Journal: PLoS ONE

Article Title: The molecular connection of histopathological heterogeneity in hepatocellular carcinoma: A role of Wnt and Hedgehog signaling pathways

doi: 10.1371/journal.pone.0208194

Figure Lengend Snippet: Primer set of various genes adopted in RT-PCR.

Article Snippet: Primary antibodies β-Catenin (Cat: SC-7963), Wnt3 (Cat: ab50341), PCNA (Cat: SC-56), Gli1 (Cat: SC-20687), Gli2 (Cat: SC-28674), sFRP1 (Cat: ab126613), MMP9 (Cat: SC-393859), GSK3β (Cat: SC-9166), Shh (Cat: SC-9024), CyclinD1(Cat:SC-20044), CyclinB1(Cat:SC-752) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA).

Techniques:

Journal: PLoS ONE

Article Title: The molecular connection of histopathological heterogeneity in hepatocellular carcinoma: A role of Wnt and Hedgehog signaling pathways

doi: 10.1371/journal.pone.0208194

Figure Lengend Snippet: Enhanced Wnt and Hh signaling correlates with moderately differentiated HCC. (A) H and E stained liver sections of control and promotion group (group2) animals showing moderately differentiated, thick trabecular pattern, followed by photomicraphs showing IHC staining of Wnt and Hh pathway molecules at TE and TL phases of promotion stage. The corresponding IHC staining intensity graph is shown in the side panel of figures.Y-axis represents labeling index (%) visual score in each intensity graph. (B) Increasing mRNA expression level of Wnt and Hh pathway molecules of control and group2 animals at TE and TL phases of promotion stage. (C) The figure represents fold increase in relative mRNA expression, calculated with respect to GAPDH for Shh, Ptch1, Gli1, Wnt3 β-catenin in control and group2 animals at TE and TL phases of promotion stage (D) Analysis of Wnt and Hh pathway molecules in rat tissue lysates at TE and TL phases of promotion stage by ELISA. Data presented are representative of three independent experiments performed in triplicates and expressed as Mean±S.D. ** and *** differs significantly at p <0.005 and <0.0005.

Article Snippet: Primary antibodies β-Catenin (Cat: SC-7963), Wnt3 (Cat: ab50341), PCNA (Cat: SC-56), Gli1 (Cat: SC-20687), Gli2 (Cat: SC-28674), sFRP1 (Cat: ab126613), MMP9 (Cat: SC-393859), GSK3β (Cat: SC-9166), Shh (Cat: SC-9024), CyclinD1(Cat:SC-20044), CyclinB1(Cat:SC-752) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA).

Techniques: Staining, Control, Immunohistochemistry, Labeling, Expressing, Enzyme-linked Immunosorbent Assay

Journal: Clinical science (London, England : 1979)

Article Title: Restoration of the gut barrier integrity and restructuring of the gut microbiome in aging by angiotensin-(1–7)

doi: 10.1042/CS20220904

Figure Lengend Snippet: List of antibodies used for western blotting and immunohistochemistry

Article Snippet: Antibody Catalog number Vendor Concentration Claudin 1 (Host - Ms) 37-4900 Thermofisher 1:100 Occludin (Host -Rb) 40-4700 Thermofisher 1:100 Lgr5 (Host – Ms) MA5-25644 Thermofisher 1:100 Olfm4 (Host – Rb) PA5-115687 Thermofisher 1:50 F4/80 (Host - Rat) 14-4801-82 Invitrogen 1:50 CD11b (Host - Rb) ab133357 Abcam 1:4000 633 Goat Anti Rb 20122 Biotium 1:250 633 Goat Anti Ms 20010 Biotium 1:250 488 Goat Anti Rat A11006 Invitrogen 1:100 DAPI 40043 Biotium - ACE MA5- 32741 Thermofisher 1: 1000 ACE-2 MA5- 32307 Thermofisher 1: 1000 MAS1 (G-1) Sc- 390453 Santacruz 1: 1000 AT-1 (G-3) Sc- 515884 Santacruz 1: 1000 AT-2 MA5- 32293 Thermofisher 1: 1000 Wnt3a TA801736S Origene 1:1000 (western blot) and 1:100 (IHC) Open in a separate window List of antibodies used for western blotting and immunohistochemistry.

Techniques: Western Blot, Concentration Assay

Journal: Clinical science (London, England : 1979)

Article Title: Restoration of the gut barrier integrity and restructuring of the gut microbiome in aging by angiotensin-(1–7)

doi: 10.1042/CS20220904

Figure Lengend Snippet: (A) Shown were representative fluorescence images of Wnt3a immunohistochemistry with nuclear counter stain DAPI in colon sections derived from young, old and Ang-(1–7)-treated mice. (B) The number of Wnt3a-positive cells/villus was lower in the sections of old colon (n = 28 villi/5 mice/group) compared with the young (****P<0.0001, n = 30 villi/5 mice/group), which was increased in the Ang-(1–7)-treated old group (***P<0.001, n = 25 villi/5 mice/group). (C) Shown were representative Western blots of Wnt3a in the lysates of colon tissue or ex vivo-cultured organoids from different treatment groups. (D) Wnt3a protein levels were lower in the colons of old compared with the young (*P<0.05, n = 5 mice/group), which was increased in the colons derived from Ang-(1–7)-treated old group (*P<0.05, n = 5 mice/group). In the ex vivo-cultured organoids, decreased Wnt3a levels in the organoids from old compared with that derived from young colons, which was increased by treatment with Ang-(1–7) (*P<0.05, n = 5 mice/group). Data sets were analyzed by Kruskall–Wallis test followed by Dunn’s test.

Article Snippet: Antibody Catalog number Vendor Concentration Claudin 1 (Host - Ms) 37-4900 Thermofisher 1:100 Occludin (Host -Rb) 40-4700 Thermofisher 1:100 Lgr5 (Host – Ms) MA5-25644 Thermofisher 1:100 Olfm4 (Host – Rb) PA5-115687 Thermofisher 1:50 F4/80 (Host - Rat) 14-4801-82 Invitrogen 1:50 CD11b (Host - Rb) ab133357 Abcam 1:4000 633 Goat Anti Rb 20122 Biotium 1:250 633 Goat Anti Ms 20010 Biotium 1:250 488 Goat Anti Rat A11006 Invitrogen 1:100 DAPI 40043 Biotium - ACE MA5- 32741 Thermofisher 1: 1000 ACE-2 MA5- 32307 Thermofisher 1: 1000 MAS1 (G-1) Sc- 390453 Santacruz 1: 1000 AT-1 (G-3) Sc- 515884 Santacruz 1: 1000 AT-2 MA5- 32293 Thermofisher 1: 1000 Wnt3a TA801736S Origene 1:1000 (western blot) and 1:100 (IHC) Open in a separate window List of antibodies used for western blotting and immunohistochemistry.

Techniques: Fluorescence, Immunohistochemistry, Staining, Derivative Assay, Western Blot, Ex Vivo, Cell Culture

Journal: Cancer Research Communications

Article Title: Suppressed Cellular Senescence Mediated by T-box3 in Aged Gastric Epithelial Cells may Contribute to Aging-related Carcinogenesis

doi: 10.1158/2767-9764.CRC-22-0084

Figure Lengend Snippet: Primer sets used for Q-PCR in this study

Article Snippet: Fzd2 , Mm02524776_s1 , Wnt3 , Mm00437336_m1.

Techniques: