Journal: iScience

Article Title: Increased SIRT3 combined with PARP inhibition rescues motor function of SBMA mice.

doi: 10.1016/j.isci.2023.107375

Figure Lengend Snippet: Figure 1. SIRT3 and SOD2 proteins are reduced in AR100Q mice, and activating and increasing SIRT3 reduces ROS and cell death in vitro (A) Western analysis of quadriceps from 11 week-old WT, AR24Q, and AR100Q male mice (n = 3). Top, total SOD2 with tubulin loading control, and densitometry analysis of total SOD2 compared to tubulin. Bottom, a second SDS-PAGE gel probed for acetylated (acetyl-K68) SOD2 with tubulin loading control, and densitometry analysis of acetyl-K68/total SOD2 (from A top) after each were normalized to tubulin loading control. (B) Western analysis of quadriceps from 11 week-old WT, AR24Q, and AR100Q male mice (n = 3). Endogenous SIRT3 and tubulin loading control with densitometry analysis of SIRT3 normalized to tubulin loading control. (C) ROS levels in AR112Q expressing PC12 cells (n = 12) treated with 10 nM DHT plus either DMSO (vehicle control), 1 mM viniferin, 1 mM honokiol, or 100 mM melatonin for 48 h (representative image from 3 experiments).

Article Snippet: Cells were treated with honokiol (Tocris), viniferin (Carbosynth), melatonin (Tocris), olaparib (Selleck Chemicals), or a DMSO control for 48 or 96 h before undergoing specific assays.

Techniques: In Vitro, Western Blot, Control, SDS Page, Expressing

Journal: Plants

Article Title: The Influence of the Grapevine Bacterial and Fungal Endophytes on Biomass Accumulation and Stilbene Production by the In Vitro Cultivated Cells of Vitis amurensis Rupr.

doi: 10.3390/plants10071276

Figure Lengend Snippet: The content of stilbenes in the Vitis amurensis cell culture V7 after 3 days of cocultivation with endophytic bacteria or fungi, mg/g DW. * p < 0.05; ** p < 0.01 versus values of stilbene accumulation in the V7 cell cultivated under control conditions without bacteria and fungi.

Article Snippet: The analytical standards trans -resveratrol, trans -piceid, and trans -piceatannol were obtained from Sigma-Aldrich (St. Louis, MO, USA), and d -viniferin was obtained from Panreac AppliChem (GmbH, Darmstadt, Germany).

Techniques: Cell Culture, Bacteria, Control

Journal: Scientific Reports

Article Title: Stability testing of resveratrol and viniferin obtained from Vitis vinifera L. by various extraction methods considering the industrial viewpoint

doi: 10.1038/s41598-020-62603-w

Figure Lengend Snippet: Chromatogram of all three stilbenes in full-scan (negative mode with electrospray ionization). 1: trans -resveratrol; 2: cis -ε-viniferin; 3: trans -ε-viniferin; 4: r2-viniferin.

Article Snippet: The trans -resveratrol standard (99%) along with commercialized standard of trans -ε-viniferin (95%) (Fig. ) were purchased from Sigma-Aldrich (Prague, Czech Republic), while the cis -ε-viniferin was generated from trans -ε-viniferin standard by UV exposition (Spectroline, CV-10, New York, USA).

Techniques:

Journal: Scientific Reports

Article Title: Stability testing of resveratrol and viniferin obtained from Vitis vinifera L. by various extraction methods considering the industrial viewpoint

doi: 10.1038/s41598-020-62603-w

Figure Lengend Snippet: ( a ) trans -resveratrol, ( b ) cis -ε-viniferin, and ( c ) trans -ε-viniferin - Comparison of maceration (MAC) carried out for 1, 3, 7, and 14 days and Soxhlet extraction (SOX) for 3 hours, both in light and dark with respect to the yield of desirable components (white column), mg/g plant and their concentration in extracts (black column), wt.% (based on total mass of extract) with 95% confidence intervals.

Article Snippet: The trans -resveratrol standard (99%) along with commercialized standard of trans -ε-viniferin (95%) (Fig. ) were purchased from Sigma-Aldrich (Prague, Czech Republic), while the cis -ε-viniferin was generated from trans -ε-viniferin standard by UV exposition (Spectroline, CV-10, New York, USA).

Techniques: Concentration Assay