voltage Search Results


hvcn1  (Alomone Labs)
92
Alomone Labs hvcn1
Hvcn1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hvcn1/product/Alomone Labs
Average 92 stars, based on 1 article reviews
hvcn1 - by Bioz Stars, 2026-05
92/100 stars
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electrometer  (Danaher Inc)
96
Danaher Inc electrometer
Electrometer, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/electrometer/product/Danaher Inc
Average 96 stars, based on 1 article reviews
electrometer - by Bioz Stars, 2026-05
96/100 stars
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ser503 phospho kv3 1β  (PhosphoSolutions)
96
PhosphoSolutions ser503 phospho kv3 1β
5-HT5A signaling in PV neurons. a Bar graph summary of cAMP level in N2A cells transiently transfected with mouse 5-HT5A. The cells were incubated with the indicated amounts of forskolin and 5-HT. One representative experiment out of three is shown. Bars represent means of cAMP level ( n = 4 wells per group) as percentage of baseline + SD. One Way ANOVA, * P < 0.05 vs. baseline. b Representative western blot images (top) and bar graph summary (bottom) depicting the levels of <t>Kv3.1β</t> and <t>Ser503</t> pKv3.1β in transfected HEK293 cells treated as indicated ( n = 4 wells per condition). Bars represent mean densities of the pKv normalized to that of the total Kv. Number and arrowhead indicate protein size in kDa. One-way ANOVA. * P < 0.05, ** P < 0.01. OA okadaic acid, PMA Phorbol 12-myristate 13-acetate. c Representative traces of Kv potassium currents evoked with 10 mV potential steps from −70 to +50 mV in PV DG neurons from Veh- and Flx-treated WT mice. d PMA (200 nM) decreased the amplitude of KV currents in Flx-treated mice ( n = 4, 3) but not Veh-treated WT mice ( n = 3, 3). RM one-way ANOVA, * P < 0.05. e Model for serotonergic signaling in PV hippocampal cells. Left: initial exposure to SSRIs does not alter cAMP levels and PKA activity. PP2A activity remains high and Kv3.1β is dephosphorylated. Right: chronic SSRIs increases 5-HT5A surface levels. 5-HT5AR activation reduces cAMP levels and diminishes PKA and PP2A activities to reduce Ser 503 Kv3.1β dephosphorylation
Ser503 Phospho Kv3 1β, supplied by PhosphoSolutions, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ser503 phospho kv3 1β/product/PhosphoSolutions
Average 96 stars, based on 1 article reviews
ser503 phospho kv3 1β - by Bioz Stars, 2026-05
96/100 stars
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two electrode voltage clamp  (ADInstruments)
85
ADInstruments two electrode voltage clamp
5-HT5A signaling in PV neurons. a Bar graph summary of cAMP level in N2A cells transiently transfected with mouse 5-HT5A. The cells were incubated with the indicated amounts of forskolin and 5-HT. One representative experiment out of three is shown. Bars represent means of cAMP level ( n = 4 wells per group) as percentage of baseline + SD. One Way ANOVA, * P < 0.05 vs. baseline. b Representative western blot images (top) and bar graph summary (bottom) depicting the levels of <t>Kv3.1β</t> and <t>Ser503</t> pKv3.1β in transfected HEK293 cells treated as indicated ( n = 4 wells per condition). Bars represent mean densities of the pKv normalized to that of the total Kv. Number and arrowhead indicate protein size in kDa. One-way ANOVA. * P < 0.05, ** P < 0.01. OA okadaic acid, PMA Phorbol 12-myristate 13-acetate. c Representative traces of Kv potassium currents evoked with 10 mV potential steps from −70 to +50 mV in PV DG neurons from Veh- and Flx-treated WT mice. d PMA (200 nM) decreased the amplitude of KV currents in Flx-treated mice ( n = 4, 3) but not Veh-treated WT mice ( n = 3, 3). RM one-way ANOVA, * P < 0.05. e Model for serotonergic signaling in PV hippocampal cells. Left: initial exposure to SSRIs does not alter cAMP levels and PKA activity. PP2A activity remains high and Kv3.1β is dephosphorylated. Right: chronic SSRIs increases 5-HT5A surface levels. 5-HT5AR activation reduces cAMP levels and diminishes PKA and PP2A activities to reduce Ser 503 Kv3.1β dephosphorylation
