Journal: Journal of neurochemistry

Article Title: Murine cathepsin D deficiency is associated with dysmyelination/myelin disruption and accumulation of cholesteryl esters in the brain.

doi: 10.1111/j.1471-4159.2009.06440.x

Figure Lengend Snippet: Fig. 5 The amount of ApoE protein is increased and that of ABCA1 decreased in the brains of Ctsd deficient mice. Aliquots of brain lysates from wild-type (+/+) and Ctsd knockout ()/)) mice (20 lg of protein lysate from each) were analyzed by western blotting using antibodies against ApoE, ABCA1, or ApoA1. ABCA1 samples were separated by 6% sodium dodecyl sulfate–polyacrylamide gel electrophoresis, ApoA1 and ApoE samples by 10-12% sodium dodecyl sulfate–poly- acrylamide gel electrophoresis. The band intensities were quantified; *p < 0.05 and **p < 0.005, n = 4 samples from two individuals per genotype, except for ApoA1 n = 5 individuals per genotype. a.u., arbitrary units; error bars, SEM.

Article Snippet: The following antibodies were used for semi-quantitative western blotting: Rabbit polyclonal antibodies against murine apolipoprotein A–I (ApoA–I) (van Haperen et al. 2000), murine ApoE (raised against purified mouse plasma ApoE protein) and ATP-binding casette transporter A1 (ABCA1) (Novus Biologicals, Littleton, CO, USA) were used for western blotting where indicated.

Techniques: Knock-Out, Western Blot, Polyacrylamide Gel Electrophoresis, SDS Page