Journal: bioRxiv

Article Title: A Novel Hollow Fiber Infection Model (HFIM) for Antiviral PK/PD studies of CMV infection

doi: 10.64898/2026.03.13.710048

Figure Lengend Snippet: Circle and square lines represent the Towne (lab-adapted) and the Merlin (wild-type) strain, respectively. ( A, B ) represent the genome copies (copies/mL) and virus titer (PFU/mL), over time. ( C, D ) represent the log 10 fold change relative to quantified copies/mL and PFU/mL at day of inoculation.

Article Snippet: Human cytomegalovirus (HCMV) Towne (ATCC ® VR-977 TM) and the Merlin strains were used for the infection model.

Techniques: Virus

Journal: bioRxiv

Article Title: A Novel Hollow Fiber Infection Model (HFIM) for Antiviral PK/PD studies of CMV infection

doi: 10.64898/2026.03.13.710048

Figure Lengend Snippet: Ganciclovir treatment was administered 2 mg/hour every 12 hours, 3 days post-inoculation. Circle and square lines represent the control (no drug treatment) and drug treatment group. (A-D) represent the log10-fold change of genome copies (copies/mL) and virus titers (PFU/mL) for Towne and Merlin, respectively, over 72 hours post-treatment initiation.

Article Snippet: Human cytomegalovirus (HCMV) Towne (ATCC ® VR-977 TM) and the Merlin strains were used for the infection model.

Techniques: Control, Virus

Journal: GMS Hygiene and Infection Control

Article Title: Virucidal efficacy of a UV-C disinfection system for semi-critical medical devices according to EN 14476

doi: 10.3205/dgkh000622

Figure Lengend Snippet: lg TCID 50 /mL titers of Adenovirus Type 5 on glass test surface at initial inoculation, post-drying, and after UV-C treatment

Article Snippet: Adenovirus Type 5 (ATCC VR-5) had an initial titer of 7.83±0.40 lg TCID 50 /mL.

Techniques:

Journal: GMS Hygiene and Infection Control

Article Title: Virucidal efficacy of a UV-C disinfection system for semi-critical medical devices according to EN 14476

doi: 10.3205/dgkh000622

Figure Lengend Snippet: lg TCID 50 /mL titers of Adenovirus Type 5 on TPE-test surface at initial inoculation, post-drying, and after UV-C treatment

Article Snippet: Adenovirus Type 5 (ATCC VR-5) had an initial titer of 7.83±0.40 lg TCID 50 /mL.

Techniques:

Journal: bioRxiv

Article Title: IFI207 promotes antiviral responses by modulating STING ubiquitination and degradation

doi: 10.64898/2026.03.05.709838

Figure Lengend Snippet: IFI207 promotes STING signaling in response to virus infection. A) and B) BMDMs (A) and BMDCs (B) from the indicated mice were infected HSV-1 for 6 hr (MOI=5), and western blots examining STING and phospho-STING levels were measured. Shown to the right is the quantification of three independent experiments. Two-way ANOVA was used to determine significance. ***, P ≤0.0008; ****, P ≤0.0001 B) IFNβ mRNA levels following HSV-1 infection were quantified by RT-qPCR. Shown is the average of 3 experiments. Two-way ANOVA was used to determine significance. ****, P ≤0.0001. (C) HSV DNA levels in the olfactory bulbs of wild type or IFI207 KO mice 6 days after intranasal inoculation of HSV McKrae. were quantified by qPCR. Welch’s t test was used to determine significance. **, P ≤0.004. D) Pups of the indicated genotype were infected with 25,000 PFU of MLV. At 16 dpi, splenic viral titers were determined on NIH3T3 cells by infectious center (IC) assays. Each dot represents an individual mouse. Significance was determined by one-way ANOVA. *, P ≤0.05; **, P ≤0.001; ***, P ≤0.00003; ***, P ≤0.0001

Article Snippet: HSV-1 (KOS strain; ATCC VR1493) was used for in vitro experiments.