Two Electrode Voltage Clamp, supplied by ADInstruments, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 85 stars, based on 1 article reviews
two electrode voltage clamp - by Bioz Stars, 2026-05
85/100 stars
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vdac rabbit pab  (Rockland Immunochemicals)
91
Rockland Immunochemicals vdac rabbit pab
5-HT5A signaling in PV neurons. a Bar graph summary of cAMP level in N2A cells transiently transfected with mouse 5-HT5A. The cells were incubated with the indicated amounts of forskolin and 5-HT. One representative experiment out of three is shown. Bars represent means of cAMP level ( n = 4 wells per group) as percentage of baseline + SD. One Way ANOVA, * P < 0.05 vs. baseline. b Representative western blot images (top) and bar graph summary (bottom) depicting the levels of <t>Kv3.1β</t> and <t>Ser503</t> pKv3.1β in transfected HEK293 cells treated as indicated ( n = 4 wells per condition). Bars represent mean densities of the pKv normalized to that of the total Kv. Number and arrowhead indicate protein size in kDa. One-way ANOVA. * P < 0.05, ** P < 0.01. OA okadaic acid, PMA Phorbol 12-myristate 13-acetate. c Representative traces of Kv potassium currents evoked with 10 mV potential steps from −70 to +50 mV in PV DG neurons from Veh- and Flx-treated WT mice. d PMA (200 nM) decreased the amplitude of KV currents in Flx-treated mice ( n = 4, 3) but not Veh-treated WT mice ( n = 3, 3). RM one-way ANOVA, * P < 0.05. e Model for serotonergic signaling in PV hippocampal cells. Left: initial exposure to SSRIs does not alter cAMP levels and PKA activity. PP2A activity remains high and Kv3.1β is dephosphorylated. Right: chronic SSRIs increases 5-HT5A surface levels. 5-HT5AR activation reduces cAMP levels and diminishes PKA and PP2A activities to reduce Ser 503 Kv3.1β dephosphorylation
Vdac Rabbit Pab, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/vdac rabbit pab/product/Rockland Immunochemicals
Average 91 stars, based on 1 article reviews
vdac rabbit pab - by Bioz Stars, 2026-05
91/100 stars
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axoclamp 900a amplifier  (Danaher Inc)
96
Danaher Inc axoclamp 900a amplifier
5-HT5A signaling in PV neurons. a Bar graph summary of cAMP level in N2A cells transiently transfected with mouse 5-HT5A. The cells were incubated with the indicated amounts of forskolin and 5-HT. One representative experiment out of three is shown. Bars represent means of cAMP level ( n = 4 wells per group) as percentage of baseline + SD. One Way ANOVA, * P < 0.05 vs. baseline. b Representative western blot images (top) and bar graph summary (bottom) depicting the levels of <t>Kv3.1β</t> and <t>Ser503</t> pKv3.1β in transfected HEK293 cells treated as indicated ( n = 4 wells per condition). Bars represent mean densities of the pKv normalized to that of the total Kv. Number and arrowhead indicate protein size in kDa. One-way ANOVA. * P < 0.05, ** P < 0.01. OA okadaic acid, PMA Phorbol 12-myristate 13-acetate. c Representative traces of Kv potassium currents evoked with 10 mV potential steps from −70 to +50 mV in PV DG neurons from Veh- and Flx-treated WT mice. d PMA (200 nM) decreased the amplitude of KV currents in Flx-treated mice ( n = 4, 3) but not Veh-treated WT mice ( n = 3, 3). RM one-way ANOVA, * P < 0.05. e Model for serotonergic signaling in PV hippocampal cells. Left: initial exposure to SSRIs does not alter cAMP levels and PKA activity. PP2A activity remains high and Kv3.1β is dephosphorylated. Right: chronic SSRIs increases 5-HT5A surface levels. 5-HT5AR activation reduces cAMP levels and diminishes PKA and PP2A activities to reduce Ser 503 Kv3.1β dephosphorylation
