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Image Search Results
Journal: bioRxiv
Article Title: RSV Infects the Human Nasal Epithelium via the Basolateral Route with Distinct Subgroup Infectivity and Basal Cell Tropism
doi: 10.64898/2026.03.05.709813
Figure Lengend Snippet: (A) Apically released plaque forming units of RSV/B/BA from each transwell replicate of one adult HNO-ALI line at 5 and 8 dpi. LOD = limit of detection. (B) Distribution of cell populations from basolateral mock and RSV/B/BA inoculation at 5 and 8 dpi. (C) Representative spectral flow cytometry plots showing gating strategy to identify Krt5+, Krt23+, and Ace-tub+ cell populations in basolateral mock and RSV/B/BA inoculated HNO-ALIs at 5 dpi. Further gating of RSV F protein was used to determine RSV infection in each cell population. (D) Pie charts summarizing the average proportion of each cell population in basolateral mock and RSV/B/BA inoculated HNO-ALIs at 5 dpi. (E) Pie charts summarizing RSV/B/BA infection in each cell population at 5 dpi.
Article Snippet: Immunostaining for intracellular proteins was performed using the following primary conjugated antibodies: Krt5 (1:5000, Abcam, Catalog number: ab224985), Krt17 (1:100, Abcam, Catalog number: ab185032), Krt23 (1:100, Novus Biologicals, Catalog number: NBP2–22590AF488), acetylated α-tubulin (1:100, Santa Cruz, Catalog number: sc-23950 AF594), and
Techniques: Flow Cytometry, Infection