitors Y-27632 and <t>Y-33075.</t> (B) Chemical structure of ROCK inhibitors Y-27632 and Y33075. (C) Effect of Y-27632 and Y-33075 on collagen gel contraction of activated human TWNT-4 cells (n = 3). (D) Representative images of the contraction assay in TWNT-4 cells after incubation with the ROCK inhibitors for 24 hours. (E) Effect of Y-27632 and Y-33075 on collagen gel contraction of culture-activated primary murine FVB/NJ cells (n = 3). (F) Representative images of gel contraction in FVB/NJ cells 24 hours after treatment with the ROCK inhibitors. (G) Protein expression levels of phospho-MLC and MLC in TWNT-4 after 24 hour incubation with the ROCK inhibitors. (H) Ratio of phospho-MLC to MLC in TWNT-4 cell lysates after treatment with ROCK inhibitors for 24 hours (n = 2). Results are expressed as the mean ± standard error of the mean (SEM); *p<0.05, **p<0.01 and ***p<0.001.
Rock1 Inhibitor Y39983 Senju Novartis, supplied by Novartis, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/y-39983/inhibitor+novartis+rock1+senju+y39983/pm25589936__ml500367g_si_001-375-23-26
Average 86 stars, based on 1 article reviews
rock1 inhibitor y39983 senju novartis - by Bioz Stars, 2026-10
86/100 stars
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y-39983 hcl  (Aladdin Scientific Corporation)
N/A
InformationY-39983 HCl Y-39983 HCl (Y-33075) is a selective rho-associated protein kinase(ROCK) inhibitor with an IC50 of 3.6 nM.In vitroY-39983 opposes ROCK-dependent phosphorylation of MYPT1 predominantly at Thr853 with a corresponding decrease in MLC phosphorylation. It
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Image Search Results


(A) Schematic depiction of the RhoA-ROCK pathway including mechanism of action of the ROCK inhibitors Y-27632 and Y-33075. (B) Chemical structure of ROCK inhibitors Y-27632 and Y33075. (C) Effect of Y-27632 and Y-33075 on collagen gel contraction of activated human TWNT-4 cells (n = 3). (D) Representative images of the contraction assay in TWNT-4 cells after incubation with the ROCK inhibitors for 24 hours. (E) Effect of Y-27632 and Y-33075 on collagen gel contraction of culture-activated primary murine FVB/NJ cells (n = 3). (F) Representative images of gel contraction in FVB/NJ cells 24 hours after treatment with the ROCK inhibitors. (G) Protein expression levels of phospho-MLC and MLC in TWNT-4 after 24 hour incubation with the ROCK inhibitors. (H) Ratio of phospho-MLC to MLC in TWNT-4 cell lysates after treatment with ROCK inhibitors for 24 hours (n = 2). Results are expressed as the mean ± standard error of the mean (SEM); *p<0.05, **p<0.01 and ***p<0.001.

Journal: PLOS ONE

Article Title: The non-selective Rho-kinase inhibitors Y-27632 and Y-33075 decrease contraction but increase migration in murine and human hepatic stellate cells

doi: 10.1371/journal.pone.0270288

Figure Lengend Snippet: (A) Schematic depiction of the RhoA-ROCK pathway including mechanism of action of the ROCK inhibitors Y-27632 and Y-33075. (B) Chemical structure of ROCK inhibitors Y-27632 and Y33075. (C) Effect of Y-27632 and Y-33075 on collagen gel contraction of activated human TWNT-4 cells (n = 3). (D) Representative images of the contraction assay in TWNT-4 cells after incubation with the ROCK inhibitors for 24 hours. (E) Effect of Y-27632 and Y-33075 on collagen gel contraction of culture-activated primary murine FVB/NJ cells (n = 3). (F) Representative images of gel contraction in FVB/NJ cells 24 hours after treatment with the ROCK inhibitors. (G) Protein expression levels of phospho-MLC and MLC in TWNT-4 after 24 hour incubation with the ROCK inhibitors. (H) Ratio of phospho-MLC to MLC in TWNT-4 cell lysates after treatment with ROCK inhibitors for 24 hours (n = 2). Results are expressed as the mean ± standard error of the mean (SEM); *p<0.05, **p<0.01 and ***p<0.001.

Article Snippet: For these experiments Y-27632 dihydrochloride (Tocris, Wiesbaden, Germany) or Y-33075 (MedChemExpress, BIOZOL, Eching, Germany) was dissolved in PBS to create a stock solution at a concentration of 100mM.