Axoclamp 900a Amplifier, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/axoclamp 900a amplifier/product/Danaher Inc
Average 96 stars, based on 1 article reviews
axoclamp 900a amplifier - by Bioz Stars, 2026-05
96/100 stars
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kv1 5 antibody  (Alomone Labs)
93
Alomone Labs kv1 5 antibody
5-HT5A signaling in PV neurons. a Bar graph summary of cAMP level in N2A cells transiently transfected with mouse 5-HT5A. The cells were incubated with the indicated amounts of forskolin and 5-HT. One representative experiment out of three is shown. Bars represent means of cAMP level ( n = 4 wells per group) as percentage of baseline + SD. One Way ANOVA, * P < 0.05 vs. baseline. b Representative western blot images (top) and bar graph summary (bottom) depicting the levels of <t>Kv3.1β</t> and <t>Ser503</t> pKv3.1β in transfected HEK293 cells treated as indicated ( n = 4 wells per condition). Bars represent mean densities of the pKv normalized to that of the total Kv. Number and arrowhead indicate protein size in kDa. One-way ANOVA. * P < 0.05, ** P < 0.01. OA okadaic acid, PMA Phorbol 12-myristate 13-acetate. c Representative traces of Kv potassium currents evoked with 10 mV potential steps from −70 to +50 mV in PV DG neurons from Veh- and Flx-treated WT mice. d PMA (200 nM) decreased the amplitude of KV currents in Flx-treated mice ( n = 4, 3) but not Veh-treated WT mice ( n = 3, 3). RM one-way ANOVA, * P < 0.05. e Model for serotonergic signaling in PV hippocampal cells. Left: initial exposure to SSRIs does not alter cAMP levels and PKA activity. PP2A activity remains high and Kv3.1β is dephosphorylated. Right: chronic SSRIs increases 5-HT5A surface levels. 5-HT5AR activation reduces cAMP levels and diminishes PKA and PP2A activities to reduce Ser 503 Kv3.1β dephosphorylation
Kv1 5 Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/kv1 5 antibody/product/Alomone Labs
Average 93 stars, based on 1 article reviews
kv1 5 antibody - by Bioz Stars, 2026-05
93/100 stars
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anti cav3 2  (Alomone Labs)
93
Alomone Labs anti cav3 2
5-HT5A signaling in PV neurons. a Bar graph summary of cAMP level in N2A cells transiently transfected with mouse 5-HT5A. The cells were incubated with the indicated amounts of forskolin and 5-HT. One representative experiment out of three is shown. Bars represent means of cAMP level ( n = 4 wells per group) as percentage of baseline + SD. One Way ANOVA, * P < 0.05 vs. baseline. b Representative western blot images (top) and bar graph summary (bottom) depicting the levels of <t>Kv3.1β</t> and <t>Ser503</t> pKv3.1β in transfected HEK293 cells treated as indicated ( n = 4 wells per condition). Bars represent mean densities of the pKv normalized to that of the total Kv. Number and arrowhead indicate protein size in kDa. One-way ANOVA. * P < 0.05, ** P < 0.01. OA okadaic acid, PMA Phorbol 12-myristate 13-acetate. c Representative traces of Kv potassium currents evoked with 10 mV potential steps from −70 to +50 mV in PV DG neurons from Veh- and Flx-treated WT mice. d PMA (200 nM) decreased the amplitude of KV currents in Flx-treated mice ( n = 4, 3) but not Veh-treated WT mice ( n = 3, 3). RM one-way ANOVA, * P < 0.05. e Model for serotonergic signaling in PV hippocampal cells. Left: initial exposure to SSRIs does not alter cAMP levels and PKA activity. PP2A activity remains high and Kv3.1β is dephosphorylated. Right: chronic SSRIs increases 5-HT5A surface levels. 5-HT5AR activation reduces cAMP levels and diminishes PKA and PP2A activities to reduce Ser 503 Kv3.1β dephosphorylation
