Journal: Nature Communications

Article Title: Ion suppression correction and normalization for non-targeted metabolomics

doi: 10.1038/s41467-025-56646-8

Figure Lengend Snippet: a The IROA ion suppression correction workflow. b Number of MSTUS peaks detected across analytical conditions. c–k Raw MSTUS-12C (blue lines) and suppression-corrected MSTUS-12C (red lines) values are shown for: c HILIC positive mode, uncleaned source; d HILIC positive mode, clean source; e HILIC negative mode, uncleaned source; f HILIC negative mode, clean source; g RPLC positive mode, uncleaned source; h RPLC positive mode, clean source; ( i ) RPLC negative mode, uncleaned source; j RPLC negative mode, clean source; and k IC negative mode, cleaned source. l Ratio of raw MSTUS-12C to suppression-corrected MSTUS-12C peak intensity across chromatographic methods and experimental conditions. m Raw and suppression-corrected phenylalanine values in RPLC positive ionization mode with cleaned source. n Raw and suppression-corrected pyroglutamylglycine values in IC negative ionization mode. o Identified chemical composition in entire RPLC clean dataset, as an example. p Kohonen Self Organizing Maps (SOM) show suppression patterns in the RPLC Clean raw dataset for all 539 compounds. q Density map shows compounds associated with each of the patterns discovered in ( o ). r Raw MSTUS-12C (blue lines) and suppression-corrected MSTUS-12C (red lines) values are shown for RPLC positive mode and RPLC negative mode ( s ) for urine. t Ratio of raw MSTUS-12C to suppression-corrected 12 C peak intensity for urine matrix in positive and negative ion modes. u Plasma and urine MSTUS-12C signals for 4 common metabolites before and after suppression correction. 12C SC = 12C suppression corrected MSTUS; 13C raw = 13C raw MSTUS. Colors represent percent peak intensity as indicated by the color bar. Source data are provided as a Source Data file. Data are shown as mean ± s.d. ( c – n , r , s ).

Article Snippet: Using the IROA Workflow, analysts can err on the side of injecting larger sample volumes to ensure robust measurement of low-abundance analytes while simultaneously performing ion suppression correction to achieve more accurate results.

Techniques: Hydrophilic Interaction Liquid Chromatography

Journal: Nature Communications

Article Title: Ion suppression correction and normalization for non-targeted metabolomics

doi: 10.1038/s41467-025-56646-8

Figure Lengend Snippet: a Optimization of cancer cell count for the IROA-IS ion suppression correction workflow. Raw MSTUS-12C (blue lines), suppression-corrected MSTUS-12C (red lines), and DUAL-MSTUS normalized (green lines) values are shown for: b RPLC positive mode, cleaned source; and c RPLC negative mode, clean source. d Optimization of IROA-IS during extraction and during reconstitution for ion suppression correction workflow. e Identified chemical composition in OVCAR-8 cell using IROA-IS in extraction solvent for entire RPLC clean dataset by both positive and negative mode. f Identified chemical composition in OVCAR-8 cell using IROA-IS as reconstitute solvent for entire RPLC clean dataset by both positive and negative mode. g Identified chemical composition in OVCAR-4 cell using IROA-IS in extraction solvent for entire RPLC clean dataset by both positive and negative mode. h Identified chemical composition in OVCAR-4 cell using IROA-IS as reconstitute solvent for entire RPLC clean dataset by both positive and negative mode. i Percent coefficient of variation (%CV) for raw, suppression-corrected, and normalized data from OVCAR-8 cell using IROA-IS in extraction solvent for entire RPLC clean dataset by both positive and negative mode. j Percent coefficient of variation (%CV) for raw, suppression-corrected, and normalized data from OVCAR-8 cell using IROA-IS as reconstitute solvent for entire RPLC clean dataset by both positive and negative mode. k Percent coefficient of variation (%CV) for raw, suppression-corrected, and normalized data from OVCAR-4 cell using IROA-IS in extraction solvent for entire RPLC clean dataset by both positive and negative mode. l Percent coefficient of variation (%CV) for raw, suppression-corrected, and normalized data from OVCAR-4 cell using IROA-IS as reconstitute solvent for entire RPLC clean dataset by both positive and negative mode. Percent coefficient of variation (%CV) for non-IROA-driven raw, and IROA-driven raw, suppression-corrected, and normalized data from OVCAR-4 ( m ) and OVCAR-8 ( n ) cell lines, respectively, for entire RPLC clean dataset by both positive and negative mode. Colors represent percent peak intensity as indicated by the color bar. Source data are provided as a Source Data file. Data are shown as mean ± s.d. ( b , c ).

Article Snippet: Using the IROA Workflow, analysts can err on the side of injecting larger sample volumes to ensure robust measurement of low-abundance analytes while simultaneously performing ion suppression correction to achieve more accurate results.

Techniques: Cell Counting, Extraction, Solvent

Journal: Clinical Ophthalmology (Auckland, N.Z.)

Article Title: Time Savings Using a Digital Workflow versus a Conventional for Intraocular Lens Implantation in a Corporate Chain Hospital Setting

doi: 10.2147/OPTH.S439930

Figure Lengend Snippet: Time assessments for digital cataract workflow and existing conventional workflow. *Time 1 was calculated as the sum of the mean times recorded for each step: (IOL Master) + (Pentacam) + (OCT) + (endothelial cell count). **Time 3: Digital transfer step not applicable in digital cataract workflow as it is automated. # Times 4 and 5: IOL axis marking is not applicable in the digital cataract workflow as it is done via the FORUM ® platform/CALLISTO eye.

