wga Search Results


wheat germ agglutinin  (Vector Laboratories)
96
Vector Laboratories wheat germ agglutinin
Wheat Germ Agglutinin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
wheat germ agglutinin - by Bioz Stars, 2026-04
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methylated human dna  (Zymo Research)
94
Zymo Research methylated human dna
Histone methylation and CpG <t>DNA</t> methylation of the Notch-1 promoter are increased in T cells from SLE patients. A, histone H3K27 methylation was analyzed in total T cells from three age-, gender-, and ethnicity-matched control (CON)/SLE pairs by ChIP assays. Dotted lines associate matched samples. A region of interest within the human Notch-1 promoter (harboring a putative CRE) was amplified by qPCR, and the proportion of immunoprecipitated DNA was calculated as relative to the non-immunoprecipitated input DNA in each sample. Subsequently, the ratio of relative expression was calculated between each SLE patient and the corresponding control individual. Horizontal lines represent mean values. B, the dotted line represents the H3K27 methylation status in control T cells, for each of which was set to 1.0. Changes in the methylation status in the matched SLE patient are given in the bar diagram (mean ± S.D.). C, total T cells from 15 healthy controls (light gray bar), 6 inactive SLE patients (dark gray bar), and 12 active SLE patients (black bar) were subjected <t>to</t> <t>methylated</t> DNA immunoprecipitation. The percentage of methylated DNA is given as mean ± S.D.
Methylated Human Dna, supplied by Zymo Research, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/methylated human dna/product/Zymo Research
Average 94 stars, based on 1 article reviews
methylated human dna - by Bioz Stars, 2026-04
94/100 stars
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rhodamine conjugated 135 6 lectin  (Vector Laboratories)
94
Vector Laboratories rhodamine conjugated 135 6 lectin
Histone methylation and CpG <t>DNA</t> methylation of the Notch-1 promoter are increased in T cells from SLE patients. A, histone H3K27 methylation was analyzed in total T cells from three age-, gender-, and ethnicity-matched control (CON)/SLE pairs by ChIP assays. Dotted lines associate matched samples. A region of interest within the human Notch-1 promoter (harboring a putative CRE) was amplified by qPCR, and the proportion of immunoprecipitated DNA was calculated as relative to the non-immunoprecipitated input DNA in each sample. Subsequently, the ratio of relative expression was calculated between each SLE patient and the corresponding control individual. Horizontal lines represent mean values. B, the dotted line represents the H3K27 methylation status in control T cells, for each of which was set to 1.0. Changes in the methylation status in the matched SLE patient are given in the bar diagram (mean ± S.D.). C, total T cells from 15 healthy controls (light gray bar), 6 inactive SLE patients (dark gray bar), and 12 active SLE patients (black bar) were subjected <t>to</t> <t>methylated</t> DNA immunoprecipitation. The percentage of methylated DNA is given as mean ± S.D.
Rhodamine Conjugated 135 6 Lectin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rhodamine conjugated 135 6 lectin/product/Vector Laboratories
Average 94 stars, based on 1 article reviews
rhodamine conjugated 135 6 lectin - by Bioz Stars, 2026-04
94/100 stars
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biotinylated wheat germ agglutinin  (Vector Laboratories)
94
Vector Laboratories biotinylated wheat germ agglutinin
Histone methylation and CpG <t>DNA</t> methylation of the Notch-1 promoter are increased in T cells from SLE patients. A, histone H3K27 methylation was analyzed in total T cells from three age-, gender-, and ethnicity-matched control (CON)/SLE pairs by ChIP assays. Dotted lines associate matched samples. A region of interest within the human Notch-1 promoter (harboring a putative CRE) was amplified by qPCR, and the proportion of immunoprecipitated DNA was calculated as relative to the non-immunoprecipitated input DNA in each sample. Subsequently, the ratio of relative expression was calculated between each SLE patient and the corresponding control individual. Horizontal lines represent mean values. B, the dotted line represents the H3K27 methylation status in control T cells, for each of which was set to 1.0. Changes in the methylation status in the matched SLE patient are given in the bar diagram (mean ± S.D.). C, total T cells from 15 healthy controls (light gray bar), 6 inactive SLE patients (dark gray bar), and 12 active SLE patients (black bar) were subjected <t>to</t> <t>methylated</t> DNA immunoprecipitation. The percentage of methylated DNA is given as mean ± S.D.
Biotinylated Wheat Germ Agglutinin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/biotinylated wheat germ agglutinin/product/Vector Laboratories
Average 94 stars, based on 1 article reviews
biotinylated wheat germ agglutinin - by Bioz Stars, 2026-04
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rhodamine conjugated wheat germ agglutinin wga  (Vector Laboratories)
93
Vector Laboratories rhodamine conjugated wheat germ agglutinin wga
