Journal: Biomolecules

Article Title: ABCD1 Transporter Deficiency Results in Altered Cholesterol Homeostasis

doi: 10.3390/biom13091333

Figure Lengend Snippet: X-ALD fibroblasts release higher amounts of cortisol upon stimulation with progesterone. Control (n = 6) or X-ALD (n = 10) fibroblasts were treated with vehicle (EtOH) or 1 μM progesterone for 24 h. Cortisol levels were measured in the supernatants using Luminex xMAP technology and normalised to the protein content of the harvested cells. The results are presented as box plots showing all values and the medians. Data were analysed by conducting one-way ANOVA with Sidak’s multiple comparisons test.

Article Snippet: Cortisol was measured using Luminex XMAP ® bead array technology (MSHMAG-21K, Merck) according to the manufacturer’s instructions.

Techniques: Luminex

Journal: International Journal of Molecular Sciences

Article Title: Inhaled CD24-Enriched Exosomes (EXO-CD24) as a Novel Immune Modulator in Respiratory Disease

doi: 10.3390/ijms25010077

Figure Lengend Snippet: Inflammatory indices in a mice ARDS model. The levels of local (BALF, ( A )) and systemic (serum, ( B )) cytokines and chemokines were measured using the Luminex’s xMAP cytokine multiplex array. Data are represented as average ± SEM; n = 10 mice/group. ( C ) Histology. Left: moderate to severe lesion, including neutrophil infiltration, fibrin deposition, and alveolar wall thickening (H&E ×10). Middle: Dexa + EXO-CD24-treated animal, mild to moderate lesions, including decreased neutrophil infiltration, decreased fibrin deposition, and less alveolar wall thickening compared to the other treated groups (H&E. ×10). Right: naïve animal, very mild lesions (H&E. ×10).

Article Snippet: MILLIPLEX ® Mouse Cytokine/Chemokine Magnetic Bead Panels (Millipore, Merck, Rosh-Ha’ayin, Israel) based on the Luminex ® xMAP ® technology (Merck, Rosh-Ha’ayin, Israel) were used.

Techniques: Multiplex Assay

Journal: International Journal of Molecular Sciences

Article Title: Inhaled CD24-Enriched Exosomes (EXO-CD24) as a Novel Immune Modulator in Respiratory Disease

doi: 10.3390/ijms25010077

Figure Lengend Snippet: ( A ). Cell count in mice’ broncho alveolar lavage fluid. Cells from the BALF tissues were analyzed for the differential cell counts of BALF cells by flow cytometry for eosinophils, B cells, T cells, and neutrophils. ( B ). Survival of mice. Pulmonary sepsis was induced, followed by an inhalation treatment with vehicle or 10 10 EXO-mCD24/mouse. ( C ). BALF total protein concentration. The total protein concentration of cell-free BALF fluid was determined. The graph depicts the mean protein concentration values, and standard deviations are indicated by error bars. ( D ). The levels of secreted cytokines and chemokines were measured using the Luminex’s xMAP cytokine “Multi-plex” array. Data are represented as average ± SEM; n = 10 mice/group.

Article Snippet: MILLIPLEX ® Mouse Cytokine/Chemokine Magnetic Bead Panels (Millipore, Merck, Rosh-Ha’ayin, Israel) based on the Luminex ® xMAP ® technology (Merck, Rosh-Ha’ayin, Israel) were used.

Techniques: Cell Counting, Flow Cytometry, Protein Concentration

Journal: International Journal of Molecular Sciences

Article Title: Inhaled CD24-Enriched Exosomes (EXO-CD24) as a Novel Immune Modulator in Respiratory Disease

doi: 10.3390/ijms25010077

Figure Lengend Snippet: Histological images comparing inflammatory infiltrates between the untreated and EXO-mCD24-treated mice with induced Asthma. ( A ) Left: untreated mouse. Moderate inflammatory infiltration. Rectangle—the area enlarged in the following figure (H&E ×10). Right: treated mouse. Mild inflammatory infiltration. Rectangle—the area enlarged in the following figure (H&E ×10). ( B ) The levels of secreted cytokines and chemokines were measured using the Luminex’s xMAP cytokine multiplex array. ( C ) The levels of IgE were measured. Data are represented as average ± SEM; n = 10 mice/group.

Article Snippet: MILLIPLEX ® Mouse Cytokine/Chemokine Magnetic Bead Panels (Millipore, Merck, Rosh-Ha’ayin, Israel) based on the Luminex ® xMAP ® technology (Merck, Rosh-Ha’ayin, Israel) were used.

Techniques: Multiplex Assay

Journal: International Journal of Molecular Sciences

Article Title: Inhaled CD24-Enriched Exosomes (EXO-CD24) as a Novel Immune Modulator in Respiratory Disease

doi: 10.3390/ijms25010077

Figure Lengend Snippet: ( A ) Cell composition in broncho alveolar lavage fluid. Bronchial Alveolar Lavage Fluid (BALF) differential cell counting was performed by flow cytometry for T and B lymphocytes, eosinophils, neutrophils, dendritic cells, and monocytes/macrophages. ( B ) The levels of secreted cytokines and chemokines were measured using the Luminex’s xMAP cytokine “Multi-plex” array. Data are represented as average ± SEM; n = 10 mice/group. ( C ) Representative pictures of the affected areas in the lungs. ×20; H&E, MT (Masson’s trichrome). Upper panel: histopathological findings (H&E); bottom panel: Masson’s trichrome staining for fibrosis.

Article Snippet: MILLIPLEX ® Mouse Cytokine/Chemokine Magnetic Bead Panels (Millipore, Merck, Rosh-Ha’ayin, Israel) based on the Luminex ® xMAP ® technology (Merck, Rosh-Ha’ayin, Israel) were used.

Techniques: Cell Counting, Flow Cytometry, Staining