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Image Search Results
Journal: European Journal of Histochemistry : EJH
Article Title: SRSF3 promotes the generation of XBP1s to stabilize autophagy and enhance hypoxia adaptation in glioma
doi: 10.4081/ejh.2026.4530
Figure Lengend Snippet: Hypoxic glioma cells exhibit increased cell death accompanied by elevated endoplasmic reticulum stress levels and blocked autophagic flux. A ) Western blot analysis of HIF-1α expression in U87 cells. B ) LDH release assay to detect cell death. C ) Western blot analysis of cleaved-caspase3 expression in U87 cells. D ) Western blot analysis of GRP78, CHOP, and ATF4 expression in U87 cells. E ) RT-PCR detection of XBP1u and XBP1s expression. F ) Western blot analysis of XBP1u and XBP1s expression in U87 cells. G ) Western blot analysis of LC3-II and p62 expression in U87 cells. H ) mRFP-GFP-LC3 staining to assess autophagic flux. n=3 independent experiments; * p <0.05, ** p <0.01, *** p <0.001.
Article Snippet: The primary antibodies used were against the following: SRSF3 (1:1000; ab198291; Abcam, Cambridge, UK), HIF-1α (1:1000; ab179483; Abcam), GRP78 (1:2000; 11587-1-AP; Proteintech, Rosemont, IL, USA), CHOP (1:1000; 15204-1-AP; Proteintech), ATF4 (1:1000; 10835-1-AP; Proteintech), LC3 (1:2000; ab192890; Abcam), p62 (1:1000; ab109012; Abcam), cleaved-caspase3 (1:1000; #9661; Cell Signaling Technology, Inc., Danvers, MA, USA), XBP1u (1:1000; 25997-1-AP; Proteintech),
Techniques: Western Blot, Expressing, Lactate Dehydrogenase Assay, Reverse Transcription Polymerase Chain Reaction, Staining
Journal: European Journal of Histochemistry : EJH
Article Title: SRSF3 promotes the generation of XBP1s to stabilize autophagy and enhance hypoxia adaptation in glioma
doi: 10.4081/ejh.2026.4530
Figure Lengend Snippet: SRSF3 promotes XBP1s formation. A ) RT‒PCR detection of XBP1u and XBP1s expression in each group of cells. B ) Western blot detection of XBP1u and XBP1s expression in each group of cells. n=3 independent experiments; * p <0.05, ** p <0.01, *** p <0.001.
Article Snippet: The primary antibodies used were against the following: SRSF3 (1:1000; ab198291; Abcam, Cambridge, UK), HIF-1α (1:1000; ab179483; Abcam), GRP78 (1:2000; 11587-1-AP; Proteintech, Rosemont, IL, USA), CHOP (1:1000; 15204-1-AP; Proteintech), ATF4 (1:1000; 10835-1-AP; Proteintech), LC3 (1:2000; ab192890; Abcam), p62 (1:1000; ab109012; Abcam), cleaved-caspase3 (1:1000; #9661; Cell Signaling Technology, Inc., Danvers, MA, USA), XBP1u (1:1000; 25997-1-AP; Proteintech),
Techniques: Expressing, Western Blot