Histone methylation and CpG <t>DNA</t> methylation of the Notch-1 promoter are increased in T cells from SLE patients. A, histone H3K27 methylation was analyzed in total T cells from three age-, gender-, and ethnicity-matched control (CON)/SLE pairs by ChIP assays. Dotted lines associate matched samples. A region of interest within the human Notch-1 promoter (harboring a putative CRE) was amplified by qPCR, and the proportion of immunoprecipitated DNA was calculated as relative to the non-immunoprecipitated input DNA in each sample. Subsequently, the ratio of relative expression was calculated between each SLE patient and the corresponding control individual. Horizontal lines represent mean values. B, the dotted line represents the H3K27 methylation status in control T cells, for each of which was set to 1.0. Changes in the methylation status in the matched SLE patient are given in the bar diagram (mean ± S.D.). C, total T cells from 15 healthy controls (light gray bar), 6 inactive SLE patients (dark gray bar), and 12 active SLE patients (black bar) were subjected <t>to</t> <t>methylated</t> DNA immunoprecipitation. The percentage of methylated DNA is given as mean ± S.D.
Rhodamine Conjugated Wheat Germ Agglutinin Wga, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rhodamine conjugated wheat germ agglutinin wga/product/Vector Laboratories
Average 93 stars, based on 1 article reviews
rhodamine conjugated wheat germ agglutinin wga - by Bioz Stars, 2026-04
93/100 stars
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fluorescein conjugated wheat germ agglutinin  (Vector Laboratories)
95
Vector Laboratories fluorescein conjugated wheat germ agglutinin
Histone methylation and CpG <t>DNA</t> methylation of the Notch-1 promoter are increased in T cells from SLE patients. A, histone H3K27 methylation was analyzed in total T cells from three age-, gender-, and ethnicity-matched control (CON)/SLE pairs by ChIP assays. Dotted lines associate matched samples. A region of interest within the human Notch-1 promoter (harboring a putative CRE) was amplified by qPCR, and the proportion of immunoprecipitated DNA was calculated as relative to the non-immunoprecipitated input DNA in each sample. Subsequently, the ratio of relative expression was calculated between each SLE patient and the corresponding control individual. Horizontal lines represent mean values. B, the dotted line represents the H3K27 methylation status in control T cells, for each of which was set to 1.0. Changes in the methylation status in the matched SLE patient are given in the bar diagram (mean ± S.D.). C, total T cells from 15 healthy controls (light gray bar), 6 inactive SLE patients (dark gray bar), and 12 active SLE patients (black bar) were subjected <t>to</t> <t>methylated</t> DNA immunoprecipitation. The percentage of methylated DNA is given as mean ± S.D.
Fluorescein Conjugated Wheat Germ Agglutinin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fluorescein conjugated wheat germ agglutinin/product/Vector Laboratories
Average 95 stars, based on 1 article reviews
fluorescein conjugated wheat germ agglutinin - by Bioz Stars, 2026-04
95/100 stars
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single cell wga kit  (Vazyme Biotech Co)
94
Vazyme Biotech Co single cell wga kit
Histone methylation and CpG <t>DNA</t> methylation of the Notch-1 promoter are increased in T cells from SLE patients. A, histone H3K27 methylation was analyzed in total T cells from three age-, gender-, and ethnicity-matched control (CON)/SLE pairs by ChIP assays. Dotted lines associate matched samples. A region of interest within the human Notch-1 promoter (harboring a putative CRE) was amplified by qPCR, and the proportion of immunoprecipitated DNA was calculated as relative to the non-immunoprecipitated input DNA in each sample. Subsequently, the ratio of relative expression was calculated between each SLE patient and the corresponding control individual. Horizontal lines represent mean values. B, the dotted line represents the H3K27 methylation status in control T cells, for each of which was set to 1.0. Changes in the methylation status in the matched SLE patient are given in the bar diagram (mean ± S.D.). C, total T cells from 15 healthy controls (light gray bar), 6 inactive SLE patients (dark gray bar), and 12 active SLE patients (black bar) were subjected <t>to</t> <t>methylated</t> DNA immunoprecipitation. The percentage of methylated DNA is given as mean ± S.D.
Single Cell Wga Kit, supplied by Vazyme Biotech Co, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/single cell wga kit/product/Vazyme Biotech Co
Average 94 stars, based on 1 article reviews
single cell wga kit - by Bioz Stars, 2026-04
94/100 stars
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agarose wheat germ agglutinin  (Vector Laboratories)
94
Vector Laboratories agarose wheat germ agglutinin
Histone methylation and CpG <t>DNA</t> methylation of the Notch-1 promoter are increased in T cells from SLE patients. A, histone H3K27 methylation was analyzed in total T cells from three age-, gender-, and ethnicity-matched control (CON)/SLE pairs by ChIP assays. Dotted lines associate matched samples. A region of interest within the human Notch-1 promoter (harboring a putative CRE) was amplified by qPCR, and the proportion of immunoprecipitated DNA was calculated as relative to the non-immunoprecipitated input DNA in each sample. Subsequently, the ratio of relative expression was calculated between each SLE patient and the corresponding control individual. Horizontal lines represent mean values. B, the dotted line represents the H3K27 methylation status in control T cells, for each of which was set to 1.0. Changes in the methylation status in the matched SLE patient are given in the bar diagram (mean ± S.D.). C, total T cells from 15 healthy controls (light gray bar), 6 inactive SLE patients (dark gray bar), and 12 active SLE patients (black bar) were subjected <t>to</t> <t>methylated</t> DNA immunoprecipitation. The percentage of methylated DNA is given as mean ± S.D.
