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Image Search Results
Journal: Frontiers in pharmacology
Article Title: Autophagic Inhibition of Caveolin-1 by Compound Phyllanthus urinaria L. Activates Ubiquitination and Proteasome Degradation of β-catenin to Suppress Metastasis of Hepatitis B-Associated Hepatocellular Carcinoma.
doi: 10.3389/fphar.2021.659325
Figure Lengend Snippet: FIGURE 2 | CP inhibited migration and invasion of HBV-associated HCC. (A) HBV-associated cells including HepG2-HBx and HepG2-URG11 following CP administration and sorafenib treatment exhibited a significant delay in wound healing compared with that of control group. (B) CP treatment remarkably decreased the number of HBV-associated cells that migrated across matrigel and transwell chamber. (C) Western blot analysis showed that CP dramatically decreased expression levels of Vimentin and N-cadherin and increased E-cadherin level in both HepG2-HBx and HepG2-URG11 cells in a time-concentration dependent manner. All values represented the means ± SD (n 3, *p < 0.05, **p < 0.01, ***p < 0.001).
Article Snippet: Primary antibodies used in our study contained Cav1(16447-1-AP, Proteintech, Chicago, United States), N-cadherin (22018-1-AP, Proteintech, Chicago, United States), E-cadherin (20874-1-AP, Proteintech, Chicago, United States),
Techniques: Migration, Control, Western Blot, Expressing, Concentration Assay
Journal: Frontiers in pharmacology
Article Title: Autophagic Inhibition of Caveolin-1 by Compound Phyllanthus urinaria L. Activates Ubiquitination and Proteasome Degradation of β-catenin to Suppress Metastasis of Hepatitis B-Associated Hepatocellular Carcinoma.
doi: 10.3389/fphar.2021.659325
Figure Lengend Snippet: FIGURE 8 | CP inhibited proliferation and migration of HBV-associated HCC ex vivo and in vivo. (A–C) HepG2-null and HepG2-HBx cells were grafted to the flank of nude mice by injecting the cells subcutaneously to observe tumor development, and volumes of tumors were determined at 3-day intervals. (D) Mice model with lung metastasis was established by tail vein injection of cancer cells. Upper panel, metastatic nodules in lungs. Lower panel, H&E staining of lung tissues. (E) Western blot analysis of tumor tissue showed that CP treatment decreased the expression levels of β-catenin and Cav-1, and enhanced the EMT process. (F) CP at concentrations from 60 to 120 μg/ml significantly decreased the primary as well as metastatic lesions in the zebrafish body when compared with control group. (G) H&E staining showed that CP suppressed the tumor growth in zebrafish. (H) Immunohistochemical staining of tumor in zebrafish showed CP treatment dramatically downregulated the expression levels of β-catenin, Cav-1 and Vimentin, and upregulated the expression level of E-cadherin. All values represented the means ± SD (n 6, *p < 0.05, **p < 0.01, ***p < 0.001).
Article Snippet: Primary antibodies used in our study contained Cav1(16447-1-AP, Proteintech, Chicago, United States), N-cadherin (22018-1-AP, Proteintech, Chicago, United States), E-cadherin (20874-1-AP, Proteintech, Chicago, United States),
Techniques: Migration, Ex Vivo, In Vivo, Injection, Staining, Western Blot, Expressing, Control, Immunohistochemical staining
Journal: Frontiers in Pharmacology
Article Title: Manpixiao Decoction Halted the Malignant Transformation of Precancerous Lesions of Gastric Cancer: From Network Prediction to In-Vivo Verification
doi: 10.3389/fphar.2022.927731
Figure Lengend Snippet: MPX downregulates the EGFR-PI3K-Akt related EMT pathway in PLGC model Rats. (A–B) Protein expression levels of EGFR, PI3K, p-AKT, AKT, N-cadherin, E-cadherin, Vimentin and β-catenin in gastric mucosa detected by western blot. Compared with control group, * p < 0.05, ** p < 0.01, *** p < 0.001; Compared with model group, # p < 0.05, ## p < 0.01.
Article Snippet: The primary antibodies including: Anti-EGFR antibody (#4267, CST), Anti-PI3 Kinase antibody (#67071-1-Ig, Proteintech), Anti-p-AKT antibody (#4060, CST), Anti-AKT antibody (#4691, CST), Anti-N-cadherin antibody (#14215S, CST), Anti-E-cadherin antibody (#60335-1-Ig, Proteintech) Anti-β-Catenin antibody (#610154, CST),
Techniques: Expressing, Western Blot, Control