Journal: Journal of Proteomics

Article Title: Vitamin D binding protein isoforms as candidate predictors of disease extension in childhood arthritis

doi: 10.1016/j.jprot.2012.06.024

Figure Lengend Snippet: Verification of protein expression and localization in synovial fluid and membrane. A. Representative MALDI-TOF mass spectra of spot 873 identified as vitamin D-binding protein. The matched peptide sequences are underlined within the sequence of vitamin D-binding protein. An approximate molecular weight of the protein cleavage product (between the arrows) is calculated from the amino acid sequence, in line with DIGE estimate. B. Immunoprecipitation of VDBP from synovial fluids of n = 48 representative JIA patients (indicated by number in lanes 1–48; M — molecular weight marker; D — purified human VDBP positive control). Band densities of vitamin D binding protein concur with protein expression levels measured by DIGE (extended-to-be oligoarticular patients in lanes 13, 21, 24, 36, 37, 45, 47, 48). C. Representative immunohistochemistry of neighboring sections of synovial membrane from a polyarticular patient, all captured at 10 × magnification. Vitamin D binding protein expression is perivascular in nature. D. ELISA quantification of c-reactive protein and vitamin D binding protein concentration in initial plasma and synovial samples taken from the whole study cohort. Error bars on the box-whisker plots represent range between maximum and minimum values; center line represents median.

Article Snippet: A purified form of human VDBP was run with IP samples as a positive control (2 μg) (Athens Research & Technology Inc., Athens, GA, USA).

Techniques: Expressing, Membrane, Binding Assay, Sequencing, Molecular Weight, Immunoprecipitation, Marker, Purification, Positive Control, Immunohistochemistry, Enzyme-linked Immunosorbent Assay, Protein Concentration, Clinical Proteomics, Whisker Assay

Journal: Experimental biology and medicine (Maywood, N.J.)

Article Title: Concentrated fish oil (Lovaza ® ) extends lifespan and attenuates kidney disease in lupus-prone short-lived (NZBxNZW)F1 mice

doi: 10.1177/1535370213489485

Figure Lengend Snippet: Composition of AIN-93 semi-purified diets containing placebo, Lovaza ® and fish oil-18/12

Article Snippet: In phase 2, a set of mice ( n =6/group) were sacrificed after six months of dietary interventions for the evaluation of serum, molecular, histological and enzymatic assays as the dose-dependent and comparative output measurements of dietary interventions table ft1 table-wrap mode="anchored" t5 caption a7 Diet Ingredients * 1% Placebo 4% Placebo 1% Lovaza ® 4% Lovaza ® 4% FO-18/12 Casein 14.00 14.00 14.00 14.00 14.00 Corn starch 50.43 47.43 50.43 47.43 42.73 Dextronized corn starch 14.50 14.50 14.50 14.50 14.50 Sucrose 9.00 9.00 9.00 9.00 9.00 Cellulose 5.00 5.00 5.00 5.00 5.00 AIN-93 mineral mix 3.50 3.50 3.50 3.50 3.50 AIN-93 vitamin mix 1.00 1.00 1.00 1.00 1.00 l-cystine 0.18 0.18 0.18 0.18 0.18 Choline bitartrate 0.25 0.25 0.25 0.25 0.25 TBHQ 0.10 0.10 0.10 0.10 0.10 Vitamin E 0.04 0.04 0.04 0.04 0.04 † Lovaza ® 0 0 1 4 0 Placebo/Corn oil 2 5 1 1 1 ‡ Flsh oil -18/12 0 0 0 0 4 Open in a separate window * AII diet ingredients were purchased from MP Biomedicals (Irvine, CA).

Techniques:

Journal:

Article Title: Yogurt protects against growth retardation in weanling rats fed diets high in phytic acid

doi: 10.1016/j.jnutbio.2008.12.005

Figure Lengend Snippet: Composition of AIN-76 Basal Diet

Article Snippet: Diets were prepared such that calorie and zinc content were not significantly different. table ft1 table-wrap mode="anchored" t5 caption a7 Ingredient % Sucrose 50.0 Casein Purified High Nitrogen 20.0 Corn Starch 15.0 Fiber (Alphacel non-nutritive bulk) 5.0 Corn Oil 5.0 AIN-76 Mineral Mix 2 3.5 AIN-76 Vitamin Mix 3 1.0 DL-Methionine 0.3 Choline Bitartrate 0.2 Open in a separate window 1 ICN Nutritional Biochemicals/MP Biomedicals, Solon, OH 2 Mineral Mixture (g/kg of mixture): Calcium Phosphate Dibasic 500, Potassium Citrate Monohydrate 220, Sodium Chloride 74, Potassium Sulfate 52, Magnesium Oxide 24, Ferric Citrate(16–17% Fe) 6 Manganous Carbonate(43–48% Mn) 3.5, Zinc Carbonate (70% ZnO) 1.6, Chromium Potassium Sulfate 0.55, Cupric Carbonate (53–55% Cu) 0.3, Potassium Iodate 0.01, Sodium Selenite 0.01, Sucrose, finely powdered 118.

Techniques: Purification