Journal: Cancer cell

Article Title: A Menin-MLL inhibitor induces specific chromatin changes and eradicates disease in models of MLL -rearranged leukemia

doi: 10.1016/j.ccell.2019.11.001

Figure Lengend Snippet: (A) Volcano-plot of RNA-seq data obtained from MOLM13 cells treated with VTP50469. Selected MLL-fusion target genes are labeled.

Article Snippet: To determine steady-state plasma levels, four groups (n=6) of female BALB/c mice (Charles River Laboratories) were fed with various {"type":"entrez-protein","attrs":{"text":"VTP50469","term_id":"1773223164","term_text":"VTP50469"}} VTP50469 spiked-in chows for 6 days.

Techniques: RNA Sequencing Assay, Labeling

Journal: Cancer cell

Article Title: A Menin-MLL inhibitor induces specific chromatin changes and eradicates disease in models of MLL -rearranged leukemia

doi: 10.1016/j.ccell.2019.11.001

Figure Lengend Snippet: (A-B) Menin, MLL1n, DOT1L, and H3K79me2 ChIP-seq data in MOLM13 cells treated with VTP50469 or DMSO were plotted as (A) heat-map/tornado plots of TSS±3kb, (B) average/meta-plots of 113 MLL-AF9 targets TSS±3kb.

Article Snippet: To determine steady-state plasma levels, four groups (n=6) of female BALB/c mice (Charles River Laboratories) were fed with various {"type":"entrez-protein","attrs":{"text":"VTP50469","term_id":"1773223164","term_text":"VTP50469"}} VTP50469 spiked-in chows for 6 days.

Techniques: ChIP-sequencing

Journal: Cancer cell

Article Title: A Menin-MLL inhibitor induces specific chromatin changes and eradicates disease in models of MLL -rearranged leukemia

doi: 10.1016/j.ccell.2019.11.001

Figure Lengend Snippet: (A) Kaplan-Meyer survival curves of NSG mice engrafted with MV4;11 cells and dosed with VTP50469 as indicated. Green bar indicates the 28-day treatment period (n = 10).

Article Snippet: To determine steady-state plasma levels, four groups (n=6) of female BALB/c mice (Charles River Laboratories) were fed with various {"type":"entrez-protein","attrs":{"text":"VTP50469","term_id":"1773223164","term_text":"VTP50469"}} VTP50469 spiked-in chows for 6 days.

Techniques:

Journal: Cancer cell

Article Title: A Menin-MLL inhibitor induces specific chromatin changes and eradicates disease in models of MLL -rearranged leukemia

doi: 10.1016/j.ccell.2019.11.001

Figure Lengend Snippet: (A) %hCD45+ over time in the PB of NSG mice transplanted with an MLL-r B-ALL PDX (MLL-2) and treated with the indicated doses of VTP50469, BID. Treatment was administered for the first 28 days.

Article Snippet: To determine steady-state plasma levels, four groups (n=6) of female BALB/c mice (Charles River Laboratories) were fed with various {"type":"entrez-protein","attrs":{"text":"VTP50469","term_id":"1773223164","term_text":"VTP50469"}} VTP50469 spiked-in chows for 6 days.

Techniques:

Journal: Cancer cell

Article Title: A Menin-MLL inhibitor induces specific chromatin changes and eradicates disease in models of MLL -rearranged leukemia

doi: 10.1016/j.ccell.2019.11.001

Figure Lengend Snippet: (A) Heatmap representation of gene expression of 145 MLL-AF4 target genes expressed in MLL-r B-ALL PDX cells isolated from mice after 28 day dosing with control or VTP50469 chow.

Article Snippet: To determine steady-state plasma levels, four groups (n=6) of female BALB/c mice (Charles River Laboratories) were fed with various {"type":"entrez-protein","attrs":{"text":"VTP50469","term_id":"1773223164","term_text":"VTP50469"}} VTP50469 spiked-in chows for 6 days.

Techniques: Expressing, Isolation

Journal: Cancer cell

Article Title: A Menin-MLL inhibitor induces specific chromatin changes and eradicates disease in models of MLL -rearranged leukemia

doi: 10.1016/j.ccell.2019.11.001

Figure Lengend Snippet: (A) Molecules 1 through 4 in medicinal chemistry development of VTP50469. Dotted lines indicate VTP50469 potential interactions with Y276 and W341 of Menin.

Article Snippet: To determine steady-state plasma levels, four groups (n=6) of female BALB/c mice (Charles River Laboratories) were fed with various {"type":"entrez-protein","attrs":{"text":"VTP50469","term_id":"1773223164","term_text":"VTP50469"}} VTP50469 spiked-in chows for 6 days.

Techniques:

Journal: Cancer cell

Article Title: A Menin-MLL inhibitor induces specific chromatin changes and eradicates disease in models of MLL -rearranged leukemia

doi: 10.1016/j.ccell.2019.11.001

Figure Lengend Snippet: (A) H&E staining of the BM of naïve and MLL-2-engrafted mice treated with control or VTP50469 at Day 0, 28, and 328. Boxes indicate the %hCD45+. Bar, 10 μm.

Article Snippet: To determine steady-state plasma levels, four groups (n=6) of female BALB/c mice (Charles River Laboratories) were fed with various {"type":"entrez-protein","attrs":{"text":"VTP50469","term_id":"1773223164","term_text":"VTP50469"}} VTP50469 spiked-in chows for 6 days.

Techniques: Staining

Journal: Cancer cell

Article Title: A Menin-MLL inhibitor induces specific chromatin changes and eradicates disease in models of MLL -rearranged leukemia

doi: 10.1016/j.ccell.2019.11.001

Figure Lengend Snippet: Key Resources Table

Article Snippet: To determine steady-state plasma levels, four groups (n=6) of female BALB/c mice (Charles River Laboratories) were fed with various {"type":"entrez-protein","attrs":{"text":"VTP50469","term_id":"1773223164","term_text":"VTP50469"}} VTP50469 spiked-in chows for 6 days.

Techniques: Magnetic Beads, Recombinant, Sample Prep, Cell Isolation, Software