Journal: EMBO Molecular Medicine

Article Title: Inhibition of NPC1L1 disrupts adaptive responses of drug‐tolerant persister cells to chemotherapy

doi: 10.15252/emmm.202114903

Figure Lengend Snippet: Chemical reagents in this study.

Article Snippet: Alpha‐Vitamin E , S4686, Selleck.

Techniques:

Journal: Cancers

Article Title: Optimization of a Luteolin-Loaded TPGS/Poloxamer 407 Nanomicelle: The Effects of Copolymers, Hydration Temperature and Duration, and Freezing Temperature on Encapsulation Efficiency, Particle Size, and Solubility

doi: 10.3390/cancers15143741

Figure Lengend Snippet: Encapsulation efficiency (EE) of Lut-loaded TPGS/Pol micelle with different concentrations of co-polymers and ratios of TPGS/Pol (V:P).

Article Snippet: Luteolin and vitamin E TPGS (tocofersolan) was purchased from MedChem Express (Monmouth Junction, NJ, USA), Poloxamer 407 was purchased from Sigma-Aldrich (St. Louis, MO, USA) and ethanol was purchased from R&M Chemicals Sdn Bhd (Subang, Malaysia).

Techniques: Encapsulation

Journal: Cancers

Article Title: Optimization of a Luteolin-Loaded TPGS/Poloxamer 407 Nanomicelle: The Effects of Copolymers, Hydration Temperature and Duration, and Freezing Temperature on Encapsulation Efficiency, Particle Size, and Solubility

doi: 10.3390/cancers15143741

Figure Lengend Snippet: Fourier-transform infrared (FTIR) spectrum of ( A ) Lut, ( B ) TPGS, ( C ) Pol, and ( D ) sample.

Article Snippet: Luteolin and vitamin E TPGS (tocofersolan) was purchased from MedChem Express (Monmouth Junction, NJ, USA), Poloxamer 407 was purchased from Sigma-Aldrich (St. Louis, MO, USA) and ethanol was purchased from R&M Chemicals Sdn Bhd (Subang, Malaysia).

Techniques: Fourier Transform Infrared Spectroscopy

Journal: Cancers

Article Title: Optimization of a Luteolin-Loaded TPGS/Poloxamer 407 Nanomicelle: The Effects of Copolymers, Hydration Temperature and Duration, and Freezing Temperature on Encapsulation Efficiency, Particle Size, and Solubility

doi: 10.3390/cancers15143741

Figure Lengend Snippet: Crystallinity analysis of ( A ) Lut, ( B ) Pol, ( C ) TPGS, ( D ) blank micelle and ( E ) Lut-loaded micelle via XRD.

Article Snippet: Luteolin and vitamin E TPGS (tocofersolan) was purchased from MedChem Express (Monmouth Junction, NJ, USA), Poloxamer 407 was purchased from Sigma-Aldrich (St. Louis, MO, USA) and ethanol was purchased from R&M Chemicals Sdn Bhd (Subang, Malaysia).

Techniques:

Journal: Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry

Article Title: Substrate Binding Switches the Conformation at the Lynchpin Site in the Substrate-Binding Domain of Human Hsp70 to Enable Allosteric Interdomain Communication

doi: 10.3390/molecules23030528

Figure Lengend Snippet: PRE experiments with SBD(∆CDE)-y with spin labeling at residue 537. ( a ) The spin-labeling position in the solution structure of SBD(∆CDE)-y is shown, in which, S537 was exchanged to cysteine to facilitate covalent labeling with MTSL; ( b ) Representative spectral changes caused by the paramagnetic effect. In the left two panels, 2D 1 H- 15 N HSQC spectra are overlaid for the paramagnetic spin labeled sample (red) and for the sample with a diamagnetic label (black) in the absence of the NR-peptide. In the two panels on the right, the corresponding spectral comparison in the presence of 5.0 mM NR-peptide. The concentration of SBD(∆CDE)-y was adjusted to 0.5 mM.

Article Snippet: The SBD(∆CDE) (S537C/L542Y) mutant was incubated with 10-fold molar excess of (1-oxyl-2,2,5,5-tetramethyl-delta(3)-pyrroline-3-methyl)-methanethiosulfonate (MTSL), or a diamagnetic control MTSL analog, (1-acetoxy-2,2,5,5-tetramethyl-δ-3-pyrroline-3-methyl)methane-thiosulfonate (Toronto Research Chemicals, Toronto, ON, Canada), dissolved in DMSO at 296 K (23 °C) for 10 h. The sample solution was dialyzed extensively against buffer E at 277 K (4 °C) to remove unreacted MTSL.

Techniques: Labeling, Residue, Comparison, Concentration Assay