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micropipette  (Narishige inc)
96
Narishige inc micropipette
Figure 1. (A) One-cell rat embryos isolated from 0.7 dpc female SD rats. Note the presence of cumulus cell- free 1-cell embryos. Bar = 100 μm. (B) The i-GONAD procedure. Under appropriate anesthesia, ovary/oviduct/ uterus of a pregnant rat at 0.7 dpc was exposed under a dissecting microscope. After positioning the oviduct, it was gently grasped in forceps (a). A <t>micropipette</t> was then inserted into the lumen of the oviduct by piercing the oviduct wall and (controlled by a mouthpiece) approximately ~1.5 µL solution was immediately expelled into the oviduct (b). The injected substances can be easily identified by the co-injected Fast Green (c,d). After instillation, the entire oviduct was covered with a piece of wet Kimwipe towel and then subjected to in vivo electroporation using tweezer-type electrodes (e). After removal of electrodes, the electroporated area remains intact (f). (C) Detection of fluorescent 2-cell embryos after i-GONAD. When pregnant (0.7 dpc) SD rats were subjected to in vivo electroporation after intraoviductal instillation of Rhodamine + EGFP mRNA-containing solution (i-GONAD), some of the recovered 2-cell embryos exhibited distinct red and/or green fluorescence (a–c). In contrast, intraoviductal instillation of Rhodamine alone (without electroporation) (Control) resulted in embryos showing no fluorescence (d,e). Bar = 100 μm. (D) Flowchart of experiments to test the feasibility of i-GONAD for the production of mid-gestational fetuses carrying indels at a single target locus (Tyr).
Micropipette, supplied by Narishige inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/vertical+electric+puller/Narishige+PC-100+Vertical+puller/pm30104681-226-17-24
Average 96 stars, based on 1 article reviews
micropipette - by Bioz Stars, 2026-09
96/100 stars
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vertical electric puller  (Narishige inc)
96
Narishige inc vertical electric puller
Figure 1. (A) One-cell rat embryos isolated from 0.7 dpc female SD rats. Note the presence of cumulus cell- free 1-cell embryos. Bar = 100 μm. (B) The i-GONAD procedure. Under appropriate anesthesia, ovary/oviduct/ uterus of a pregnant rat at 0.7 dpc was exposed under a dissecting microscope. After positioning the oviduct, it was gently grasped in forceps (a). A <t>micropipette</t> was then inserted into the lumen of the oviduct by piercing the oviduct wall and (controlled by a mouthpiece) approximately ~1.5 µL solution was immediately expelled into the oviduct (b). The injected substances can be easily identified by the co-injected Fast Green (c,d). After instillation, the entire oviduct was covered with a piece of wet Kimwipe towel and then subjected to in vivo electroporation using tweezer-type electrodes (e). After removal of electrodes, the electroporated area remains intact (f). (C) Detection of fluorescent 2-cell embryos after i-GONAD. When pregnant (0.7 dpc) SD rats were subjected to in vivo electroporation after intraoviductal instillation of Rhodamine + EGFP mRNA-containing solution (i-GONAD), some of the recovered 2-cell embryos exhibited distinct red and/or green fluorescence (a–c). In contrast, intraoviductal instillation of Rhodamine alone (without electroporation) (Control) resulted in embryos showing no fluorescence (d,e). Bar = 100 μm. (D) Flowchart of experiments to test the feasibility of i-GONAD for the production of mid-gestational fetuses carrying indels at a single target locus (Tyr).
Vertical Electric Puller, supplied by Narishige inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/vertical+electric+puller/Narishige+PC-100+Vertical+puller/pm41793893-80-24-28
Average 96 stars, based on 1 article reviews
vertical electric puller - by Bioz Stars, 2026-09
96/100 stars
  Buy from Supplier

Image Search Results


Figure 1. (A) One-cell rat embryos isolated from 0.7 dpc female SD rats. Note the presence of cumulus cell- free 1-cell embryos. Bar = 100 μm. (B) The i-GONAD procedure. Under appropriate anesthesia, ovary/oviduct/ uterus of a pregnant rat at 0.7 dpc was exposed under a dissecting microscope. After positioning the oviduct, it was gently grasped in forceps (a). A micropipette was then inserted into the lumen of the oviduct by piercing the oviduct wall and (controlled by a mouthpiece) approximately ~1.5 µL solution was immediately expelled into the oviduct (b). The injected substances can be easily identified by the co-injected Fast Green (c,d). After instillation, the entire oviduct was covered with a piece of wet Kimwipe towel and then subjected to in vivo electroporation using tweezer-type electrodes (e). After removal of electrodes, the electroporated area remains intact (f). (C) Detection of fluorescent 2-cell embryos after i-GONAD. When pregnant (0.7 dpc) SD rats were subjected to in vivo electroporation after intraoviductal instillation of Rhodamine + EGFP mRNA-containing solution (i-GONAD), some of the recovered 2-cell embryos exhibited distinct red and/or green fluorescence (a–c). In contrast, intraoviductal instillation of Rhodamine alone (without electroporation) (Control) resulted in embryos showing no fluorescence (d,e). Bar = 100 μm. (D) Flowchart of experiments to test the feasibility of i-GONAD for the production of mid-gestational fetuses carrying indels at a single target locus (Tyr).

Journal: Scientific reports

Article Title: i-GONAD (improved genome-editing via oviductal nucleic acids delivery), a convenient in vivo tool to produce genome-edited rats.

doi: 10.1038/s41598-018-30137-x

Figure Lengend Snippet: Figure 1. (A) One-cell rat embryos isolated from 0.7 dpc female SD rats. Note the presence of cumulus cell- free 1-cell embryos. Bar = 100 μm. (B) The i-GONAD procedure. Under appropriate anesthesia, ovary/oviduct/ uterus of a pregnant rat at 0.7 dpc was exposed under a dissecting microscope. After positioning the oviduct, it was gently grasped in forceps (a). A micropipette was then inserted into the lumen of the oviduct by piercing the oviduct wall and (controlled by a mouthpiece) approximately ~1.5 µL solution was immediately expelled into the oviduct (b). The injected substances can be easily identified by the co-injected Fast Green (c,d). After instillation, the entire oviduct was covered with a piece of wet Kimwipe towel and then subjected to in vivo electroporation using tweezer-type electrodes (e). After removal of electrodes, the electroporated area remains intact (f). (C) Detection of fluorescent 2-cell embryos after i-GONAD. When pregnant (0.7 dpc) SD rats were subjected to in vivo electroporation after intraoviductal instillation of Rhodamine + EGFP mRNA-containing solution (i-GONAD), some of the recovered 2-cell embryos exhibited distinct red and/or green fluorescence (a–c). In contrast, intraoviductal instillation of Rhodamine alone (without electroporation) (Control) resulted in embryos showing no fluorescence (d,e). Bar = 100 μm. (D) Flowchart of experiments to test the feasibility of i-GONAD for the production of mid-gestational fetuses carrying indels at a single target locus (Tyr).

Article Snippet: Approximately 1.5 μL of solution was injected into the oviduct lumen upstream of the ampulla using a micropipette (prepared using an electric puller; PN-3; NARISHIGE, Tokyo, Japan) and an attached mouthpiece (Fig. 1B-a–d).

Techniques: Isolation, Microscopy, Injection, In Vivo, Electroporation, Fluorescence, Control