Journal: Clinical Science

Article Title: Xbp1s-Ddit3 promotes MCT-induced pulmonary hypertension

doi: 10.1042/cs20210612

Figure Lengend Snippet: Figure 1. Knockdown of xbp1s mitigated MCT-induced PH Forty SD male rats were randomly assigned to four groups. MCT was administered through intraperitoneal injection, and AAV was

Article Snippet: Xbp1s and rabbit IgG were immunoprecipitated from lysates with the corresponding primary antibodies: Xbp1s (CST, 40435s) and rabbit IgG (Beyotime, A7016).

Techniques: Knockdown, Injection

Journal: Clinical Science

Article Title: Xbp1s-Ddit3 promotes MCT-induced pulmonary hypertension

doi: 10.1042/cs20210612

Figure Lengend Snippet: Figure 2. Knockdown of xbp1s alleviated MCT-induced vascular remodeling in the PH model (A) Representative image of HE staining, which was performed to quantify the medial wall thickness of the distal pulmonary ar-

Article Snippet: Xbp1s and rabbit IgG were immunoprecipitated from lysates with the corresponding primary antibodies: Xbp1s (CST, 40435s) and rabbit IgG (Beyotime, A7016).

Techniques: Knockdown, Staining

Journal: Clinical Science

Article Title: Xbp1s-Ddit3 promotes MCT-induced pulmonary hypertension

doi: 10.1042/cs20210612

Figure Lengend Snippet: Figure 3. Knockdown of xbp1s restored MCT-induced proliferation and antiapoptotic effects in the PH model (A) Representative image of immunohistochemical staining of PCNA and Ki67, which both reflect the proliferation of the detected sample. (B) Representative image of TUNEL staining. TUNEL-positive staining reflects the apoptotic state of the detected sample.

Article Snippet: Xbp1s and rabbit IgG were immunoprecipitated from lysates with the corresponding primary antibodies: Xbp1s (CST, 40435s) and rabbit IgG (Beyotime, A7016).

Techniques: Knockdown, Immunohistochemical staining, Staining, TUNEL Assay

Journal: Clinical Science

Article Title: Xbp1s-Ddit3 promotes MCT-induced pulmonary hypertension

doi: 10.1042/cs20210612

Figure Lengend Snippet: Figure 4. Proteins that interact with xbp1s (A) Image showing the protein interaction network focused on xbp1s. Data were predicted by the STRING database. (B) qRT-PCR results showing the relative expression of proteins predicted to interact with xbp1s. Expression was detected in PASMCs cultured

Article Snippet: Xbp1s and rabbit IgG were immunoprecipitated from lysates with the corresponding primary antibodies: Xbp1s (CST, 40435s) and rabbit IgG (Beyotime, A7016).

Techniques: Quantitative RT-PCR, Expressing, Cell Culture

Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

Article Title: miR-512-3p Overcomes Resistance to Cisplatin in Retinoblastoma by Promoting Apoptosis Induced by Endoplasmic Reticulum Stress

doi: 10.12659/MSM.923817

Figure Lengend Snippet: miR-512-3p affects the related proteins expression in ERS. ( A ) The expression of GRP78, ATF4, ATF6 and IRE1 in Y79/DDP cells after transfection was determined by Western blot analysis. * P<0.05 and ** P<0.05 vs. Y79 group. # P<0.05, ## P<0.01 and ### P<0.001 vs. Y79/DDP group. && P<0.01 and &&& P<0.001 vs. Y79/DDP+mimic NC group. $$$ P<0.001 vs. Y79/DDP+inhibitor NC group. ( B ) The expression of CHOP, XBP-1s, XBP-1u and Casp-4 in Y79/DDP cells after transfection was determined by Western blot analysis. *** P<0.001 vs. Y79 group. # P<0.05 and ### P<0.001 vs. Y79/DDP group. & P<0.05 and &&& P<0.001 vs. Y79/DDP+mimic NC group. $ P<0.05 vs. Y79/DDP+inhibitor NC group.

Article Snippet: The PVDF membrane was incubated with primary antibody at 4°C overnight, and with secondary antibody at 37°C for 1 h. GAPDH was used as an internal control for the detection of XBP-1 (ab37152, Abcam), LC3I/II (ab62721, Abcam), GRP78 (ab21685, Abcam), ATF4 (ab184909, Abcam), ATF6 (ab203119, Abcam), IRE1 (ab37073, Abcam), CHOP (#2895, Cell Signaling Technology), XBP-1s (#27901, Cell Signaling Technology), XBP-1u (25997-1-AP, ProteinTech), Casp-4 (ab25898, Abcam), casp-3 (ab13847, Abcam), c-casp-3 (ab2302, Abcam), Bax (ab32503, Abcam), Bcl-2 (ab32124, Abcam), Beclin-1 (ab207612, Abcam) and Atg5 (#2630, Cell Signaling Technology).

Techniques: Expressing, Transfection, Western Blot