Journal: Molecular Medicine Reports

Article Title: Growth differentiation factor-5 induces tenomodulin expression via phosphorylation of p38 and promotes viability of murine mesenchymal stem cells from compact bone

doi: 10.3892/mmr.2017.8325

Figure Lengend Snippet: GDF-5 treatment increased the protein expression levels of TNMD via phosphorylation of p38 in MSCs from murine compact bone. (A) MSCs from murine compact bone were treated with 100 ng/ml GDF-5 for 0, 5, 10, 30 and 60 min, and 6 and 24 h. Phosphorylation of p38 was determined by western blotting. (B and C) MSCs from murine compact bone were pretreated with 10 µM SB203580 (an inhibitor of p38) for 1 h, and subsequently treated with 100 ng/ml GDF-5 for 30 min. (B) Phosphorylation of p38 and (C) TNMD expression were determined by western blotting. MSCs, mesenchymal stem cells; GDF-5, growth differentiation factor-5; TNMD, tenomodulin; p-p38, phosphorylated-p38.

Article Snippet: Cells were synchronized and pre-stimulated with 10 μM SB203580 (Tocris Bioscience, Bristol, UK; an inhibitor of p38 MAPK) at 37°C for 1 h. The control group was treated with an equivalent volume of 0.1% dimethyl sulfoxide.

Techniques: Expressing, Phospho-proteomics, Western Blot

Journal: Frontiers in Physiology

Article Title: Obesity Mediates Apoptosis and Extracellular Matrix Metabolic Imbalances via MAPK Pathway Activation in Intervertebral Disk Degeneration

doi: 10.3389/fphys.2019.01284

Figure Lengend Snippet: (A) Western Blot of MAPK pathway protein detection: P38 and ERK. (B) The results of real-time PCR for Aggrecan, Collagen-II, MMP-3, and MMP-13 using RNA from nucleus pulposus cells initiated by different P38 inhibitor (SB203580) concentrations and PA solution. (C) Apoptotic nucleus pulposus cells detection by flow cytometry. a:0 μmol/L PA + 0 μmol/L SB203580, b:150 μmol/L PA + 0 μmol/L SB203580, c:150 μmol/L PA + 1 μmol/L SB203580, d:150 μmol/L PA + 10 μmol/L SB203580, e:150 μmol/L PA + 20 μmol/L SB203580, f:150 μmol/L PA + 30 μmol/L SB203580. (D) Statistics analysis of apoptotic cells in upper right (UP) and low right (LR) regions.

Article Snippet: Cell signaling following PA stimulation was primarily via the MAPK signal pathway, especially the ERK pathway was involved in NP cell apoptosis.

Techniques: Western Blot, Real-time Polymerase Chain Reaction, Flow Cytometry

Journal: iScience

Article Title: Multi-omics analysis of a drug-induced model of bipolar disorder in zebrafish

doi: 10.1016/j.isci.2023.106744

Figure Lengend Snippet: MAPK/JNK-mediated neuroinflammation in BD zebrafish (A) Heatmap shows overall changes in genes related to the MAPK/JNK signaling pathway. (B) Quantitative analysis of genes related to the MAPK/JNK signaling pathway using qPCR. (C and D) Expression of GFAP, P-JNK and JNK in CON and BD was detected by Western blot. ∗p < 0.05, unpaired two-tailed Student’s t test, data are shown as mean ± SEM. (E) Representative immunofluorescence images of midbrain after staining for GFAP (astrocyte, red), Iba-1 (microglia, red), NeuN (neurons, green), and DAPI (all nuclei, blue). Scale bar = 100 μm. (F) Quantitative analysis of pro-inflammatory cytokine levels using ELISA. ∗p < 0.05, unpaired two-tailed Student’s t test, data are shown as mean ± SEM (CON and BD, n = 3).

Article Snippet: Proteins were detected using the following primary antibodies: GFAP (Proteintech, Cat. #16825-1-AP), NeuN (Proteintech, Cat. #26975-1-AP), Iba-1 (Proteintech, Cat. #10904-1-AP), Synaptophysin (Abcam, Cat. #ab32594), β-Actin (Proteintech, Cat. #66009-1-IG), Anti-JNK1 + JNK2 + JNK3 (Abcam, Cat. #ab179461), Phospho-SAPK/JNK (Cell Signalling Technology, Cat. #4668T), p38 MAPK (Proteintech, Cat. #14064-1-AP) and Phospho-p38 MAPK (Cell Signalling Technology, Cat. #4511T).

Techniques: Expressing, Western Blot, Two Tailed Test, Immunofluorescence, Staining, Enzyme-linked Immunosorbent Assay

Journal: iScience

Article Title: Multi-omics analysis of a drug-induced model of bipolar disorder in zebrafish

doi: 10.1016/j.isci.2023.106744

Figure Lengend Snippet:

Article Snippet: Proteins were detected using the following primary antibodies: GFAP (Proteintech, Cat. #16825-1-AP), NeuN (Proteintech, Cat. #26975-1-AP), Iba-1 (Proteintech, Cat. #10904-1-AP), Synaptophysin (Abcam, Cat. #ab32594), β-Actin (Proteintech, Cat. #66009-1-IG), Anti-JNK1 + JNK2 + JNK3 (Abcam, Cat. #ab179461), Phospho-SAPK/JNK (Cell Signalling Technology, Cat. #4668T), p38 MAPK (Proteintech, Cat. #14064-1-AP) and Phospho-p38 MAPK (Cell Signalling Technology, Cat. #4511T).

Techniques: Recombinant, Protein Extraction, Bicinchoninic Acid Protein Assay, Enzyme-linked Immunosorbent Assay, Sequencing, Software