xfect Search Results


transfections  (TaKaRa)
97
TaKaRa transfections
Transfections, supplied by TaKaRa, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/transfections/product/TaKaRa
Average 97 stars, based on 1 article reviews
transfections - by Bioz Stars, 2026-03
97/100 stars
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sirna transfection  (TaKaRa)
95
TaKaRa sirna transfection
Sirna Transfection, supplied by TaKaRa, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sirna transfection/product/TaKaRa
Average 95 stars, based on 1 article reviews
sirna transfection - by Bioz Stars, 2026-03
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xfect mesc transfection reagent  (TaKaRa)
94
TaKaRa xfect mesc transfection reagent
dCas9VP160 activates endogenous genes. (A) Protein architecture of dCas9VP160 compared to VP48. (B) Schematic of the human IL1RN promoter region. Locations of transcription start site (TSS) and start codon (ATG) are indicated. Short lines with number indicate targeting sites of the sgRNAs. (C) Activation of human IL1RN expression in HEK293T cells. Cells were analyzed by qRT-PCR 2 days after <t>transfection</t> with dCas9VP160 and the indicated sgRNAs. (D) Schematic of the human SOX2 promoter region. Locations of TSS and start codon (ATG) are indicated. Short lines with number indicate targeting sites of sgRNAs. (E) Activation of SOX2 . Cells were analyzed by qRT-PCR 2 days after transfection with dCas9VP160 and the indicated sgRNAs. (F) Schematic of the human OCT4 promoter region. Locations of transcription start site (TSS) and start codon (ATG) are indicated. Short lines with number indicate targeting sites of sgRNAs. (G) Activation of OCT4 . Cells transfected with dCas9VP160 and the indicated sgRNAs were analyzed by qRT-PCR 2 days later. sgTetO-mut, negative control sgRNA. Error bars show SD among triplicates.
Xfect Mesc Transfection Reagent, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/xfect mesc transfection reagent/product/TaKaRa
Average 94 stars, based on 1 article reviews
xfect mesc transfection reagent - by Bioz Stars, 2026-03
94/100 stars
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xfect protein transfection reagent  (TaKaRa)
93
TaKaRa xfect protein transfection reagent
dCas9VP160 activates endogenous genes. (A) Protein architecture of dCas9VP160 compared to VP48. (B) Schematic of the human IL1RN promoter region. Locations of transcription start site (TSS) and start codon (ATG) are indicated. Short lines with number indicate targeting sites of the sgRNAs. (C) Activation of human IL1RN expression in HEK293T cells. Cells were analyzed by qRT-PCR 2 days after <t>transfection</t> with dCas9VP160 and the indicated sgRNAs. (D) Schematic of the human SOX2 promoter region. Locations of TSS and start codon (ATG) are indicated. Short lines with number indicate targeting sites of sgRNAs. (E) Activation of SOX2 . Cells were analyzed by qRT-PCR 2 days after transfection with dCas9VP160 and the indicated sgRNAs. (F) Schematic of the human OCT4 promoter region. Locations of transcription start site (TSS) and start codon (ATG) are indicated. Short lines with number indicate targeting sites of sgRNAs. (G) Activation of OCT4 . Cells transfected with dCas9VP160 and the indicated sgRNAs were analyzed by qRT-PCR 2 days later. sgTetO-mut, negative control sgRNA. Error bars show SD among triplicates.
Xfect Protein Transfection Reagent, supplied by TaKaRa, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/xfect protein transfection reagent/product/TaKaRa
Average 93 stars, based on 1 article reviews
xfect protein transfection reagent - by Bioz Stars, 2026-03
93/100 stars
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xfect tm transfection reagent  (Takeda)
90
Takeda xfect tm transfection reagent
dCas9VP160 activates endogenous genes. (A) Protein architecture of dCas9VP160 compared to VP48. (B) Schematic of the human IL1RN promoter region. Locations of transcription start site (TSS) and start codon (ATG) are indicated. Short lines with number indicate targeting sites of the sgRNAs. (C) Activation of human IL1RN expression in HEK293T cells. Cells were analyzed by qRT-PCR 2 days after <t>transfection</t> with dCas9VP160 and the indicated sgRNAs. (D) Schematic of the human SOX2 promoter region. Locations of TSS and start codon (ATG) are indicated. Short lines with number indicate targeting sites of sgRNAs. (E) Activation of SOX2 . Cells were analyzed by qRT-PCR 2 days after transfection with dCas9VP160 and the indicated sgRNAs. (F) Schematic of the human OCT4 promoter region. Locations of transcription start site (TSS) and start codon (ATG) are indicated. Short lines with number indicate targeting sites of sgRNAs. (G) Activation of OCT4 . Cells transfected with dCas9VP160 and the indicated sgRNAs were analyzed by qRT-PCR 2 days later. sgTetO-mut, negative control sgRNA. Error bars show SD among triplicates.
Xfect Tm Transfection Reagent, supplied by Takeda, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/xfect tm transfection reagent/product/Takeda
Average 90 stars, based on 1 article reviews
xfect tm transfection reagent - by Bioz Stars, 2026-03
90/100 stars
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xfect tm polymer reagent  (Becton Dickinson)