Anti Cav3 2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti cav3 2/product/Alomone Labs
Average 93 stars, based on 1 article reviews
anti cav3 2 - by Bioz Stars, 2026-05
93/100 stars
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kcnd2  (Alomone Labs)
93
Alomone Labs kcnd2
5-HT5A signaling in PV neurons. a Bar graph summary of cAMP level in N2A cells transiently transfected with mouse 5-HT5A. The cells were incubated with the indicated amounts of forskolin and 5-HT. One representative experiment out of three is shown. Bars represent means of cAMP level ( n = 4 wells per group) as percentage of baseline + SD. One Way ANOVA, * P < 0.05 vs. baseline. b Representative western blot images (top) and bar graph summary (bottom) depicting the levels of <t>Kv3.1β</t> and <t>Ser503</t> pKv3.1β in transfected HEK293 cells treated as indicated ( n = 4 wells per condition). Bars represent mean densities of the pKv normalized to that of the total Kv. Number and arrowhead indicate protein size in kDa. One-way ANOVA. * P < 0.05, ** P < 0.01. OA okadaic acid, PMA Phorbol 12-myristate 13-acetate. c Representative traces of Kv potassium currents evoked with 10 mV potential steps from −70 to +50 mV in PV DG neurons from Veh- and Flx-treated WT mice. d PMA (200 nM) decreased the amplitude of KV currents in Flx-treated mice ( n = 4, 3) but not Veh-treated WT mice ( n = 3, 3). RM one-way ANOVA, * P < 0.05. e Model for serotonergic signaling in PV hippocampal cells. Left: initial exposure to SSRIs does not alter cAMP levels and PKA activity. PP2A activity remains high and Kv3.1β is dephosphorylated. Right: chronic SSRIs increases 5-HT5A surface levels. 5-HT5AR activation reduces cAMP levels and diminishes PKA and PP2A activities to reduce Ser 503 Kv3.1β dephosphorylation
Kcnd2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/kcnd2/product/Alomone Labs
Average 93 stars, based on 1 article reviews
kcnd2 - by Bioz Stars, 2026-05
93/100 stars
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anti cav1 2 channel  (Alomone Labs)
96
Alomone Labs anti cav1 2 channel
5-HT5A signaling in PV neurons. a Bar graph summary of cAMP level in N2A cells transiently transfected with mouse 5-HT5A. The cells were incubated with the indicated amounts of forskolin and 5-HT. One representative experiment out of three is shown. Bars represent means of cAMP level ( n = 4 wells per group) as percentage of baseline + SD. One Way ANOVA, * P < 0.05 vs. baseline. b Representative western blot images (top) and bar graph summary (bottom) depicting the levels of <t>Kv3.1β</t> and <t>Ser503</t> pKv3.1β in transfected HEK293 cells treated as indicated ( n = 4 wells per condition). Bars represent mean densities of the pKv normalized to that of the total Kv. Number and arrowhead indicate protein size in kDa. One-way ANOVA. * P < 0.05, ** P < 0.01. OA okadaic acid, PMA Phorbol 12-myristate 13-acetate. c Representative traces of Kv potassium currents evoked with 10 mV potential steps from −70 to +50 mV in PV DG neurons from Veh- and Flx-treated WT mice. d PMA (200 nM) decreased the amplitude of KV currents in Flx-treated mice ( n = 4, 3) but not Veh-treated WT mice ( n = 3, 3). RM one-way ANOVA, * P < 0.05. e Model for serotonergic signaling in PV hippocampal cells. Left: initial exposure to SSRIs does not alter cAMP levels and PKA activity. PP2A activity remains high and Kv3.1β is dephosphorylated. Right: chronic SSRIs increases 5-HT5A surface levels. 5-HT5AR activation reduces cAMP levels and diminishes PKA and PP2A activities to reduce Ser 503 Kv3.1β dephosphorylation
Anti Cav1 2 Channel, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti cav1 2 channel/product/Alomone Labs