Techniques: Virus, Infection, Western Blot, Quantitative RT-PCR, Olfactory

Journal: bioRxiv

Article Title: IFI207 promotes antiviral responses by modulating STING ubiquitination and degradation

doi: 10.64898/2026.03.05.709838

Figure Lengend Snippet: IFI207 promotes STING signaling in response to virus infection. A) BMDMs and fibroblasts from the indicated mice were infected HSV-1 for 6 hr (MOI=5), IFNβ mRNA levels were quantified by RT-qPCR. Shown is the average of 3 experiments. Two-way ANOVA was used to determine significance. ****, P≤0.0001. B) average weight loss in IFI207 KO and wild type mice at 5- and 6-days post-infection.

Article Snippet: HSV-1 (KOS strain; ATCC VR1493) was used for in vitro experiments.

Techniques: Virus, Infection, Quantitative RT-PCR

Journal: bioRxiv

Article Title: Alignment-Free Guided Design of a Pan-Orthoflavivirus RT-qPCR Assay

doi: 10.64898/2026.03.17.712358

Figure Lengend Snippet: (A) Faceted bar charts show Ct values measured across input copy numbers (copies per µL) for each virus—dengue virus (DENV1–4), Japanese encephalitis virus (JEV), West Nile virus (WNV), yellow fever virus (YFV), and Zika virus (ZIKV). Bars summarize Ct at each concentration, with points indicating individual reactions (technical replicates); red diamonds denote the mean (error bars, where shown, indicate variability across replicates). Across DENV1–4, ZIKV, and JEV (triplicates), Ct values generally decrease as input concentration increases. ZIKV shows greater replicate-to-replicate variation at some concentrations, reflected by wider error bars compared to other targets. WNV and YFV were detected at 1,000 copies per µL; only a single concentration was tested for these viruses due to limited sample material. Together, these results indicate broad target coverage and reliable amplification across a wide input range for the main panel members. (B) Limit of detection (LOD) assessment of the designed primer-probe set. Prior to testing clinical specimens, LOD was evaluated using nucleic acids from 6 arboviruses (DENV1–4, ZIKV, and JEV) at 1, 10, 1000 copies per µL, with 12 technical replicates per dilution. The assay detected DENV3, DENV4, and JEV down to 1 copy per µL, whereas DENV1, DENV2, and ZIKV were consistently detected down to 10 copies per µL. Points represent individual reaction; a diamond indicates the mean Ct, and error bars indicate ±SD. ND indicates not detected (did not meet the ≥95% positivity criterion and therefore did not qualify as the LOD). (C) Paired comparison of Ct values between assays in clinical samples. Ct values obtained with the commercial kit (Com; circles) and the designed primer–probe set (Des; triangles) are shown for each target, with paired measurements from the same sample connected by dashed lines, for 100 positive samples and 50 positive samples (20 positive with Chikungunya virus [CHIKV] and 30 others). Box plots summarize the distributions (center line, median; box, interquartile range; whiskers, range). Relative to the commercial kit, the designed primer–probe set detected DENV1–4 significantly earlier (lower Ct; p = 0.0004 [n = 25], 0.0013 [n=25], <0.0001 [n=11] and <0.0001 [n=25] for DENV1–4, respectively) but detected ZIKV later (higher Ct; p < 0.0001 [n =14]). P values were calculated using a two-sided paired t-test on matched Com–Des Ct values for each target. Overall diagnostic performance is reported in Tables 1,2 .

Article Snippet: Reference viruses were obtained from the American Type Culture Collection (ATCC), including DENV1 strain Hawaii (ATCC VR-1856), DENV2 strain TH-36 (ATCC VR-1810), DENV3 strain H87 (ATCC VR-3380), DENV4 H241 (ATCC VR-1490), and ZIKV strain PRVABC59 (ATCC VR-1843).

Techniques: Virus, Concentration Assay, Amplification, Comparison, Diagnostic Assay