90
Becton Dickinson xfect tm polymer reagent
dCas9VP160 activates endogenous genes. (A) Protein architecture of dCas9VP160 compared to VP48. (B) Schematic of the human IL1RN promoter region. Locations of transcription start site (TSS) and start codon (ATG) are indicated. Short lines with number indicate targeting sites of the sgRNAs. (C) Activation of human IL1RN expression in HEK293T cells. Cells were analyzed by qRT-PCR 2 days after <t>transfection</t> with dCas9VP160 and the indicated sgRNAs. (D) Schematic of the human SOX2 promoter region. Locations of TSS and start codon (ATG) are indicated. Short lines with number indicate targeting sites of sgRNAs. (E) Activation of SOX2 . Cells were analyzed by qRT-PCR 2 days after transfection with dCas9VP160 and the indicated sgRNAs. (F) Schematic of the human OCT4 promoter region. Locations of transcription start site (TSS) and start codon (ATG) are indicated. Short lines with number indicate targeting sites of sgRNAs. (G) Activation of OCT4 . Cells transfected with dCas9VP160 and the indicated sgRNAs were analyzed by qRT-PCR 2 days later. sgTetO-mut, negative control sgRNA. Error bars show SD among triplicates.
Xfect Tm Polymer Reagent, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/xfect tm polymer reagent/product/Becton Dickinson
Average 90 stars, based on 1 article reviews
xfect tm polymer reagent - by Bioz Stars, 2026-03
90/100 stars
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xfect  (ScreenFect GmbH)
90
ScreenFect GmbH xfect
dCas9VP160 activates endogenous genes. (A) Protein architecture of dCas9VP160 compared to VP48. (B) Schematic of the human IL1RN promoter region. Locations of transcription start site (TSS) and start codon (ATG) are indicated. Short lines with number indicate targeting sites of the sgRNAs. (C) Activation of human IL1RN expression in HEK293T cells. Cells were analyzed by qRT-PCR 2 days after <t>transfection</t> with dCas9VP160 and the indicated sgRNAs. (D) Schematic of the human SOX2 promoter region. Locations of TSS and start codon (ATG) are indicated. Short lines with number indicate targeting sites of sgRNAs. (E) Activation of SOX2 . Cells were analyzed by qRT-PCR 2 days after transfection with dCas9VP160 and the indicated sgRNAs. (F) Schematic of the human OCT4 promoter region. Locations of transcription start site (TSS) and start codon (ATG) are indicated. Short lines with number indicate targeting sites of sgRNAs. (G) Activation of OCT4 . Cells transfected with dCas9VP160 and the indicated sgRNAs were analyzed by qRT-PCR 2 days later. sgTetO-mut, negative control sgRNA. Error bars show SD among triplicates.
Xfect, supplied by ScreenFect GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/xfect/product/ScreenFect GmbH
Average 90 stars, based on 1 article reviews
xfect - by Bioz Stars, 2026-03
90/100 stars
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xfect transfection reagent  (Contech Enterprises Inc)
90
Contech Enterprises Inc xfect transfection reagent
dCas9VP160 activates endogenous genes. (A) Protein architecture of dCas9VP160 compared to VP48. (B) Schematic of the human IL1RN promoter region. Locations of transcription start site (TSS) and start codon (ATG) are indicated. Short lines with number indicate targeting sites of the sgRNAs. (C) Activation of human IL1RN expression in HEK293T cells. Cells were analyzed by qRT-PCR 2 days after <t>transfection</t> with dCas9VP160 and the indicated sgRNAs. (D) Schematic of the human SOX2 promoter region. Locations of TSS and start codon (ATG) are indicated. Short lines with number indicate targeting sites of sgRNAs. (E) Activation of SOX2 . Cells were analyzed by qRT-PCR 2 days after transfection with dCas9VP160 and the indicated sgRNAs. (F) Schematic of the human OCT4 promoter region. Locations of transcription start site (TSS) and start codon (ATG) are indicated. Short lines with number indicate targeting sites of sgRNAs. (G) Activation of OCT4 . Cells transfected with dCas9VP160 and the indicated sgRNAs were analyzed by qRT-PCR 2 days later. sgTetO-mut, negative control sgRNA. Error bars show SD among triplicates.
Xfect Transfection Reagent, supplied by Contech Enterprises Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/xfect transfection reagent/product/Contech Enterprises Inc
Average 90 stars, based on 1 article reviews
xfect transfection reagent - by Bioz Stars, 2026-03
90/100 stars
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xfect tm transfection reagent  (Becton Dickinson)
90
Becton Dickinson xfect tm transfection reagent
dCas9VP160 activates endogenous genes. (A) Protein architecture of dCas9VP160 compared to VP48. (B) Schematic of the human IL1RN promoter region. Locations of transcription start site (TSS) and start codon (ATG) are indicated. Short lines with number indicate targeting sites of the sgRNAs. (C) Activation of human IL1RN expression in HEK293T cells. Cells were analyzed by qRT-PCR 2 days after <t>transfection</t> with dCas9VP160 and the indicated sgRNAs. (D) Schematic of the human SOX2 promoter region. Locations of TSS and start codon (ATG) are indicated. Short lines with number indicate targeting sites of sgRNAs. (E) Activation of SOX2 . Cells were analyzed by qRT-PCR 2 days after transfection with dCas9VP160 and the indicated sgRNAs. (F) Schematic of the human OCT4 promoter region. Locations of transcription start site (TSS) and start codon (ATG) are indicated. Short lines with number indicate targeting sites of sgRNAs. (G) Activation of OCT4 . Cells transfected with dCas9VP160 and the indicated sgRNAs were analyzed by qRT-PCR 2 days later. sgTetO-mut, negative control sgRNA. Error bars show SD among triplicates.