Average 96 stars, based on 1 article reviews
anti cav1 2 channel - by Bioz Stars, 2026-05
96/100 stars
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l type ca channel  (Alomone Labs)
91
Alomone Labs l type ca channel
5-HT5A signaling in PV neurons. a Bar graph summary of cAMP level in N2A cells transiently transfected with mouse 5-HT5A. The cells were incubated with the indicated amounts of forskolin and 5-HT. One representative experiment out of three is shown. Bars represent means of cAMP level ( n = 4 wells per group) as percentage of baseline + SD. One Way ANOVA, * P < 0.05 vs. baseline. b Representative western blot images (top) and bar graph summary (bottom) depicting the levels of <t>Kv3.1β</t> and <t>Ser503</t> pKv3.1β in transfected HEK293 cells treated as indicated ( n = 4 wells per condition). Bars represent mean densities of the pKv normalized to that of the total Kv. Number and arrowhead indicate protein size in kDa. One-way ANOVA. * P < 0.05, ** P < 0.01. OA okadaic acid, PMA Phorbol 12-myristate 13-acetate. c Representative traces of Kv potassium currents evoked with 10 mV potential steps from −70 to +50 mV in PV DG neurons from Veh- and Flx-treated WT mice. d PMA (200 nM) decreased the amplitude of KV currents in Flx-treated mice ( n = 4, 3) but not Veh-treated WT mice ( n = 3, 3). RM one-way ANOVA, * P < 0.05. e Model for serotonergic signaling in PV hippocampal cells. Left: initial exposure to SSRIs does not alter cAMP levels and PKA activity. PP2A activity remains high and Kv3.1β is dephosphorylated. Right: chronic SSRIs increases 5-HT5A surface levels. 5-HT5AR activation reduces cAMP levels and diminishes PKA and PP2A activities to reduce Ser 503 Kv3.1β dephosphorylation
L Type Ca Channel, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/l type ca channel/product/Alomone Labs
Average 91 stars, based on 1 article reviews
l type ca channel - by Bioz Stars, 2026-05
91/100 stars
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protein product number vendor  (Rockland Immunochemicals)
90
Rockland Immunochemicals protein product number vendor
5-HT5A signaling in PV neurons. a Bar graph summary of cAMP level in N2A cells transiently transfected with mouse 5-HT5A. The cells were incubated with the indicated amounts of forskolin and 5-HT. One representative experiment out of three is shown. Bars represent means of cAMP level ( n = 4 wells per group) as percentage of baseline + SD. One Way ANOVA, * P < 0.05 vs. baseline. b Representative western blot images (top) and bar graph summary (bottom) depicting the levels of <t>Kv3.1β</t> and <t>Ser503</t> pKv3.1β in transfected HEK293 cells treated as indicated ( n = 4 wells per condition). Bars represent mean densities of the pKv normalized to that of the total Kv. Number and arrowhead indicate protein size in kDa. One-way ANOVA. * P < 0.05, ** P < 0.01. OA okadaic acid, PMA Phorbol 12-myristate 13-acetate. c Representative traces of Kv potassium currents evoked with 10 mV potential steps from −70 to +50 mV in PV DG neurons from Veh- and Flx-treated WT mice. d PMA (200 nM) decreased the amplitude of KV currents in Flx-treated mice ( n = 4, 3) but not Veh-treated WT mice ( n = 3, 3). RM one-way ANOVA, * P < 0.05. e Model for serotonergic signaling in PV hippocampal cells. Left: initial exposure to SSRIs does not alter cAMP levels and PKA activity. PP2A activity remains high and Kv3.1β is dephosphorylated. Right: chronic SSRIs increases 5-HT5A surface levels. 5-HT5AR activation reduces cAMP levels and diminishes PKA and PP2A activities to reduce Ser 503 Kv3.1β dephosphorylation
Protein Product Number Vendor, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/protein product number vendor/product/Rockland Immunochemicals
