Journal: Molecular Biotechnology

Article Title: Development of a Quantitative Real-Time Nucleic Acid Sequence-Based Amplification Assay with an Internal Control Using Molecular Beacon Probes for Selective and Sensitive Detection of Human Rhinovirus Serotypes

doi: 10.1007/s12033-011-9432-4

Figure Lengend Snippet: Evaluation of real-time NASBA specificity with HRV isolates and respiratory viruses other than HRV

Article Snippet: Human parainfluenza virus 1 , ATCC VR-94 , −.

Techniques: Virus

Journal: Scanning

Article Title: Ultrastructure changes induced by dry film formation of a trisodium phosphate blend, antimicrobial solution.

doi: 10.1002/sca.4950250602

Figure Lengend Snippet: FIG. 5 Scanning electron microscopy images of feline calicivirus ATCC VR-782. (a) Untreated feline calicivirus in normal MEM (field width 200 nm, magnification × 450,000). (b) KleenAll-treated feline calicivirus (field width 200 nm, magnification × 400,000). (c) KleenAll-treated feline calicivirus (field width 600 nm, magnifica- tion × 120,000).

Article Snippet: While the host cell line, feline kidney cell CRFK (ATCC CCL-94), showed a clear cytopathic effect (CPE, Fig. 6a) when infected with the untreated feline calicivirus (ATCC VR-782), there was no CPE on cells charged with the TSP blend-treated virus (Fig. 6b).

Techniques: Electron Microscopy

Journal: Scanning

Article Title: Ultrastructure changes induced by dry film formation of a trisodium phosphate blend, antimicrobial solution.

doi: 10.1002/sca.4950250602

Figure Lengend Snippet: FIG. 6 Light microscopy images of feline kidney cell line CRFK (ATCC CCL-94) infected by feline calicivirus ATCC VR-782. (a) In- fected by untreated feline calicivirus (field width 750 microns, mag- nification × 100). (b) Infected by KleenAll-treated feline calicivirus (field width 750 microns, magnification × 100).

Article Snippet: While the host cell line, feline kidney cell CRFK (ATCC CCL-94), showed a clear cytopathic effect (CPE, Fig. 6a) when infected with the untreated feline calicivirus (ATCC VR-782), there was no CPE on cells charged with the TSP blend-treated virus (Fig. 6b).

Techniques: Light Microscopy, Infection

Journal: European journal of medicinal chemistry

Article Title: Design, synthesis and evaluation of novel polypharmacological antichlamydial agents.

doi: 10.1016/j.ejmech.2015.07.019

Figure Lengend Snippet: Fig. 2. Inhibition of C. trachomatis growth by compounds 11c, 11d and the acylated sulfonamide 16 at 50 mM concentration in presence of 0.08e250 mM iron in the me- dium. Data processed with GrapPadPrism 6.0, transformed to logarithmic scale and curve fitted, mean and SD are shown (n ¼ 3).

Article Snippet: HeLa 229 cells (CCL-2.1, ATCC, Manassas, VA) were infected independently with C. trachomatis L2 serovar (VR-902B, ATCC) and C. pneumoniae strain T45 [26].

Techniques: Inhibition, Concentration Assay, Transformation Assay

Journal: Cell

Article Title: Therapeutic alphavirus cross-reactive E1 human antibodies inhibit viral egress.

doi: 10.1016/j.cell.2021.07.033

Figure Lengend Snippet: Figure 7. Model for mechanism of action of human E1-specific mAbs (A) Human E1-specific mAbs that target cryptic or partially exposed epitopes do not inhibit SINV/EEEV egress. Exposure for cryptic epitopes depends on pre- treatment conditions, such as acidic pH or addition of the nonionic detergent Tween 20. Treatment efficacy (EEEV-138 and EEEV-346) of EEEV infection following s.c. challenge is minimally significant due to low survival efficacy and presence of viral RNA levels in the serum of treated mice. (B) Human E1-specific mAbs that target exposed, pH-independent epitopes can inhibit SINV/EEEV egress. Quaternary epitopes between two adjacent E1 proteins of neighboring trimeric spikes may aid in binding to infected cells for inhibition of virus egress and enable broad alphavirus cross-reactivity (EEEV-179 [New World]). Treatment efficacy (EEEV-109 and -179) of EEEV infection following s.c. challenge corresponds with SINV/EEEV egress inhibition potency due to

