Journal: bioRxiv

Article Title: Dorsal peduncular cortex activity modulates affective behaviors in mice

doi: 10.1101/2023.04.25.538301

Figure Lengend Snippet: A-B. Experimental timeline. C57BL/6J mice were injected with AAV5-hSyn-mCherry, AAV5-hSyn-hM3D-mCherry or AAV5-hSyn-hM4D-mCherry into the DP/DTT. Following 2 weeks, mice underwent a battery of behavioral tests comprised of the open field test (OFT), elevated plus maze (EPM), tail suspension test (TST), forced swim test (FST) and auditory fear conditioning (Fear) with a washout period of three days between each test. C. Representative images of an mCherry control virus-infused brain stained for mCherry (red) and the nuclear dye Hoechst (blue). D-F. Diagrams depicting overlaid viral spread for all mCherry ( D ), hM4D ( E ) and hM3D-injected ( F ) mice. Scale bar = 500µm.

Article Snippet: The fear conditioning apparatus was located within a sound-attenuated chamber (63.5cm wide, 36.8cm, high, 74.9cm deep; catalog #NIR-022MD, Med Associates).

Techniques: Injection, Battery, Suspension, Control, Virus, Staining

Journal: bioRxiv

Article Title: Dorsal peduncular cortex activity modulates affective behaviors in mice

doi: 10.1101/2023.04.25.538301

Figure Lengend Snippet: A.-B. Experimental timeline. Mice previously injected with hM3D, hM4D or control mCherry constructs in the DP/DTT were injected with the DREADD agonist C21 one hour prior to undergoing auditory fear conditioning. During acquisition, mice were placed in a sound-attenuated fear conditioning chamber and received 6 tone-shock pairings (0.5mA). Twenty-four hours later, auditory fear memory was evaluated in a different context in the absence of shocks. B. During fear acquisition, DP/DTT manipulations did not affect average tone freezing. C. hM4D mice displayed significantly more freezing than hM3D mice at tone presentations 4 and 6 during acquisition. D-E. DP/DTT manipulations did not affect average or individual post shock freezing episodes. F-I. During fear retrieval, DP/DTT manipulations did not affect freezing during average tone ( F ), individual tone ( G ), average post tone ( H ) or individual post tone ( I ) periods. indicates a significant difference between hM3D and hM4D groups ( p <0.05). Male (square) and female (circle) individual datapoints are displayed for transparency (see text for details).

Article Snippet: The fear conditioning apparatus was located within a sound-attenuated chamber (63.5cm wide, 36.8cm, high, 74.9cm deep; catalog #NIR-022MD, Med Associates).

Techniques: Injection, Control, Construct

Journal: bioRxiv

Article Title: Dopamine in the basal amygdala signals salient somatosensory events during fear learning

doi: 10.1101/716589

Figure Lengend Snippet: VTA neurons, and amongst them dopamine neurons, respond to footshocks and acquire CS - responsiveness. ( A ) Schematic showing an optrode with 16 recording channels implanted in the VTA of a DAT Cre × ChR2-eYFP mouse. ( B ) Post-hoc histological image showing the placement of an optrode (dashed line) in the VTA in the example mouse of panels B – H (FT7612). The tracks of two tetrodes are visible (arrows). Bottom, scheme of the design of four tetrodes (T1 - T4) around the optical fiber. ( C ) Illustration of optogenetic identification of putative DAT + units. Raster plot for four electrodes (E1-E4) of one tetrode, showing unsorted spikes aligned to the onsets of n = 100, 2 ms – long laser light pulses (blue shading). Spikes at 2-8 ms after the light pulse were collected and subjected to spike clustering (see Materials and Methods). ( D ) Time course of freezing of the example mouse during the three days of the fear learning protocol. ( E ) Spiking activity of a single opto-tagged putative DAT + unit during day 2 (training day). AP frequency (top) and z-score (bottom) are shown in response to the CS (averages over the n = 30 tone presentations for each tone block), as well as in response to footshocks (averages over the n = 6 footshock presentations; right). ( F ) Spiking activity of the same unit as (E), but for responses to CS presentations on day 3 (threat memory retrieval). Right, AP-waveforms for the unit shown in E, F. ( G, H ) Responses of all units in this mouse (FT7612) to tone presentations during day1 - day 3 (G), and to footshocks on day 2 (H). Z-scores were analyzed and plotted as a function of time (number of tone block). Units classified as CS - entrained are shown in pink, and the others in gray. Thick red and black lines show average ± S.E.M. across these groups, respectively. Square symbols connected by lines represent DAT + units identified by optotagging. ( I, J, K ) Venn-type diagrams showing the number of units in the different response classes and their overlap. The data in (I) is from the example mouse shown in panels B-H. Note the overall similar distribution and overlap of response types across n = 3 mice. Two US responsive units in mouse FT6963 showed a reduction in firing upon the footshock (J, dashed areas).

Article Snippet: A classical auditory cued threat memory paradigm was performed in a conditioning chamber of a NIR Video Fear Conditioning Optogenetics Package for Mouse (MED-VFC-OPTO-M, Med Associates Inc., VT, USA).

Techniques: Activity Assay, Blocking Assay