Average 90 stars, based on 1 article reviews
protein product number vendor - by Bioz Stars, 2026-05
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Image Search Results


5-HT5A signaling in PV neurons. a Bar graph summary of cAMP level in N2A cells transiently transfected with mouse 5-HT5A. The cells were incubated with the indicated amounts of forskolin and 5-HT. One representative experiment out of three is shown. Bars represent means of cAMP level ( n = 4 wells per group) as percentage of baseline + SD. One Way ANOVA, * P < 0.05 vs. baseline. b Representative western blot images (top) and bar graph summary (bottom) depicting the levels of Kv3.1β and Ser503 pKv3.1β in transfected HEK293 cells treated as indicated ( n = 4 wells per condition). Bars represent mean densities of the pKv normalized to that of the total Kv. Number and arrowhead indicate protein size in kDa. One-way ANOVA. * P < 0.05, ** P < 0.01. OA okadaic acid, PMA Phorbol 12-myristate 13-acetate. c Representative traces of Kv potassium currents evoked with 10 mV potential steps from −70 to +50 mV in PV DG neurons from Veh- and Flx-treated WT mice. d PMA (200 nM) decreased the amplitude of KV currents in Flx-treated mice ( n = 4, 3) but not Veh-treated WT mice ( n = 3, 3). RM one-way ANOVA, * P < 0.05. e Model for serotonergic signaling in PV hippocampal cells. Left: initial exposure to SSRIs does not alter cAMP levels and PKA activity. PP2A activity remains high and Kv3.1β is dephosphorylated. Right: chronic SSRIs increases 5-HT5A surface levels. 5-HT5AR activation reduces cAMP levels and diminishes PKA and PP2A activities to reduce Ser 503 Kv3.1β dephosphorylation

Journal: Molecular Psychiatry

Article Title: Emergence of 5-HT5A signaling in parvalbumin neurons mediates delayed antidepressant action

doi: 10.1038/s41380-019-0379-3

Figure Lengend Snippet: 5-HT5A signaling in PV neurons. a Bar graph summary of cAMP level in N2A cells transiently transfected with mouse 5-HT5A. The cells were incubated with the indicated amounts of forskolin and 5-HT. One representative experiment out of three is shown. Bars represent means of cAMP level ( n = 4 wells per group) as percentage of baseline + SD. One Way ANOVA, * P < 0.05 vs. baseline. b Representative western blot images (top) and bar graph summary (bottom) depicting the levels of Kv3.1β and Ser503 pKv3.1β in transfected HEK293 cells treated as indicated ( n = 4 wells per condition). Bars represent mean densities of the pKv normalized to that of the total Kv. Number and arrowhead indicate protein size in kDa. One-way ANOVA. * P < 0.05, ** P < 0.01. OA okadaic acid, PMA Phorbol 12-myristate 13-acetate. c Representative traces of Kv potassium currents evoked with 10 mV potential steps from −70 to +50 mV in PV DG neurons from Veh- and Flx-treated WT mice. d PMA (200 nM) decreased the amplitude of KV currents in Flx-treated mice ( n = 4, 3) but not Veh-treated WT mice ( n = 3, 3). RM one-way ANOVA, * P < 0.05. e Model for serotonergic signaling in PV hippocampal cells. Left: initial exposure to SSRIs does not alter cAMP levels and PKA activity. PP2A activity remains high and Kv3.1β is dephosphorylated. Right: chronic SSRIs increases 5-HT5A surface levels. 5-HT5AR activation reduces cAMP levels and diminishes PKA and PP2A activities to reduce Ser 503 Kv3.1β dephosphorylation

Article Snippet: Staining and analysis of 5-HT5A and Ser503 phospho Kv3.1β (pKv3.1β) were done for all samples at the same time using commercial antibodies against 5-HT5A (LifeSpan Biosciences, LS-A2119), Ser503 phospho Kv3.1β (Phosphosolutions, p1550-503), and PV (Sigma Aldrich, P0388).

Techniques: Transfection, Incubation, Western Blot, Activity Assay, Activation Assay, De-Phosphorylation Assay