Techniques: Contraction Assay, Incubation, Expressing

(A) Western blots of Col1a1, p-Moesin, Moesin and GAPDH using TWNT-4 cell lysates after 24 hour incubation with the ROCK inhibitors. (B) Quantification of protein expression of Col1a1 after treatment of TWNT-4 cells with ROCK inhibitors for 24 hours (n = 3). (C) Quantification of protein expression of Moesin after treatment of TWNT-4 cells with ROCK inhibitors for 24 hours (n = 3). (D) Quantification of protein expression of pMoesin after treatment of TWNT-4 cells with ROCK inhibitors for 24 hours (n = 3). (E) Western Blots of Col1a1, p-Moesin, Moesin and GAPDH using cell lysates of primary FVB/NJ cells after incubation with ROCK inhibitors for 24 hours. (F). Quantification of protein expression of Col1a1 after treatment of FVB/NJ cells with ROCK inhibitors for 24 hours (n = 3). (G) Quantification of protein expression of Moesin after treatment of FVB/NJ cells with ROCK inhibitors for 24 hours (n = 3). (H) Quantification of protein expression of p-Moesin after treatment of FVB/NJ cells with ROCK inhibitors for 24 hours (n = 3). (I) TWNT-4 mRNA expression levels of the pro-fibrotic marker Col1a1 and Tgfβ (corrected to 18s as a housekeeping gene) after 24h incubation with ROCK inhibitors Y-27632 and Y-33075 (n = 3). (J) FVB/NJ mRNA expression levels of the pro-fibrotic marker Col1a1 and Tgfβ (corrected to 18s as a housekeeping gene) after 24h incubation with ROCK inhibitors Y-27632 and Y-33075 (n = 3). Results are expressed as the mean ± standard error of the mean (SEM); *p<0.05, **p<0.01 and ***p<0.001.

Journal: PLOS ONE

Article Title: The non-selective Rho-kinase inhibitors Y-27632 and Y-33075 decrease contraction but increase migration in murine and human hepatic stellate cells

doi: 10.1371/journal.pone.0270288

Figure Lengend Snippet: (A) Western blots of Col1a1, p-Moesin, Moesin and GAPDH using TWNT-4 cell lysates after 24 hour incubation with the ROCK inhibitors. (B) Quantification of protein expression of Col1a1 after treatment of TWNT-4 cells with ROCK inhibitors for 24 hours (n = 3). (C) Quantification of protein expression of Moesin after treatment of TWNT-4 cells with ROCK inhibitors for 24 hours (n = 3). (D) Quantification of protein expression of pMoesin after treatment of TWNT-4 cells with ROCK inhibitors for 24 hours (n = 3). (E) Western Blots of Col1a1, p-Moesin, Moesin and GAPDH using cell lysates of primary FVB/NJ cells after incubation with ROCK inhibitors for 24 hours. (F). Quantification of protein expression of Col1a1 after treatment of FVB/NJ cells with ROCK inhibitors for 24 hours (n = 3). (G) Quantification of protein expression of Moesin after treatment of FVB/NJ cells with ROCK inhibitors for 24 hours (n = 3). (H) Quantification of protein expression of p-Moesin after treatment of FVB/NJ cells with ROCK inhibitors for 24 hours (n = 3). (I) TWNT-4 mRNA expression levels of the pro-fibrotic marker Col1a1 and Tgfβ (corrected to 18s as a housekeeping gene) after 24h incubation with ROCK inhibitors Y-27632 and Y-33075 (n = 3). (J) FVB/NJ mRNA expression levels of the pro-fibrotic marker Col1a1 and Tgfβ (corrected to 18s as a housekeeping gene) after 24h incubation with ROCK inhibitors Y-27632 and Y-33075 (n = 3). Results are expressed as the mean ± standard error of the mean (SEM); *p<0.05, **p<0.01 and ***p<0.001.

Article Snippet: For these experiments Y-27632 dihydrochloride (Tocris, Wiesbaden, Germany) or Y-33075 (MedChemExpress, BIOZOL, Eching, Germany) was dissolved in PBS to create a stock solution at a concentration of 100mM.

Techniques: Western Blot, Incubation, Expressing, Marker

(A) Effect of ROCK inhibitors Y-27632 and Y-33075 on wound closure in activated human TWNT-4 cells measured at 4, 8 and 24 hours (n = 3). (B) Representative images of the wound healing assay in TWNT-4 cells at 4, 8 and 24 hours after treatment with the ROCK inhibitors. The scale bars apply for all images and the length represents 200μm. (C) Effect of ROCK inhibitors Y-27632 and Y-33075 on wound closure in primary culture-activated FVB/NJ cells measured at 4, 8 and 24 hours (n = 3). (D) Representative images of the wound healing assay taken 4, 8 and 24 hours after treatment of FVB/NJ cells with ROCK inhibitors (scale bars, 200μm). Results are expressed as the mean ± standard error of the mean (SEM); *p<0.05, **p<0.01 and ***p<0.001.