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies EEEV-76 (hybridoma-produced IgG1) This paper N/A EEEV-104 (hybridoma-produced IgG1) This paper N/A EEEV-109 (hybridoma-produced IgG1) This paper N/A EEEV-126 (hybridoma-produced IgG1) This paper N/A EEEV-127 (hybridoma-produced IgG1) This paper N/A EEEV-138 (hybridoma-produced IgG1) This paper N/A EEEV-157 (hybridoma-produced IgG1) This paper N/A EEEV-179 (hybridoma-produced IgG1) This paper N/A EEEV-307 (hybridoma-produced IgG1) This paper N/A EEEV-312 (hybridoma-produced IgG1) This paper N/A EEEV-320 (hybridoma-produced IgG1) This paper N/A EEEV-342 (hybridoma-produced IgG1) This paper N/A EEEV-346 (hybridoma-produced IgG1) This paper N/A EEEV-354 (hybridoma-produced IgG1) This paper N/A EEEV-368 (hybridoma-produced IgG1) This paper N/A EEEV-377 (hybridoma-produced IgG1) This paper N/A EEEV-379 (hybridoma-produced IgG1) This paper N/A EEEV-387 (hybridoma-produced IgG1) This paper N/A EEEV-398 (hybridoma-produced IgG1) This paper N/A EEEV-400 (hybridoma-produced IgG1) This paper N/A rEEEV-97 IgG (recombinant Expi293F-produced IgG1) Williamson et al., 2020 N/A rEEEV-109 IgG (recombinant Expi293F-produced IgG1) This paper N/A rEEEV-109 LALA-PG (recombinant Expi293F-produced IgG1) This paper N/A rEEEV-126 IgG (recombinant Expi293F-produced IgG1) This paper N/A rEEEV-157 IgG (recombinant Expi293F-produced IgG1) This paper N/A rEEEV-346 IgG (recombinant Expi293F-produced IgG1) This paper N/A rDENV-2D22 IgG (recombinant ExpiCHO-produced IgG1) Fibriansah et al., 2015 N/A Murine mAb: EEEV-66 Michael S. Diamond Kim et al., 2019 Eastern equine encephalomyelitis immune ascites fluid ATCC Cat# VR-1242AF Venezuelan equine encephalitis virus hyperimmune ascitic fluid ATCC Cat# VR-1249AF Western equine encephalomyelitis virus immune ascitic fluid ATCC Cat# VR-1251AF Chikungunya virus immune ascitic fluid ATCC Cat# VR-1241AF Mayaro virus immune ascitic fluid ATCC Cat# VR-1277AF 1A4B-6 Millipore Cat# MAB8754; RRID:AB_11211668 1A3B-7 Millipore Cat# MAB8755; RRID:AB_95409 2A3D-5 Millipore Cat#MAB8746; RRID:AB_95399 (Continued on next page) Cell 184, 4430–4446.e1–e12, August 19, 2021 e1

Techniques: Infection, Binding Assay, Inhibition, Virus

Journal: Cells

Article Title: Mitochondrial ROS–ER Stress Axis Governs IL-10 Production in Neutrophils and Regulates Inflammation in Murine Chlamydia pneumoniae Lung Infection

doi: 10.3390/cells14191523

Figure Lengend Snippet: Live C. pneumoniae infection in mouse neutrophils elicits strong IL-10 production via TLR2. ( A ) Neutrophils were infected with C. pneumoniae (strain AR-39) at MOI = 1. mRNA was isolated from neutrophils after 24 h of infection. Expression of the indicated gene was confirmed by real-time PCR. ( B ) IL-10 levels in the supernatant were measured by ELISA. The supernatant from neutrophils infected with AR-39 at the specified MOI was collected after 24 h. ( C ) Neutrophils were infected with live or heat-killed C. pneumoniae strain AR-39 or strain CWL-029 at MOI = 1. The culture medium was collected 24 h post-infection. IL-10 in the medium was measured by ELISA. ( D ) Neutrophils from WT mice were infected with MOI = 1 AR-39 and cultured with TLR2 inhibitor C29 (100 μM), TLR4 inhibitor TAK-242 (1 μM), or TLR9 inhibitor E6446 (500 nM). Culture medium was collected 24 h after infection, and IL-10 levels were measured by ELISA. ( E ) Neutrophils from WT mice were infected with MOI = 1 AR-39 and co-cultured with or without TLR2 inhibitor C29 (100 μM) for 24 h, then stained with FITC-conjugated anti-chlamydia antibody. The bar length was 10 μm. ( F ) Neutrophils infected with MOI = 1 AR-39 were cultured with TLR2 inhibitor C29. After 24 h, total RNA was isolated, and C. pneumoniae 16S ribosomal RNA was quantified by real-time PCR. ( G ) Neutrophils from WT and TLR2 −/− mice were infected with MOI = 1 AR-39. Culture supernatants were collected after 24 h, and IL-10 levels were measured via ELISA. Statistical analyses were performed using an unpaired two-tailed Student’s t -test ( A , F ), a one-way ANOVA followed by Tukey’s multiple comparison test ( B , D ), and a two-way ANOVA followed by Tukey’s multiple comparison test ( C , G ). * p < 0.05, ** p < 0.01, and **** p < 0.0001, Each experiment was conducted at least three times with n ≥ 3, and the data presented are representative of these results. Cpn: C. pneumoniae .

Article Snippet: C. pneumoniae (strain: AR-39, ATCC 53592, and strain: CWL-029, ATCC VR-1310) and Chlamydia muridarum mouse pneumonitis (MoPn) (strain: Nigg II, ATCC VR-123) were purchased from the American Type Culture Collection.

Techniques: Infection, Isolation, Expressing, Real-time Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay, Cell Culture, Staining, Two Tailed Test, Comparison