Journal: International Journal of Molecular Sciences

Article Title: Erk Inhibition as a Promising Therapeutic Strategy for High IL-8-Secreting and Low SPTAN1-Expressing Colorectal Cancer

doi: 10.3390/ijms25115658

Figure Lengend Snippet: FOLFOX and U0126 co-treatment significantly reduces long-term survival of reduced SPTAN1-expressing cells. Cells seeded at 1 × 10 3 per cell culture flask (surface area 25 cm 2 ) were incubated until adhesion and cultivated in medium supplemented with or without 10 μM of U0126 for 18 h. Then, cells were treated with FOLFOX supplemented with or without 10 μM of U0126 for 24 h, 48 h and 72 h. Subsequently, the medium was replaced and cells were cultivated in medium without supplements for a further seven days. Colonies were fixed with 4% paraformaldehyde and stained with 0.5% crystal violet. The number of colonies was quantified using the COLCOUNT TM system and Oxford Optronix ColCount software version 4.3.5.1. Columns show the mean number of colonies ± SD of reduced SPTAN1-expressing cells (shSPTAN1_3 blue columns) in comparison to the pLKO.1-transduced controls (pink columns). Co-treatment of U0126 and FOLFOX for 48 h as well as 72 h significantly inhibited the colony formation of all cell lines. p -values were calculated using one-way ANOVA; n = 3. The following p -values were considered as statistically significant: ns = not significant, * p < 0.05.

Article Snippet: The number of colonies was analyzed and quantified using the COLCOUNT TM system (Oxford Optronix Ltd., Abingdon, UK) and Oxford Optronix ColCount software version 4.3.5.1.

Techniques: Expressing, Cell Culture, Incubation, Staining, Software, Comparison