Journal: PLOS ONE

Article Title: The non-selective Rho-kinase inhibitors Y-27632 and Y-33075 decrease contraction but increase migration in murine and human hepatic stellate cells

doi: 10.1371/journal.pone.0270288

Figure Lengend Snippet: (A) Effect of ROCK inhibitors Y-27632 and Y-33075 on wound closure in activated human TWNT-4 cells measured at 4, 8 and 24 hours (n = 3). (B) Representative images of the wound healing assay in TWNT-4 cells at 4, 8 and 24 hours after treatment with the ROCK inhibitors. The scale bars apply for all images and the length represents 200μm. (C) Effect of ROCK inhibitors Y-27632 and Y-33075 on wound closure in primary culture-activated FVB/NJ cells measured at 4, 8 and 24 hours (n = 3). (D) Representative images of the wound healing assay taken 4, 8 and 24 hours after treatment of FVB/NJ cells with ROCK inhibitors (scale bars, 200μm). Results are expressed as the mean ± standard error of the mean (SEM); *p<0.05, **p<0.01 and ***p<0.001.

Article Snippet: For these experiments Y-27632 dihydrochloride (Tocris, Wiesbaden, Germany) or Y-33075 (MedChemExpress, BIOZOL, Eching, Germany) was dissolved in PBS to create a stock solution at a concentration of 100mM.

Techniques: Wound Healing Assay

(A) Colorimetric BrdU evaluation of proliferation levels in TWNT-4 cells after 24h treatment with ROCK inhibitors (n = 3). (B) mRNA expression of Pcna as a marker for proliferation in TWNT-4 cells (corrected against 18s as a housekeeping gene) after 24 hour treatment with ROCK inhibitors. (C) Effect of 24 hour ROCK inhibitor treatments on proliferation in murine FVB/NJ cells assessed by colorimetric BrdU-Assay (n = 3). (D) mRNA expression of Pcna as a marker for proliferation in FVB/NJ cell lysates (corrected against 18s as a housekeeping gene) after 24 hour treatment with ROCK inhibitors (n = 3). (E) Protein expression of α-SMA as a marker for the activation of HSCs using cell lysates of human TWNT-4 cells after 24 hour treatment with Y-27632 and Y-33075. (F) Quantification of α-SMA protein expression after treatment of TWNT-4 cells with ROCK inhibitors for 24 hours (n = 3). (G) α-SMA protein expression in murine FVB/NJ cell lysates as a marker for activation in HSCs after 24 hour treatment with ROCK inhibitors. (H) Quantification of α-SMA protein expression after treatment of TWNT-4 cells with ROCK inhibitors for 24 hours (n = 3). Results are expressed as the mean ± standard error of the mean (SEM); *p<0.05, **p<0.01 and ***p<0.001.

Journal: PLOS ONE

Article Title: The non-selective Rho-kinase inhibitors Y-27632 and Y-33075 decrease contraction but increase migration in murine and human hepatic stellate cells

doi: 10.1371/journal.pone.0270288

Figure Lengend Snippet: (A) Colorimetric BrdU evaluation of proliferation levels in TWNT-4 cells after 24h treatment with ROCK inhibitors (n = 3). (B) mRNA expression of Pcna as a marker for proliferation in TWNT-4 cells (corrected against 18s as a housekeeping gene) after 24 hour treatment with ROCK inhibitors. (C) Effect of 24 hour ROCK inhibitor treatments on proliferation in murine FVB/NJ cells assessed by colorimetric BrdU-Assay (n = 3). (D) mRNA expression of Pcna as a marker for proliferation in FVB/NJ cell lysates (corrected against 18s as a housekeeping gene) after 24 hour treatment with ROCK inhibitors (n = 3). (E) Protein expression of α-SMA as a marker for the activation of HSCs using cell lysates of human TWNT-4 cells after 24 hour treatment with Y-27632 and Y-33075. (F) Quantification of α-SMA protein expression after treatment of TWNT-4 cells with ROCK inhibitors for 24 hours (n = 3). (G) α-SMA protein expression in murine FVB/NJ cell lysates as a marker for activation in HSCs after 24 hour treatment with ROCK inhibitors. (H) Quantification of α-SMA protein expression after treatment of TWNT-4 cells with ROCK inhibitors for 24 hours (n = 3). Results are expressed as the mean ± standard error of the mean (SEM); *p<0.05, **p<0.01 and ***p<0.001.

Article Snippet: For these experiments Y-27632 dihydrochloride (Tocris, Wiesbaden, Germany) or Y-33075 (MedChemExpress, BIOZOL, Eching, Germany) was dissolved in PBS to create a stock solution at a concentration of 100mM.

Techniques: Expressing, Marker, BrdU Staining, Activation Assay