Journal:

Article Title: Web-Based Phylogenetic Assignment Tool for Analysis of Terminal Restriction Fragment Length Polymorphism Profiles of Microbial Communities

doi: 10.1128/AEM.69.11.6768-6776.2003

Figure Lengend Snippet: PAT output for samples collected from Devil's Lake (northern Wisconsin) in August 2002 a

Article Snippet: OPS 1 209.15 98.14 448.84 Clone SJA-121 211.83 164.89 455.96 Clone SJA-176 211.83 302.73 455.96 Clone Sva0115 211.83 497.52 797.46 Uncultured Holophaga sp. clone GuBH2-AD-9 211.83 186.71 448.84 Haemophilus influenzae-murium NCTC 11146 356.64 491.12 646.56 Oxobacter pfennigii DSM 3222 356.64 517.46 448.84 Strain AS2987 356.64 141.07 452.3 Strain AS2988 356.64 138.97 448.84 Haemophilus influenzae-murium NCTC 11146 358.24 488.77 646.56 Clostridium malenominatum ATCC 25776 359.96 517.46 448.84 Clostridium tetanomorphum DSM 528 359.96 517.46 448.84 Clostridium tetanomorphum strain H1 NCIMB 11547 359.96 517.46 448.84 Achromatium HK9 363.92 455.23 646.56/PICK> Actinobacillus salpingitidis CCUG 23139 363.92 497.52 646.56 Actinomycetales 363.92 159.63 455.96 Eubacterium lentum JCM 9979 363.92 133.14 452.3 Eubacterium lentum JCM 9979 363.92 133.14 455.96 Haemophilus haemoglobinophilus NCTC 1659 363.92 491.12 646.56 Haemophilus haemolyticus NCTC 10659 363.92 497.52 646.56 Haemophilus influenzae ATCC 33391 363.92 497.52 646.56 Haemophilus parainfluenzae ATCC 33392 363.92 497.52 743.54 Mycobacterium chubuense ATCC 27278 363.92 72.47 452.3 Oceanospirillum kriegii ATCC 27133 363.92 488.77 646.56 Pasteurella sp. strain CCUG 19794 363.92 497.52 646.56 Pasteurella sp. strain Bisgaard Taxon 15 CCUG 16500 363.92 497.52 646.56 Streptomyces bikiniensis DSM 40581 363.92 159.63 452.3 Marinomonas communis ATCC 27118 372.36 497.52 646.56 Oceanospirillum linum ATCC 11336 372.36 128.58 646.56 Uncultured bacterium; ASL8 372.36 72.47 96.23 Vibrio diazotrophicus strain NS ATCC 33466 372.36 497.52 646.56 Vibrio vulnificus ATCC 27562 372.36 497.52 646.56 Open in a separate window a The phylogenetic assignments are sorted by Hha I fragment size.

Techniques: Northern Blot

Journal:

Article Title: Rapid Detection of Clostridium difficile in Feces by Real-Time PCR

doi: 10.1128/JCM.41.2.730-734.2003

Figure Lengend Snippet: Bacterial strains used in this study

Article Snippet: The specificity of the PCR-based assay was demonstrated by using 14 non- C. difficile strains, which comprise 13 Clostridium species (Table ). table ft1 table-wrap mode="anchored" t5 TABLE 1. caption a7 Species (strain no.) Toxinotype Tcd production PCR detection Clostridium difficile (ATCC 9689) 0 TcdA and TcdB tcdA and tcdB Clostridium difficile (VPI 10463) a 0 TcdA and TcdB tcdA and tcdB Clostridium difficile (MD 32275) a 0 TcdA and TcdB tcdA and tcdB Clostridium difficile (MD 56484) a 0 TcdA and TcdB tcdA and tcdB Clostridium difficile (MD EX 623) a I TcdA and TcdB tcdA and tcdB Clostridium difficile (MD SE 847) a III TcdA and TcdB tcdB Clostridium difficile (MD 55767) a IV TcdA and TcdB tcdB Clostridium difficile (MD 51377) a VI TcdA and TcdB tcdB Clostridium difficile (MD 20376) a VIII TcdB tcdA and tcdB Clostridium beijerinckii (ATCC 8260) NA b − c − Clostridium bifermentans (ATCC 638) NA − − Clostridium botulinum (CHUL 20:1.2) NA − − Clostridium histolyticum (ATCC 19401) NA − − Clostridium innocuum (ATCC 14501) NA − − Clostridium novyi (ATCC 19402) NA − − Clostridium perfringens (ATCC 13124) NA − − Clostridium ramosum (ATCC 25582) NA − − Clostridium septicum (ATCC 12464) NA − − Clostridium sordellii (ATCC 9714) NA − − Clostridium sphenoides (ATCC 19403) NA − − Clostridium tertium (ATCC 14573) NA − − Clostridium tetani (ATCC 19406) NA − − Escherichia coli (ATCC 11775) NA − − Open in a separate window a C. difficile strains provided by Michel Delmée (Université Catholique de Louvain). b NA, not applicable. c −, negative.

Techniques: