Journal: BMC Pulmonary Medicine

Article Title: Astragaloside IV restrains pyroptosis and fibrotic development of pulmonary artery smooth muscle cells to ameliorate pulmonary artery hypertension through the PHD2/HIF1α signaling pathway

doi: 10.1186/s12890-023-02660-9

Figure Lengend Snippet: AS-IV reduced pyroptosis and fibrosis development in hypoxia-treated PASMCs. PASMCs were divided into 3 groups: Nor, Hyp and AS, cultured with normoxia or hypoxia, and treated with or without AS-IV. A - D , Cropped blots of GSDMD-N, NLRP3, cleaved Caspase-1, Fibronectin and Collagen1 and quantification of grayscale value (the samples derived from the same experiment and that gels/blots were processed in parallel). E , IL-1β and IL-18 levels in cell supernatant were measured by ELISA. F , Cell death was detected by PI/Hoechst double fluorescent staining. Scale bar = 50 μm. G, LDH activity was detected by a LDH release agent. H - I , Proteins related to extracellular matrix metabolism, including MMP2/9 and TIMP4 were detected by ELISA method. * P <0.05, ** P <0.01, *** P <0.001, comparison with Nor group. # P <0.05, ## P <0.01, ### P <0.001, comparison with Hyp group

Article Snippet: The nuclei were counterstained with Hoechst 33,258 (Abcam, #ab228550), and the image was visualized under a microscope and analyzed by the image J software.

Techniques: Cell Culture, Derivative Assay, Enzyme-linked Immunosorbent Assay, Staining, Activity Assay, Comparison

Journal: BMC Pulmonary Medicine

Article Title: Astragaloside IV restrains pyroptosis and fibrotic development of pulmonary artery smooth muscle cells to ameliorate pulmonary artery hypertension through the PHD2/HIF1α signaling pathway

doi: 10.1186/s12890-023-02660-9

Figure Lengend Snippet: AS-IV depressed pyroptosis and the production of fibrosis markers induced by hypoxia in PASMCs through the PHD2-HIF1α axis. A - D , PASMCs were divided into 5 groups: Nor, Hyp, AS, AS + si-PHD2 and LW6. Cropped blots of GSDMD-N, NLRP3, cleaved Caspase-1, Fibronectin and Collagen1 (the samples derived from the same experiment and that gels/blots were processed in parallel), and quantitation of the pyroptosis markers and the fibrosis markers. E , Cell death was detected by PI/Hoechst double fluorescent staining. Scale bar = 50 μm. F , IL-1β and IL-18 levels in cell supernatant were measured by ELISA. G, LDH activity was detected by a LDH release agent. H - I , Proteins of MMP2/9 and TIMP4 were detected by ELISA method. * P <0.05, ** P <0.01, *** P <0.001, comparison with Nor group. # P <0.05, ## P <0.01, ### P <0.001, comparison with Hyp group. ^ P <0.05, ^^ P <0.01, ^^^ P <0.001, comparison with AS group

Article Snippet: The nuclei were counterstained with Hoechst 33,258 (Abcam, #ab228550), and the image was visualized under a microscope and analyzed by the image J software.

Techniques: Derivative Assay, Quantitation Assay, Staining, Enzyme-linked Immunosorbent Assay, Activity Assay, Comparison

Journal: International Journal of Molecular Sciences

Article Title: Novel Bioactive Resin Coating with Calcium Phosphate Nanoparticles for Antibacterial and Remineralization Abilities to Combat Tooth Root Caries

doi: 10.3390/ijms26062490

Figure Lengend Snippet: Metabolic activity of the biofilm assessed using the MTT assay for commercial controls 1 and 2, experimental control, and experimental groups (mean ± SD; n = 6). Presence of 5% DMAHDM diminished the metabolic activity of the S. mutans by ≈96.57%. Groups with different letters indicate statistically significant differences ( p < 0.05).

Article Snippet: The metabolic activity of the biofilm was assessed using a 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay (WST-8, Selleckchem, Houston, TX, USA).

Techniques: Activity Assay, MTT Assay, Control

Journal: Bioengineering

Article Title: Focused Low-Intensity Pulsed Ultrasound (FLIPUS) Mitigates Apoptosis of MLO-Y4 Osteocyte-like Cells

doi: 10.3390/bioengineering10030387

Figure Lengend Snippet: FLIPUS enhances the metabolic activity of starved MLO-Y4 osteocytes, measured by WST-8 assay. FI to NC stands for fold induction over negative control, samples which did not receive stimulation with ultrasound. Each data point was the result of N = 3 and n = 3, where N and n are biological trials and technical replicates, respectively. ** and *** represent p ≤ 0.01 and p ≤ 0.001, respectively.

Article Snippet: WST-8 solution (BIOZOL DV GmbH, Eching, Germany) then was added to each well in a 1:10 ratio, and the cells were cultured in the incubator for another 30 min. Absorbance was measured at 450 nm with reference to 620 nm on a BMG LabTech FLUORstar OPTIMA reader (2015 series, Ortenberg, Germany).

Techniques: Activity Assay, Negative Control