Journal: International Journal of Molecular Medicine

Article Title: Apelin-13 in the paraventricular nucleus (PVN) attenuates myocardial ischemia through V1a receptors in PVN/nucleus tractus solitarii (NTS) and GARγ2 in NTS

doi: 10.3892/ijmm.2025.5652

Figure Lengend Snippet: Effects of apelin-13 microinjection into the PVN on cardiac function in MI model rats. (A) MI models were established one day after implanting an osmotic mini-pump delivering apelin-13 into the PVN. Cardiac function was assessed and tissue samples were collected 28 days after mini-pump implantation. (B) Representative western blots for the V1a receptor and GARγ2 in the PVN and NTS from MI model rats and MI model rats continuously microinjected with apelin-13 into the PVN for 28 days. (C) Quantification of V1a receptor protein levels. (D) Quantification of GARγ2 receptor protein levels. (E) Representative ultrasound images (M-mode) performed on days 28 after MI and sham-operated control. Echocardiographic results for (F) LVEDD, (G) LVESD, (H) LVEF and (I) LVFS (n=6 in the sham-operated control group; n=5 in the MI group, one rat succumbed at 20 days; n=6 in the MI + apelin group). Plasma levels of (J) SST, (K) CCK, (L) VIP, (M) GLP-1, (N) noradrenaline and (O) vasopressin (n=6 in the sham-operated control group; n=5 in the MI group, one rat succumbed at 20 days; n=6 in the MI + apelin group). Normality was tested using the Shapiro-Wilk test. The data in C-D show mean ± SEM; F-O show mean ± SD. Statistical significance was determined by one-way ANOVA, followed by Tukey's multiple-comparisons test. * P<0.05; ** P<0.01; *** P<0.001. Post-hoc power exceeded 80% for all comparisons. PVN, paraventricular nucleus; MI, myocardial infarction; V1a, Vasopressin 1a; GAR, GABA A receptor; NTS, nucleus tractus solitarii; LVEDD, left ventricular end-diastolic diameter; LVESD, lower left ventricular end-systolic diameter; LVEF, left ventricular ejection fraction; LVFS, left ventricular fraction shortening; SST, isomatostatin; CCK, cholecystokinin; VIP, vasoactive intestinal peptide; GLP-1, glucagon-like peptide 1.

Article Snippet: The levels of somatostatin, cholecystokinin, glucagon-like peptide-1 and vasoactive intestinal peptide in the serum were quantified using enzyme-linked immunosorbent assay (ELISA) kits: Somatostatin (cat. no. NBP2-80271; Novus Biologicals; Bio-Techne), cholecystokinin (cat. no. EIAR-CCK-1; RayBiotech, Inc.), glucagon-like peptide-1 (cat. no. E-EL-R3007-96T; Wuhan Elabscience Biotechnology Co., Ltd. Wuhan Elabscience Biotechnology Co., Ltd.), and vasoactive intestinal peptide (cat. no. NBP2-82466; Novus Biologicals; Bio-Techne).

Techniques: Microinjection, Western Blot, Control, Clinical Proteomics

Journal: International Journal of Molecular Medicine

Article Title: Apelin-13 in the paraventricular nucleus (PVN) attenuates myocardial ischemia through V1a receptors in PVN/nucleus tractus solitarii (NTS) and GARγ2 in NTS

