Journal: Acta Neuropathologica Communications

Article Title: MFG-E8 (LACTADHERIN): a novel marker associated with cerebral amyloid angiopathy

doi: 10.1186/s40478-021-01257-9

Figure Lengend Snippet: MFG-E8 expression in HBVSMCs after treatment with Aβ40 peptides. a Representative immunocytochemistry image showing the expression of MFG-E8 (green) and SMA (red) in HBVSMCs. b Cell viability after 2 and 5 days of treatment with 25 μM Aβ40 peptide and the toxic Aβ40-D peptide, n = 3–4/group. c Representative western blot images and quantification of MFG-E8 protein levels after 2 and 5 days of treatment with 25 μM Aβ40 peptide and the toxic Aβ40-D peptide, n = 3–4/group. d Cell viability of HBVSMCs co-treated with 2 µg/ml rhMFG-E8 and 25 μM Aβ40-D for 5 days (n = 5/group). e Cell viability of HBVSMCs treated with 25 μM Aβ40-D for 48 h after transfection with MFG-E8- or NT-siRNA (n = 5/group). A non-targeting siRNA (siNT) was used as a control. Significant differences among groups are indicated as * p < 0.05, ** p < 0.01, **** p < 0.0001

Article Snippet: Primary cultures of human brain vascular smooth muscle cells (HBVSMCs) were obtained from Innoprot (Derio, Bizkaia, Spain).

Techniques: Expressing, Immunocytochemistry, Western Blot, Transfection

Journal: Journal of Cardiovascular Translational Research

Article Title: Hif-1α/Slit2 Mediates Vascular Smooth Muscle Cell Phenotypic Changes in Restenosis of Bypass Grafts

doi: 10.1007/s12265-023-10384-8

Figure Lengend Snippet: Slit2 overexpression regulated the migration and proliferation of VSMCs. AAV-blank and AAV-Slit2( +) were transfected into VSMCs, and migration was detected by Transwell and wound-healing assays. Proliferation was measured by CCK8 and BrdU assays. A Western blotting was used to detect the efficiency of Slit2 overexpression with AAV-Slit2 ( +) transfected into VSMCs (β-actin was used as an internal control, n = 3, *** P < 0.001 with Student’s t test). B – C The effect of Slit2 overexpression on VSMC migration by Transwell assays ( n = 5, *** P < 0.001 with Student’s t test). D – E Wound healing was used to test the migration velocity of VSMCs ( n = 5, *** P < 0.001 with Student’s t test). F – G The proliferation of VSMCs was detected by CCK8 or BrdU assays ( n = 5, *** P < 0.001, **** P < 0.0001 with Student’s t test)

Article Snippet: Thus, we used AAV infected with VSMCs to overexpress Slit2 (the overexpression efficiency is shown in Fig. A) to determine whether Slit2 regulated the migration (tested by Transwell and wound-healing assays) and proliferation (tested by CCK8 and BrdU assays) of VSMCs.

Techniques: Over Expression, Migration, Transfection, Western Blot, Control

Journal: Journal of Cardiovascular Translational Research

Article Title: Hif-1α/Slit2 Mediates Vascular Smooth Muscle Cell Phenotypic Changes in Restenosis of Bypass Grafts

doi: 10.1007/s12265-023-10384-8

Figure Lengend Snippet: Slit2 knockdown regulated the migration and proliferation of VSMCs. Negative control (NC) and Slit2 siRNA were transfected into VSMCs, and migration was detected by Transwell and wound-healing assays. Proliferation was measured by CCK8 and BrdU assays. A Western blotting was used to detect the efficiency of Slit2 knockdown with Slit2 siRNA transfected into VSMCs (β-actin was used as an internal control, n = 3, ** P < 0.01 with Student’s t test). B – C The effect of Slit2 knockdown on VSMC migration by Transwell assays ( n = 5, **** P < 0.0001 with Student’s t test). D – E Wound healing was used to test the migration velocity of VSMCs ( n = 5, **** P < 0.0001 with Student’s t test). F – G The proliferation of VSMCs was detected by CCK8 or BrdU assays ( n = 5, *** P < 0.001, **** P < 0.0001 with Student’s t test)

Article Snippet: Thus, we used AAV infected with VSMCs to overexpress Slit2 (the overexpression efficiency is shown in Fig. A) to determine whether Slit2 regulated the migration (tested by Transwell and wound-healing assays) and proliferation (tested by CCK8 and BrdU assays) of VSMCs.

Techniques: Knockdown, Migration, Negative Control, Transfection, Western Blot, Control

Journal: Journal of Cardiovascular Translational Research

Article Title: Hif-1α/Slit2 Mediates Vascular Smooth Muscle Cell Phenotypic Changes in Restenosis of Bypass Grafts

doi: 10.1007/s12265-023-10384-8

Figure Lengend Snippet: Hypoxia downregulated Slit2 expression via Hif-1α. A DAPI (blue), Slit2 (green), Hif-1α (red), and αSMA (pink) were labeled on the arteries of the VGR and non-VGR groups. B The fluorescence intensity of Slit2 and Hif-1α ( n = 5, *** P < 0.001, **** P < 0.0001 with Student’s t test). C The ratio of colocalization of Slit2 and Hif-1α in α-SMA ( n = 5, **** P < 0.0001 with Student’s t test). D and E VSMCs were incubated with hypoxia for 1, 3, 6, and 9 h, and the expression of Slit2 and Hif-1α was detected by western blotting. β-actin was used as an internal control ( n = 5, ** P < 0.01 with Dunnett’s multiple comparison test). F VSMCs were treated with agonist or inhibitor under normoxia or hypoxia, and the expression was tested by western blotting (β-actin was used as an internal control, n = 5, **** P < 0.0001 with one-way ANOVA)

Article Snippet: Thus, we used AAV infected with VSMCs to overexpress Slit2 (the overexpression efficiency is shown in Fig. A) to determine whether Slit2 regulated the migration (tested by Transwell and wound-healing assays) and proliferation (tested by CCK8 and BrdU assays) of VSMCs.

Techniques: Expressing, Labeling, Fluorescence, Incubation, Western Blot, Control, Comparison

Journal: Journal of Cardiovascular Translational Research

Article Title: Hif-1α/Slit2 Mediates Vascular Smooth Muscle Cell Phenotypic Changes in Restenosis of Bypass Grafts

doi: 10.1007/s12265-023-10384-8

Figure Lengend Snippet: Overexpression of Slit2 delayed VGR and phenotypic switching of VSMCs in rats. AAV-blank and AAV-Slit2( +) were injected into the rats with abdominal aortic transplantation. The patency of aorta bypass grafts was observed by echocardiography, and phenotype switching of VSMCs was detected by western blotting. A Flowchart of the animal experiments. B and C The vascular diameter and PW velocity were measured ( n = 10, * P < 0.05, ** P < 0.01 with Student’s t test). D The number of different stenosis rates in the rats with abdominal aortic transplantation. E The Slit2, Hif-1α, contractile phenotype (αSMA and MYH11), and synthetic phenotype (OPN and MGP) were tested by western blots (β-actin was used as an internal control, n = 10, *** P < 0.001, **** P < 0.0001 with Student’s t test)

Article Snippet: Thus, we used AAV infected with VSMCs to overexpress Slit2 (the overexpression efficiency is shown in Fig. A) to determine whether Slit2 regulated the migration (tested by Transwell and wound-healing assays) and proliferation (tested by CCK8 and BrdU assays) of VSMCs.

Techniques: Over Expression, Injection, Transplantation Assay, Western Blot, Control