Journal: Oncology reports

Article Title: TRIM52 regulates the proliferation and invasiveness of lung cancer cells via the Wnt/β‑catenin pathway.

doi: 10.3892/or.2019.7110

Figure Lengend Snippet: Figure 4. TRIM52 regulates proliferation, cell cycle and invasion through the Wnt/β‑catenin pathway. H1975 and A549 cells were treated with vector/oeTRIM52 lentiviruses and 20 µM XAV939 (Wnt/β‑catenin inhibitor), and 16HBE cells were treated with vector/oeTRIM52 lentiviruses. RPMI‑1640 medium‑treated cells served as controls. (A) The proliferation of treated‑H1975, A549 and 16HBE cells was assessed with a Cell Counting Kit‑8 assay. (B) Using flow cytom- etry, the cell cycle was evaluated after 48 h. (C) The levels of β‑catenin, PCNA, c‑Myc and Cyclin D1 proteins were also detected.

Article Snippet: Furthermore, H358 or H1299 cells were infected with RPMI-1640 medium, shNC, shTRIM52-1 or shTRIM52-4, and H1975 cells were treated with vector, oeTRIM52, Vector + 20 μM XAV939 (Wnt/β-catenin inhibitor; S1180; Selleck Chemicals, Shanghai, China) or oeTRIM52 + 20 μM XAV939.

Techniques: Plasmid Preparation, CCK-8 Assay

Journal: Oncology reports

Article Title: TRIM52 regulates the proliferation and invasiveness of lung cancer cells via the Wnt/β‑catenin pathway.

doi: 10.3892/or.2019.7110

Figure Lengend Snippet: Figure 4. Continued. (D) Invasiveness of H1975 and A549 cells was determined with a Matrigel assay. Data are expressed as the mean ± standard deviation. **P<0.01, ***P<0.001 and ****P<0.0001, oeTRIM52 or oeTRIM52 + DMSO, compared with vector or Vector + DMSO. ##P<0.01, ###P<0.001 and ####P<0.0001, oeTRIM52 + XAV939, compared with oeTRIM52 + DMSO. +P<0.05, ++P<0.01, +++P<0.001 and ++++P<0.0001, oeTRIM52 + XAV939, compared with vector + XAV939. TRIM52, tripartite motif 52; DMSO, dimethyl sulfoxide; PCNA, proliferating cell nuclear antigen.

Article Snippet: Furthermore, H358 or H1299 cells were infected with RPMI-1640 medium, shNC, shTRIM52-1 or shTRIM52-4, and H1975 cells were treated with vector, oeTRIM52, Vector + 20 μM XAV939 (Wnt/β-catenin inhibitor; S1180; Selleck Chemicals, Shanghai, China) or oeTRIM52 + 20 μM XAV939.

Techniques: Matrigel Assay, Standard Deviation, Plasmid Preparation

Journal: Cell Reports Methods

Article Title: Patterning ganglionic eminences in developing human brain organoids using a morphogen-gradient-inducing device

doi: 10.1016/j.crmeth.2023.100689

Figure Lengend Snippet:

Article Snippet: XAV939 [5 μM] , BioGems , 2848932-25mg.

Techniques: Recombinant, Gene Expression, Software, Imaging