Agarose Wheat Germ Agglutinin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/agarose wheat germ agglutinin/product/Vector Laboratories
Average 94 stars, based on 1 article reviews
agarose wheat germ agglutinin - by Bioz Stars, 2026-04
94/100 stars
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succinylated vector laboratories cat  (Vector Laboratories)
93
Vector Laboratories succinylated vector laboratories cat
Histone methylation and CpG <t>DNA</t> methylation of the Notch-1 promoter are increased in T cells from SLE patients. A, histone H3K27 methylation was analyzed in total T cells from three age-, gender-, and ethnicity-matched control (CON)/SLE pairs by ChIP assays. Dotted lines associate matched samples. A region of interest within the human Notch-1 promoter (harboring a putative CRE) was amplified by qPCR, and the proportion of immunoprecipitated DNA was calculated as relative to the non-immunoprecipitated input DNA in each sample. Subsequently, the ratio of relative expression was calculated between each SLE patient and the corresponding control individual. Horizontal lines represent mean values. B, the dotted line represents the H3K27 methylation status in control T cells, for each of which was set to 1.0. Changes in the methylation status in the matched SLE patient are given in the bar diagram (mean ± S.D.). C, total T cells from 15 healthy controls (light gray bar), 6 inactive SLE patients (dark gray bar), and 12 active SLE patients (black bar) were subjected <t>to</t> <t>methylated</t> DNA immunoprecipitation. The percentage of methylated DNA is given as mean ± S.D.
Succinylated Vector Laboratories Cat, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/succinylated vector laboratories cat/product/Vector Laboratories
Average 93 stars, based on 1 article reviews
succinylated vector laboratories cat - by Bioz Stars, 2026-04
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picoplex single cell wga kit v3  (TaKaRa)
94
TaKaRa picoplex single cell wga kit v3
Histone methylation and CpG <t>DNA</t> methylation of the Notch-1 promoter are increased in T cells from SLE patients. A, histone H3K27 methylation was analyzed in total T cells from three age-, gender-, and ethnicity-matched control (CON)/SLE pairs by ChIP assays. Dotted lines associate matched samples. A region of interest within the human Notch-1 promoter (harboring a putative CRE) was amplified by qPCR, and the proportion of immunoprecipitated DNA was calculated as relative to the non-immunoprecipitated input DNA in each sample. Subsequently, the ratio of relative expression was calculated between each SLE patient and the corresponding control individual. Horizontal lines represent mean values. B, the dotted line represents the H3K27 methylation status in control T cells, for each of which was set to 1.0. Changes in the methylation status in the matched SLE patient are given in the bar diagram (mean ± S.D.). C, total T cells from 15 healthy controls (light gray bar), 6 inactive SLE patients (dark gray bar), and 12 active SLE patients (black bar) were subjected <t>to</t> <t>methylated</t> DNA immunoprecipitation. The percentage of methylated DNA is given as mean ± S.D.
Picoplex Single Cell Wga Kit V3, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 1 article reviews
picoplex single cell wga kit v3 - by Bioz Stars, 2026-04
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lectin swga  (Vector Laboratories)
94
Vector Laboratories lectin swga
Histone methylation and CpG <t>DNA</t> methylation of the Notch-1 promoter are increased in T cells from SLE patients. A, histone H3K27 methylation was analyzed in total T cells from three age-, gender-, and ethnicity-matched control (CON)/SLE pairs by ChIP assays. Dotted lines associate matched samples. A region of interest within the human Notch-1 promoter (harboring a putative CRE) was amplified by qPCR, and the proportion of immunoprecipitated DNA was calculated as relative to the non-immunoprecipitated input DNA in each sample. Subsequently, the ratio of relative expression was calculated between each SLE patient and the corresponding control individual. Horizontal lines represent mean values. B, the dotted line represents the H3K27 methylation status in control T cells, for each of which was set to 1.0. Changes in the methylation status in the matched SLE patient are given in the bar diagram (mean ± S.D.). C, total T cells from 15 healthy controls (light gray bar), 6 inactive SLE patients (dark gray bar), and 12 active SLE patients (black bar) were subjected <t>to</t> <t>methylated</t> DNA immunoprecipitation. The percentage of methylated DNA is given as mean ± S.D.
Lectin Swga, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/lectin swga/product/Vector Laboratories
Average 94 stars, based on 1 article reviews
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Image Search Results