doi: 10.3892/ijmm.2025.5652

Figure Lengend Snippet: Mechanisms of the effect of apelin-13 overexpression (AAV2-apelin-13 gene transfer) in the PVN on V1a receptor-mediated improvement in cardiac function in MI model rats. (A) Representative western blot image of V1a receptor expression in the PVN or NTS and GARγ2 expression in the NTS. (B-D) Quantification of V1a receptor expression. (E) Representative western blotting to assess Bcl-2, Bax, TGF-β1 and Smad2 protein levels in the heart and (F-I) quantification of the protein levels. Plasma levels of (J) SST, (K) CCK, (L) VIP and (M) GLP-1 (n=7 in the sham-operated control group; n=5 in the MI group, 2 rats succumbed; n=7 in the MI + apelin-13 group; n=5 in the MI + apelin-13 + SR49059 (PVN) group, 2 rats succumbed; n=6 in the MI + apelin-13 + SR49059 (NTS) group, 1 rat succumbed; n=6 in the MI + apelin-13 + muscimol (NTS) group, 1 rat succumbed). Representative plasma levels of (N) noradrenaline and (O) vasopressin (n=7 in the sham-operated control group; n=6 in the MI group, 1 rat succumbed; n=7 in the MI + apelin-13 group; n=6 in the MI + apelin-13 + SR49059 (PVN) group, 1 rat succumbed; n=6 in the MI + apelin-13 + SR49059 (NTS) group, 1 rat succumbed; n=7 in the MI + apelin-13 + muscimol (NTS) group). Normality was tested using the Shapiro-Wilk test. The data in B-D and F-I show mean ± SEM; data in J-O are shown as mean ± SD and were analyzed via one-way ANOVA, followed by Tukey's multiple-comparisons test. In B-D and F-O, * P<0.05 vs. sham-operated control group; † P<0.05 vs. MI group; ‡ P<0.05 vs. MI + apelin-13 group; § P<0.05 vs. MI + apelin-13 + SR49059 (PVN); # P<0.05 vs. MI + apelin-13 + SR49059 (NTS). Post-hoc power exceeded 80% for all key comparisons. PVN, paraventricular nucleus; V1a, Vasopressin 1a; MI, myocardial infarction; NTS, nucleus tractus solitarii; GAR, GABA A receptor; SST, isomatostatin; CCK, cholecystokinin; VIP, vasoactive intestinal peptide; GLP-1, glucagon-like peptide 1.

Article Snippet: The levels of somatostatin, cholecystokinin, glucagon-like peptide-1 and vasoactive intestinal peptide in the serum were quantified using enzyme-linked immunosorbent assay (ELISA) kits: Somatostatin (cat. no. NBP2-80271; Novus Biologicals; Bio-Techne), cholecystokinin (cat. no. EIAR-CCK-1; RayBiotech, Inc.), glucagon-like peptide-1 (cat. no. E-EL-R3007-96T; Wuhan Elabscience Biotechnology Co., Ltd. Wuhan Elabscience Biotechnology Co., Ltd.), and vasoactive intestinal peptide (cat. no. NBP2-82466; Novus Biologicals; Bio-Techne).

Techniques: Over Expression, Western Blot, Expressing, Clinical Proteomics, Control

Journal: International Journal of Molecular Medicine

Article Title: Apelin-13 in the paraventricular nucleus (PVN) attenuates myocardial ischemia through V1a receptors in PVN/nucleus tractus solitarii (NTS) and GARγ2 in NTS

doi: 10.3892/ijmm.2025.5652

Figure Lengend Snippet: Graphical model summarizing the hypothesized pathway. Overexpression of apelin-13 in the PVN promotes upregulation of V1a receptors in both the PVN and NTS, while downregulating GARγ2 in the NTS. These neural modifications collectively enhance parasympathetic outflow. Furthermore, paraventricular endocrine system secretes circulating neurohumoral factors that systemically mediate cardioprotection. PVN, paraventricular nucleus; V1a, Vasopressin 1a; MI, myocardial infarction; NTS, nucleus tractus solitarii; GAR, GABA A receptor; SST, isomatostatin; CCK, cholecystokinin; GLP-1, glucagon-like peptide 1; VIP, vasoactive intestinal peptide.

Article Snippet: The levels of somatostatin, cholecystokinin, glucagon-like peptide-1 and vasoactive intestinal peptide in the serum were quantified using enzyme-linked immunosorbent assay (ELISA) kits: Somatostatin (cat. no. NBP2-80271; Novus Biologicals; Bio-Techne), cholecystokinin (cat. no. EIAR-CCK-1; RayBiotech, Inc.), glucagon-like peptide-1 (cat. no. E-EL-R3007-96T; Wuhan Elabscience Biotechnology Co., Ltd. Wuhan Elabscience Biotechnology Co., Ltd.), and vasoactive intestinal peptide (cat. no. NBP2-82466; Novus Biologicals; Bio-Techne).

Techniques: Over Expression

Journal: Immunity, inflammation and disease

Article Title: Vasoactive intestinal peptide exerts therapeutic action by regulating PTEN in a model of Sjögren's disease.

doi: 10.1002/iid3.936

Figure Lengend Snippet: FIGURE 3 Vasoactive intestinal peptide (VIP) upregulated PTEN, VIP receptor mRNA expression, and Treg‐related cytokines in spleen. Q‐PCR results for VIP mRNA, VIP receptors mRNA expression (A), and PTEN/PI3K/AKT pathway (B) in the spleen. The concentrations of VIP in the serum (C) were measured using ELISA kits (n = 7). The abundance of interleukin (IL)‐10 (D) and IL‐2 (E) in the splenic tissue homogenate supernatant was determined by ELISA as well (n = 7). Unpaired Student t‐test was used in the analysis. *p < 0.05, **p < 0.01, ***p < 0.001.

