Journal: Prostate Cancer and Prostatic Diseases

Article Title: Novel role for the transient receptor potential channel TRPM2 in prostate cancer cell proliferation

doi: 10.1038/pcan.2009.55

Figure Lengend Snippet: Selective inhibitory effect of siRNA-TRPM2 on prostate cancer cell proliferation. ( a ) Cell proliferation monitored in cell lines PC-3, DU-145, BPH-1, and RWPE-1 with WST-8 assay. The open square represents the data from untreated cells (control). The open triangle represents data from cells treated with 40 n M scrambled siRNA. The open circle represents the data from cells treated with 40 n M siRNA-TRPM2. N =3 for all groups. Error bar represents the standard deviation. * Indicates P <0.05 between siRNA-TRPM2 treatment and the two other groups. ( b ) RT–PCR showed that TRPM2 mRNA expression is inhibited by the siRNA-TRPM2 treatment in DU-145 and PC-3 2 days after the siRNA transfection. The sizes of PCR products are 293 bp and 234 bp for TRPM2 and GAPDH, respectively. ( c ) Western blot shows that TRPM2 protein expression is inhibited in PC-3 by 40 n M siRNA-TRPM2 treatment for 72 h. Repeated three times. siTRPM2: siRNA-TRPM2.

Article Snippet: Cell growth was monitored by a WST-8 cell counting kit (Alexis Biochemicals Corp., San Diego, CA, USA).

Techniques: Standard Deviation, Reverse Transcription Polymerase Chain Reaction, Expressing, Transfection, Western Blot

Journal: International Journal of Molecular and Cellular Medicine

Article Title: Comprehensive Analysis of Zinc Derivatives Pro-proliferative, Anti-Apoptotic and Antimicrobial Effect on Human Fibroblasts and Keratinocytes in a Simulated, Nutrient-deficient Environment In Vitro

doi: 10.22088/IJMCM.BUMS.9.2.165

Figure Lengend Snippet: Proliferation of human fibroblasts and keratinocytes in a simulated nutrient-deficient microenvironment in vitro under treatment with varying Zn derivative concentrations after 24 and 48 h. Fibroblasts (a, b) and keratinocytes (c, d) treated with effective Zn derivatives in concentrations of 0.001 up to 10 µg/mL for 24 and 48 h compared to an untreated control. Each experiment was performed three times, values were determined in triplicates. Data represent mean values ± SEM. * P < 0.05, ** P < 0.01, and *** P < 0.001 vs. control

Article Snippet: Cell proliferation assay To measure proliferation and viability of fibroblasts and keratinocytes, an XTT assay (Trevigen via Biozol Diagnostica GmbH, Eching, Germany) was used according to manufacturer's instruction.

Techniques: In Vitro, Control

Journal: International Journal of Molecular and Cellular Medicine

Article Title: Comprehensive Analysis of Zinc Derivatives Pro-proliferative, Anti-Apoptotic and Antimicrobial Effect on Human Fibroblasts and Keratinocytes in a Simulated, Nutrient-deficient Environment In Vitro

doi: 10.22088/IJMCM.BUMS.9.2.165

Figure Lengend Snippet: Course of fibroblast and keratinocyte proliferation over 6 days under repeated zinc supplementation in a simulated nutrient-deficient microenvironment in vitro . Fibroblasts (left column) and keratinocytes (right column) treated with zinc derivatives over the course of 6 days compared to an untreated control. Arrows indicate time-point of medium change (nutrient-deficient) and repeated zinc supplementation. Each experiment was performed three times, values were determined in triplicates. Data represent mean values ± SEM. Symbols represent significant changes in proliferation compared to the untreated control (# for 10 µg/mL; + for 0.1 µg/mL; * for 0.01 µg/mL, and § for 0.001 µg/mL). One symbol equals P < 0.05, two P < 0.01 and three P < 0.001 vs. control

Article Snippet: Cell proliferation assay To measure proliferation and viability of fibroblasts and keratinocytes, an XTT assay (Trevigen via Biozol Diagnostica GmbH, Eching, Germany) was used according to manufacturer's instruction.

Techniques: In Vitro, Control

Journal: International Journal of Molecular and Cellular Medicine

Article Title: Comprehensive Analysis of Zinc Derivatives Pro-proliferative, Anti-Apoptotic and Antimicrobial Effect on Human Fibroblasts and Keratinocytes in a Simulated, Nutrient-deficient Environment In Vitro

doi: 10.22088/IJMCM.BUMS.9.2.165

Figure Lengend Snippet: Gene expression of proliferation- and apoptosis-associated genes of human fibroblasts and keratinocytes. Fibroblasts and keratinocytes cultured in nutrient-deficient media and treated with different concentrations of Zn derivatives for 48 and 24 h, respectively. qRT-PCR measurements of proliferation-associated genes E2F1 , PCNA and apoptosis gene TP53 mRNA levels were performed in cells treated with Zn derivatives as well as untreated cells and normalized to GAPDH levels. Each experiment was performed three times, values were determined in triplicates. Data represent means ± SEM. * P < 0.05, ** P < 0.01, and *** P < 0.001 vs. control

Article Snippet: Cell proliferation assay To measure proliferation and viability of fibroblasts and keratinocytes, an XTT assay (Trevigen via Biozol Diagnostica GmbH, Eching, Germany) was used according to manufacturer's instruction.

Techniques: Gene Expression, Cell Culture, Quantitative RT-PCR, Control