Journal: Nature biomedical engineering

Article Title: Generation of neural organoids for spinal-cord regeneration via the direct reprogramming of human astrocytes

doi: 10.1038/s41551-022-00963-6

Figure Lengend Snippet: a , Schematic for the direct reprogramming of human astrocytes into neurons. b , Schematic diagram of primary screening protocol to convert human astrocytes into neurons using each of 15 small molecules. AM, Astrocyte Medium; NB, Neurobasal medium. c , Representative images of MAP2 + cells treated with each of 15 small molecules and DMSO as control at day 21. Scale bar, 100 μm. d , Quantification of the percentage of induced MAP2 + neurons treated with 15 small molecules (CHIR99021, Y27632, SB431542, XAV939 and SAG, n = 4 independent experiments; the rest, n = 3 independent experiments) and DMSO (n = 4 independent experiments). Data are presented as mean ± SEM.

Article Snippet: From day 3 to day 8, small molecules including CHIR99021 (1.5 μM, Tocris), VPA (5 mM, Cayman), RepSox (1 μM, Tocris), Forskolin (10 μM, Cayman), JQ1 (50 nM, Sigma), ISX-9 (10 μM, Tocris), LDN193189 (100 nM, Stemolecule), TTNPB (0.5 μM, Tocris), Y27632 (10 μM, Tocris), DAPT (2.5 μM, Sigma), SAG (100 nM, EMD Millipore), SB431542 (10 μM, MCE), PD0325901 (10 μM, Tocris), SU5402 (10 μM, Biovision), and XAV939 (10 μM, Tocris) were added to induce neurons cultured with Neural Medium for primary screening.

Techniques: Control

Journal: Cell Reports Methods

Article Title: Patterning ganglionic eminences in developing human brain organoids using a morphogen-gradient-inducing device

doi: 10.1016/j.crmeth.2023.100689

Figure Lengend Snippet:

Article Snippet: XAV939 [5 μM] , BioGems , 2848932-25mg.

Techniques: Recombinant, Gene Expression, Software, Imaging

Journal: Oncology reports

Article Title: TRIM52 regulates the proliferation and invasiveness of lung cancer cells via the Wnt/β‑catenin pathway.

doi: 10.3892/or.2019.7110

Figure Lengend Snippet: Figure 4. TRIM52 regulates proliferation, cell cycle and invasion through the Wnt/β‑catenin pathway. H1975 and A549 cells were treated with vector/oeTRIM52 lentiviruses and 20 µM XAV939 (Wnt/β‑catenin inhibitor), and 16HBE cells were treated with vector/oeTRIM52 lentiviruses. RPMI‑1640 medium‑treated cells served as controls. (A) The proliferation of treated‑H1975, A549 and 16HBE cells was assessed with a Cell Counting Kit‑8 assay. (B) Using flow cytom- etry, the cell cycle was evaluated after 48 h. (C) The levels of β‑catenin, PCNA, c‑Myc and Cyclin D1 proteins were also detected.

Article Snippet: Furthermore, H358 or H1299 cells were infected with RPMI-1640 medium, shNC, shTRIM52-1 or shTRIM52-4, and H1975 cells were treated with vector, oeTRIM52, Vector + 20 μM XAV939 (Wnt/β-catenin inhibitor; S1180; Selleck Chemicals, Shanghai, China) or oeTRIM52 + 20 μM XAV939.

Techniques: Plasmid Preparation, CCK-8 Assay

Journal: Oncology reports

Article Title: TRIM52 regulates the proliferation and invasiveness of lung cancer cells via the Wnt/β‑catenin pathway.

doi: 10.3892/or.2019.7110

Figure Lengend Snippet: Figure 4. Continued. (D) Invasiveness of H1975 and A549 cells was determined with a Matrigel assay. Data are expressed as the mean ± standard deviation. **P<0.01, ***P<0.001 and ****P<0.0001, oeTRIM52 or oeTRIM52 + DMSO, compared with vector or Vector + DMSO. ##P<0.01, ###P<0.001 and ####P<0.0001, oeTRIM52 + XAV939, compared with oeTRIM52 + DMSO. +P<0.05, ++P<0.01, +++P<0.001 and ++++P<0.0001, oeTRIM52 + XAV939, compared with vector + XAV939. TRIM52, tripartite motif 52; DMSO, dimethyl sulfoxide; PCNA, proliferating cell nuclear antigen.

Article Snippet: Furthermore, H358 or H1299 cells were infected with RPMI-1640 medium, shNC, shTRIM52-1 or shTRIM52-4, and H1975 cells were treated with vector, oeTRIM52, Vector + 20 μM XAV939 (Wnt/β-catenin inhibitor; S1180; Selleck Chemicals, Shanghai, China) or oeTRIM52 + 20 μM XAV939.

Techniques: Matrigel Assay, Standard Deviation, Plasmid Preparation

Journal: Oncogene

Article Title: Brg-1 targeting of novel miR550a-5p/RNF43/Wnt signaling axis regulates colorectal cancer metastasis.

doi: 10.1038/onc.2015.124

Figure Lengend Snippet: Figure 7. Brg-1 depends on RNF43/Wnt signaling mediating epithelial-to-mesenchymal transition (EMT) and metastasis in CRC. (a) Cell migration and invasion assays using transwell or Matrigel-coated transwell (left panel, representative pictures of invasion chambers; right panel, average counts from four random microscopic fields) in SW48 cells 72 h after infection with Sh-con, Sh-Brg-1 or Sh-Brg-1, as well as tranfection of pCDNA 3.1-RNF43. (b) Cell migration and invasion assays using transwell or Matrigel-coated transwell (left panel, representative pictures of invasion chambers; right panel, average counts from four random microscopic fields) in HCT116 cells 72 h after infection with Sh- con and Sh-Brg-1. (c) Luciferase assay in SW48 cells 72 h after infection with Sh-con or Sh-Brg-1 and the indicated Top-Flash reporter. (d) Cell migration and invasion assays using transwell or Matrigel-coated transwell (left panel, representative pictures of invasion chambers; right panel, average counts from four random microscopic fields) in SW48 cells 72 h after infection with Sh-con, Sh-Brg-1 or Sh-Brg-1, as well as Wnt pathway inhibitor XAV939. *Po0.05, **Po0.01, t-test.

Article Snippet: Wnt pathway inhibitor XAV939 was also purchased from Santa Cruz Biotechnology.

Techniques: Migration, Infection, Luciferase