Article Snippet: Serum extraction method as published,20 VIP levels in serum were measured using the mouse VIP ELISA kit (Elabscience).

Techniques: Expressing, Enzyme-linked Immunosorbent Assay

Journal: Communications Biology

Article Title: Anterior cingulate cortex mediates the comorbidity between colorectal cancer and depression-like behaviors

doi: 10.1038/s42003-025-08719-z

Figure Lengend Snippet: A Representative images of parasympathetic nerve markers VAChT staining in the colorectal tissue. VAChT (Green) and DAPI (blue). B Representative images of sympathetic nerve markers TH staining in the colorectal tissue. TH (Green) and DAPI (blue). Scale bar: 100 μm. C Quantitative of the density of VAChT-labeled parasympathetic nerve in the colorectal tissue across groups (n = 5,8,12,6 per group). D Quantitative of the density of TH-labeled sympathetic nerve in the colorectal tissue across groups (n = 5,10,12,6 per group). E Representative images of sensory nerve markers CGRP staining in the colorectal tissue. CGRP (Green) and DAPI (blue). F Representative images of peptiderergic nerve markers VIP staining in the colorectal tissue. VIP (Green) and DAPI (blue). Scale bar: 100 μm. G Quantitative of the density of CGRP-labeled sensory nerve in the colorectal tissue across groups (n = 4,10,8,6 per group). H Quantitative of the density of VIP-labelled peptiderergic nerve in the colorectal tissue across groups (n = 5,8,11,6 per group). I Representative images of cell proliferation markers Ki67 staining in the colorectal tumor tissue. Ki67 (Green) and DAPI (blue). Scale bar: 100 μm. J Representative images of apoptosis markers Cleaved-caspase3 staining in the colorectal tumor tissue. Cleaved-caspase3 (Green) and DAPI (blue). Scale bar: 100 μm. K Quantitative of the fluorescence intensity of the Ki67 in the colorectal tumor tissue across groups (n = 10,10,6 per group). L Quantitative of the fluorescence intensity of the Cleaved-caspase3 in the colorectal tumor tissue across groups (n = 11,10,5 per group). M Representative Western blot images showing the expression of Ki-67, PCNA, and cleaved caspase-3 in tumor tissues from CRC+Ctrl-ACC (Ctrl), CRC+Gi-ACC (Gi), and CRC+Gq-ACC (Gq) mice. N – P Quantification of Ki-67, PCNA, and cleaved caspase-3 protein levels normalized to β-Tublin in each group, (n = 5,5,6 per group; n = 5,5,6 per group, n = 5,6,6 per group). Q Representative images of tumor size in CRC+Ctrl-ACC, CRC+Gi-ACC and CRC+Gq-ACC groups. R Quantification of tumor volume in CRC+Ctrl-ACC, CRC+Gi-ACC and CRC+Gq-ACC groups, (n = 5,6,6 per group). All data are presented as mean ± s.e.m of the fold change of the Sham+Ctrl-ACC group and analyzed by one-way ANOVA followed by Tukey’s multiple comparisons test ( C , D , G , H ). All data are presented as mean ± s.e.m of the fold change of the CRC+Ctrl-ACC group and analyzed by one-way ANOVA followed by Tukey’s multiple comparisons test ( K , L , N – P , R ). The source data are provided as Supplementary Data in the Figshare repository.

Article Snippet: After sealing at room temperature for 2 h, tip off the blocking solution, drain the excess water on the filter paper pad, place it flat in the wet box, add 200 μL primary antibody (diluted in the blocking buffer) for VAChT (Synaptic Systems, Germany; cat#139103; 1:500), TH (Cell Signaling Technology, USA; cat#E2L6M; 1:500), CGRP (Cell Signaling Technology, USA; cat#14959; 1:500), VIP (Cell Signaling Technology, USA; cat#63269; 1:500), Ki67 (Cell Signaling Technology, USA; cat#9129; 1:500) and cleaved caspase-3 (Cell Signaling Technology, USA; cat# 9664; 1:500) and incubate at 4 °C for 12 h, and then incubate at room temperature for 4 h. On the second day, the colorectal frozen sections were rinsed with 0.4% PBST three times at room temperature for 10 min each time.

Techniques: Staining, Labeling, Fluorescence, Western Blot, Expressing