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Image Search Results
Journal: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology
Article Title: Proline Absorption and SGK1 Expression are Inhibited in Intestinal Tis7 Transgenic Mice.
doi: 10.1159/000443094
Figure Lengend Snippet: Fig. 6. Reduced intestinal apical membrane expression of the proline-specific transporter SLC6A20 in tis7tg mice. (A) Immunoblot analysis of total cellular expression of the intestinal brush border protein sucra se-isomaltase (SI), and transporters for Glc (SGLT1), Pro (SLC6A20 and SLC36A1) and Leu (SLC6A19) in the supernatant of jejunum mucosa. (B) Expression in the apical membrane-bound fraction isolated through cell-surface biotinylation and analyzed by immunoblot. β-actin and SGLT1 served as the respective loading controls. Expression was quantified as the normalized densities of protein bands, as in (C) and (D), respecti vely. The inserted chart in (C) is a scale-adjusted quantification of SI, SGLT1, SLC6A19 and SLC6A20 protein expression. * p ≤ 0.05 WT vs. Tis7tg jejunum ; error bars represent the SEM.
Article Snippet: Anti-pThr256-SGK (sc16744R), anti-β-actin (sc47778), anti-SGLT1 (sc98974), anti-sucrase-isomaltase (SI) (sc27603), anti-SLC6A20 (sc134762), antiSLC6A19 (sc160812),
Techniques: Membrane, Expressing, Western Blot, Isolation