Article Snippet: In conclusion, significant time savings at each step of cataract surgery planning were observed with the Zeiss digital cataract workflow compared with the existing conventional workflow.

Techniques: Cell Counting

Journal: Clinical Ophthalmology (Auckland, N.Z.)

Article Title: Time Savings Using a Digital Workflow versus a Conventional for Intraocular Lens Implantation in a Corporate Chain Hospital Setting

doi: 10.2147/OPTH.S439930

Figure Lengend Snippet: Time Measurements at Various Steps in Site’s Existing Conventional and Digital Cataract Workflow

Article Snippet: In conclusion, significant time savings at each step of cataract surgery planning were observed with the Zeiss digital cataract workflow compared with the existing conventional workflow.

Techniques: Cell Counting

Journal: Clinical Ophthalmology (Auckland, N.Z.)

Article Title: Time Savings Using a Digital Workflow versus a Conventional for Intraocular Lens Implantation in a Corporate Chain Hospital Setting

doi: 10.2147/OPTH.S439930

Figure Lengend Snippet: Inter observer variability in the digital cataract workflow versus existing conventional workflow for preoperative assessments.

Article Snippet: In conclusion, significant time savings at each step of cataract surgery planning were observed with the Zeiss digital cataract workflow compared with the existing conventional workflow.

Techniques:

Journal: Clinical Ophthalmology (Auckland, N.Z.)

Article Title: Time Savings Using a Digital Workflow versus a Conventional for Intraocular Lens Implantation in a Corporate Chain Hospital Setting

doi: 10.2147/OPTH.S439930

Figure Lengend Snippet: Overall time savings in digital cataract workflow versus existing conventional workflow.

Article Snippet: In conclusion, significant time savings at each step of cataract surgery planning were observed with the Zeiss digital cataract workflow compared with the existing conventional workflow.

Techniques:

Journal: bioRxiv

Article Title: A Cryo-/Liquid Phase Correlative Light Electron Microscopy Workflow to Visualize Crystallization Processes in Graphene Liquid Cells

doi: 10.1101/2023.05.08.539575

Figure Lengend Snippet: Schematic overview of the cryo-/LP-CLEM workflow. Graphene liquid cell preparation is completely automated. The machine etches away the copper (7°C), transfers the graphene to a TEM grid via loop-assisted transfer and seals the GLCs by blotting away excess liquid. The reaction starts once the liquid pockets are sealed. Live fluorescence microscopy can be used to determine not only the location, but the specific time to image at high resolution, at which point the process is arrested by rapid vitrification. ROIs are located using cryo-fluorescence microscopy and hereafter they are imaged at nanometer resolution (cryo-TEM). The process is reinitiated inside the microscope by heating-up the grid using a cryo-holder, and the reaction dynamics are directly recorded (LP-TEM). To confirm that GLCs remain intact and retain liquid after TEM observation, fluorescence microscopy is used.

Article Snippet: After vitrification, the TEM grids were loaded into a universal TEM cryo-holder (349559-8100-010) using the ZEISS Correlative Cryo Workflow solution, which fit into the PrepDek® (PP3010Z, Quorum technologies, Laughton, UK).

Techniques: Fluorescence, Microscopy

Journal: bioRxiv

Article Title: A Cryo-/Liquid Phase Correlative Light Electron Microscopy Workflow to Visualize Crystallization Processes in Graphene Liquid Cells

doi: 10.1101/2023.05.08.539575

Figure Lengend Snippet: Cryo-/LP-CLEM workflow to visualize crystallization processes inside a GLC. a) TEM overview imaged two days after thawing, overlaid with live-FM (green) to indicate the GLCs. Insert shows a high magnification LP-TEM image of the GLC in the blue box. b) SAED pattern taken at the position indicated by the black dashed circle in . Inner ring in the DP shows the contribution of the [100] plane on NaCl and outer ring the contribution of the graphene. c) Enlargement of the area marked by the orange box in were multiple GLCs are present (green, yellow and purple boxes) and a crystal that is not encapsulated by graphene (red box). D-f) Large crystal inside a GLC imaged at multiple time points after thawing (3d: 2 days; 3e: 5 days; 3f: 7 days) shows morphological changes. Graphene wrinkles (close yellow arrow), outline of the GLC (dotted yellow line) and the intensity gradient of the liquid surrounding the crystal (open yellow arrow) are visible at all time points. g) Two different but interconnected GLCs imaged two days after thawing (open and closed purple arrows). h) Condensation (solid arrow), and phase transformation (open purple arrow) observed within the pockets five days after thawing. I) Cubic NaCl crystal resulting from an Ostwald ripening process observed seven days after thawing j) Liquid pockets (green dashed circles) containing an amorphous-like phase. k) Nucleation within the amorphous phase contained in the GLCs (green striped circles). l) Dissolution of small crystals (top circle) and further crystallization (bottom circle) inside the GLCs. m) Crystal not encapsulated by graphene outlined by the red dotted line shows only minimum morphological changes five (3n) and seven (3o) days after thawing. Accumulative dose: a) 0.75e - /Å ; c, d, g, j, m) 0.15 e - /Å ; e, h, k, n) 0.30 e - /Å ; f, i, l, o) 0.45 e - /Å

Article Snippet: After vitrification, the TEM grids were loaded into a universal TEM cryo-holder (349559-8100-010) using the ZEISS Correlative Cryo Workflow solution, which fit into the PrepDek® (PP3010Z, Quorum technologies, Laughton, UK).

Techniques: Crystallization Assay, Transformation Assay