Xfect Tm Transfection Reagent, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/xfect tm transfection reagent/product/Becton Dickinson
Average 90 stars, based on 1 article reviews
xfect tm transfection reagent - by Bioz Stars, 2026-03
90/100 stars
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xfect transfection reagent  (Genentech inc)
90
Genentech inc xfect transfection reagent
dCas9VP160 activates endogenous genes. (A) Protein architecture of dCas9VP160 compared to VP48. (B) Schematic of the human IL1RN promoter region. Locations of transcription start site (TSS) and start codon (ATG) are indicated. Short lines with number indicate targeting sites of the sgRNAs. (C) Activation of human IL1RN expression in HEK293T cells. Cells were analyzed by qRT-PCR 2 days after <t>transfection</t> with dCas9VP160 and the indicated sgRNAs. (D) Schematic of the human SOX2 promoter region. Locations of TSS and start codon (ATG) are indicated. Short lines with number indicate targeting sites of sgRNAs. (E) Activation of SOX2 . Cells were analyzed by qRT-PCR 2 days after transfection with dCas9VP160 and the indicated sgRNAs. (F) Schematic of the human OCT4 promoter region. Locations of transcription start site (TSS) and start codon (ATG) are indicated. Short lines with number indicate targeting sites of sgRNAs. (G) Activation of OCT4 . Cells transfected with dCas9VP160 and the indicated sgRNAs were analyzed by qRT-PCR 2 days later. sgTetO-mut, negative control sgRNA. Error bars show SD among triplicates.
Xfect Transfection Reagent, supplied by Genentech inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/xfect transfection reagent/product/Genentech inc
Average 90 stars, based on 1 article reviews
xfect transfection reagent - by Bioz Stars, 2026-03
90/100 stars
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xfect buffer  (Corning Life Sciences)
90
Corning Life Sciences xfect buffer
dCas9VP160 activates endogenous genes. (A) Protein architecture of dCas9VP160 compared to VP48. (B) Schematic of the human IL1RN promoter region. Locations of transcription start site (TSS) and start codon (ATG) are indicated. Short lines with number indicate targeting sites of the sgRNAs. (C) Activation of human IL1RN expression in HEK293T cells. Cells were analyzed by qRT-PCR 2 days after <t>transfection</t> with dCas9VP160 and the indicated sgRNAs. (D) Schematic of the human SOX2 promoter region. Locations of TSS and start codon (ATG) are indicated. Short lines with number indicate targeting sites of sgRNAs. (E) Activation of SOX2 . Cells were analyzed by qRT-PCR 2 days after transfection with dCas9VP160 and the indicated sgRNAs. (F) Schematic of the human OCT4 promoter region. Locations of transcription start site (TSS) and start codon (ATG) are indicated. Short lines with number indicate targeting sites of sgRNAs. (G) Activation of OCT4 . Cells transfected with dCas9VP160 and the indicated sgRNAs were analyzed by qRT-PCR 2 days later. sgTetO-mut, negative control sgRNA. Error bars show SD among triplicates.
Xfect Buffer, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/xfect buffer/product/Corning Life Sciences
Average 90 stars, based on 1 article reviews
xfect buffer - by Bioz Stars, 2026-03
90/100 stars
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xfect  (Sinapse Inc)
90
Sinapse Inc xfect
dCas9VP160 activates endogenous genes. (A) Protein architecture of dCas9VP160 compared to VP48. (B) Schematic of the human IL1RN promoter region. Locations of transcription start site (TSS) and start codon (ATG) are indicated. Short lines with number indicate targeting sites of the sgRNAs. (C) Activation of human IL1RN expression in HEK293T cells. Cells were analyzed by qRT-PCR 2 days after <t>transfection</t> with dCas9VP160 and the indicated sgRNAs. (D) Schematic of the human SOX2 promoter region. Locations of TSS and start codon (ATG) are indicated. Short lines with number indicate targeting sites of sgRNAs. (E) Activation of SOX2 . Cells were analyzed by qRT-PCR 2 days after transfection with dCas9VP160 and the indicated sgRNAs. (F) Schematic of the human OCT4 promoter region. Locations of transcription start site (TSS) and start codon (ATG) are indicated. Short lines with number indicate targeting sites of sgRNAs. (G) Activation of OCT4 . Cells transfected with dCas9VP160 and the indicated sgRNAs were analyzed by qRT-PCR 2 days later. sgTetO-mut, negative control sgRNA. Error bars show SD among triplicates.
Xfect, supplied by Sinapse Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/xfect/product/Sinapse Inc
Average 90 stars, based on 1 article reviews
xfect - by Bioz Stars, 2026-03
90/100 stars
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Image Search Results