Histone methylation and CpG DNA methylation of the Notch-1 promoter are increased in T cells from SLE patients. A, histone H3K27 methylation was analyzed in total T cells from three age-, gender-, and ethnicity-matched control (CON)/SLE pairs by ChIP assays. Dotted lines associate matched samples. A region of interest within the human Notch-1 promoter (harboring a putative CRE) was amplified by qPCR, and the proportion of immunoprecipitated DNA was calculated as relative to the non-immunoprecipitated input DNA in each sample. Subsequently, the ratio of relative expression was calculated between each SLE patient and the corresponding control individual. Horizontal lines represent mean values. B, the dotted line represents the H3K27 methylation status in control T cells, for each of which was set to 1.0. Changes in the methylation status in the matched SLE patient are given in the bar diagram (mean ± S.D.). C, total T cells from 15 healthy controls (light gray bar), 6 inactive SLE patients (dark gray bar), and 12 active SLE patients (black bar) were subjected to methylated DNA immunoprecipitation. The percentage of methylated DNA is given as mean ± S.D.

Journal: The Journal of Biological Chemistry

Article Title: cAMP-responsive Element Modulator ? (CREM?) Contributes to Decreased Notch-1 Expression in T Cells from Patients with Active Systemic Lupus Erythematosus (SLE) *

doi: 10.1074/jbc.M112.425371

Figure Lengend Snippet: Histone methylation and CpG DNA methylation of the Notch-1 promoter are increased in T cells from SLE patients. A, histone H3K27 methylation was analyzed in total T cells from three age-, gender-, and ethnicity-matched control (CON)/SLE pairs by ChIP assays. Dotted lines associate matched samples. A region of interest within the human Notch-1 promoter (harboring a putative CRE) was amplified by qPCR, and the proportion of immunoprecipitated DNA was calculated as relative to the non-immunoprecipitated input DNA in each sample. Subsequently, the ratio of relative expression was calculated between each SLE patient and the corresponding control individual. Horizontal lines represent mean values. B, the dotted line represents the H3K27 methylation status in control T cells, for each of which was set to 1.0. Changes in the methylation status in the matched SLE patient are given in the bar diagram (mean ± S.D.). C, total T cells from 15 healthy controls (light gray bar), 6 inactive SLE patients (dark gray bar), and 12 active SLE patients (black bar) were subjected to methylated DNA immunoprecipitation. The percentage of methylated DNA is given as mean ± S.D.

Article Snippet: Equal amounts (100 ng) of completely (100%) methylated human DNA and demethylated human DNA (Zymo Research) were included as the input and negative control.

Techniques: Methylation, DNA Methylation Assay, Amplification, Immunoprecipitation, Expressing