dCas9VP160 activates endogenous genes. (A) Protein architecture of dCas9VP160 compared to VP48. (B) Schematic of the human IL1RN promoter region. Locations of transcription start site (TSS) and start codon (ATG) are indicated. Short lines with number indicate targeting sites of the sgRNAs. (C) Activation of human IL1RN expression in HEK293T cells. Cells were analyzed by qRT-PCR 2 days after transfection with dCas9VP160 and the indicated sgRNAs. (D) Schematic of the human SOX2 promoter region. Locations of TSS and start codon (ATG) are indicated. Short lines with number indicate targeting sites of sgRNAs. (E) Activation of SOX2 . Cells were analyzed by qRT-PCR 2 days after transfection with dCas9VP160 and the indicated sgRNAs. (F) Schematic of the human OCT4 promoter region. Locations of transcription start site (TSS) and start codon (ATG) are indicated. Short lines with number indicate targeting sites of sgRNAs. (G) Activation of OCT4 . Cells transfected with dCas9VP160 and the indicated sgRNAs were analyzed by qRT-PCR 2 days later. sgTetO-mut, negative control sgRNA. Error bars show SD among triplicates.

Journal: Cell Research

Article Title: Multiplexed activation of endogenous genes by CRISPR-on, an RNA-guided transcriptional activator system

doi: 10.1038/cr.2013.122

Figure Lengend Snippet: dCas9VP160 activates endogenous genes. (A) Protein architecture of dCas9VP160 compared to VP48. (B) Schematic of the human IL1RN promoter region. Locations of transcription start site (TSS) and start codon (ATG) are indicated. Short lines with number indicate targeting sites of the sgRNAs. (C) Activation of human IL1RN expression in HEK293T cells. Cells were analyzed by qRT-PCR 2 days after transfection with dCas9VP160 and the indicated sgRNAs. (D) Schematic of the human SOX2 promoter region. Locations of TSS and start codon (ATG) are indicated. Short lines with number indicate targeting sites of sgRNAs. (E) Activation of SOX2 . Cells were analyzed by qRT-PCR 2 days after transfection with dCas9VP160 and the indicated sgRNAs. (F) Schematic of the human OCT4 promoter region. Locations of transcription start site (TSS) and start codon (ATG) are indicated. Short lines with number indicate targeting sites of sgRNAs. (G) Activation of OCT4 . Cells transfected with dCas9VP160 and the indicated sgRNAs were analyzed by qRT-PCR 2 days later. sgTetO-mut, negative control sgRNA. Error bars show SD among triplicates.

Article Snippet: mESCs from mice carrying a Dox-inducible Musashi-1 (MSI1) allele in the Col1A1 locus were transfected with dCas9VP48 using Xfect mESC transfection reagent (Clontech) or were cultured in mouse ES medium with 2 μg/ml Doxycycline.

Techniques: Activation Assay, Expressing, Quantitative RT-PCR, Transfection, Negative Control

Multiple exogenous and endogenous genes were simultaneously activated by CRISPR-on. (A) One exogenous and two endogenous genes were simultaneously activated by CRISPR-on. Cells were analyzed by qRT-PCR 2 days after transfection with dCas9VP160 and the indicated sgRNAs. (B) Three endogenous genes, SOX2 , IL1RN and OCT4 , can be simultaneously activated by dCas9VP160/sgRNAs. Cells were transfected with dCas9VP160 and the indicated sgRNAs and were analyzed by qRT-PCR 2 days after transfection. The Last three sets of bars represent triple activation experiments using sgSOX2, sgOCT4 and sgIL1RN with three different ratios of sgSOX2:sgIL1RN, keeping the amount of sgOCT4 constant, as indicated by numbers above line. sgTetO-mut, negative control sgRNA. Error bars show SD among triplicates.

Journal: Cell Research

Article Title: Multiplexed activation of endogenous genes by CRISPR-on, an RNA-guided transcriptional activator system

doi: 10.1038/cr.2013.122

Figure Lengend Snippet: Multiple exogenous and endogenous genes were simultaneously activated by CRISPR-on. (A) One exogenous and two endogenous genes were simultaneously activated by CRISPR-on. Cells were analyzed by qRT-PCR 2 days after transfection with dCas9VP160 and the indicated sgRNAs. (B) Three endogenous genes, SOX2 , IL1RN and OCT4 , can be simultaneously activated by dCas9VP160/sgRNAs. Cells were transfected with dCas9VP160 and the indicated sgRNAs and were analyzed by qRT-PCR 2 days after transfection. The Last three sets of bars represent triple activation experiments using sgSOX2, sgOCT4 and sgIL1RN with three different ratios of sgSOX2:sgIL1RN, keeping the amount of sgOCT4 constant, as indicated by numbers above line. sgTetO-mut, negative control sgRNA. Error bars show SD among triplicates.

Article Snippet: mESCs from mice carrying a Dox-inducible Musashi-1 (MSI1) allele in the Col1A1 locus were transfected with dCas9VP48 using Xfect mESC transfection reagent (Clontech) or were cultured in mouse ES medium with 2 μg/ml Doxycycline.

Techniques: CRISPR, Quantitative RT-PCR, Transfection, Activation Assay, Negative Control