Journal: ACS nano

Article Title: Re-Engineered Pseudoviruses for Precise and Robust 3D Mapping of Viral Infection.

doi: 10.1021/acsnano.3c07767

Figure Lengend Snippet: Figure 1. Development of clickVSVs bearing the SARS-CoV-2 spike (S) Glycoprotein. (a) Schematic representation of the SARS- CoV-2 virus, the vesicular stomatitis virus (VSV), and the clickable pseudovirus (VSVΔG-eGFP-SARS-CoV-2-Sclick), generated using Genetic Code Expansion via amber suppression using orthogonal tRNA/tRNA-synthetase pairs (aaRS/tRNAaa). (b) Widefield fluorescence images of GFP (green) and Hoechst (white) of infected Vero E6 cells after 24 h of infection with VSVΔG-eGFP- SARS-CoV-2-S variants. (c) Influence of click-labeling on infectivity of clickVSVs: without (gray) or with prior click-labeling with Tetrazine-Cy5 (Tet-Cy5) (magenta). (d) Neutralization of wildtype VSVΔG-eGFP-SARS-CoV-2-S and clickable VSVΔG- eGFP-SARS-CoV-2-SCy5 with a neutralizing antibody (+nAB) or additional treatment with soluble ACE2 ectodomain (+sACE2).

Article Snippet: Vero E6 (CLS, 305008) and HEK293T (DSMZ, ACC635) cells were cultivated at 37 °C and 5% CO2 in T25-culture flasks (Thermo Scientific, #156340) with DMEM (Dulbeccos’s Modified Eagle’s Medium, Sigma-Aldrich, #D5796) supplemented with 10% FCS (Sigma-Aldrich, #F7524), 1% L-glutamine, and 1% Pen-Strep (Sigma-Aldrich, #P4333).

Techniques: Virus, Generated, Fluorescence, Infection, Labeling, Neutralization

Journal: Frontiers in Immunology

Article Title: Endothelial Immunity Trained by Coronavirus Infections, DAMP Stimulations and Regulated by Anti-Oxidant NRF2 May Contribute to Inflammations, Myelopoiesis, COVID-19 Cytokine Storms and Thromboembolism

doi: 10.3389/fimmu.2021.653110

Figure Lengend Snippet: (A–E) The mRNA transcripts of four types of coronavirus receptors such as membrane alanyl aminopeptidase (ANPEP, CD13, receptor for human coronavirus-229E), carcinoembryonic antigen family cell adhesion molecule 1 (CEACAM1, receptor for mouse hepatitis virus), angiotensin-converting enzyme 2 (ACE, receptor for SARS-CoV, SARS-CoV2), and dipeptidyl peptidase-4 (DPP4, receptor for MERS-CoV) as well as TMPRSS2 are found in two clusters of human heart endothelial cells. The data mining analyses were performed on the Single Cell RNA-Seq database of the Broad Institute of MIT and Harvard (Single CellBeta Portal; https://singlecell.broadinstitute.org/single_cell/study/SCP498/transcriptional-and-cellular-diversity-of-the-human-heart#study-summary ). (F–J) The mRNA transcripts of four types of coronavirus receptors such as ANPEP, CEACAM1, ACE, and DPP4 are found in mouse aortic endothelial cell clusters. The data mining analyses were performed on the Single Cell RNA-Seq database of the Broad Institute of MIT and Harvard (Single CellBeta Portal; https://singlecell.broadinstitute.org/single_cell/study/SCP289/single-cell-analysis-of-the-normal-mouse-aorta-reveals-functionally-distinct-endothelial-cell-populations#study-summay , PMID: 31146585). (F) ANPEP expressions in three endothelial cell clusters were circled in red in the Scatter; (G) ANPEP expressions in three endothelial cell clusters were boxed in red in the Distribution; (H) CEACAM1 expressions in three endothelial cell clusters were also boxed in read in the Distribution; (I) ACE expressions in three endothelial cell clusters were also boxed in read in the Distribution; (J) DPP4 expressions in three endothelial cell clusters were also boxed in read in the Distribution. (K) A new working model: Infection of vascular endothelial cells by SARS-CoV2/MERS-CoV causes innate immune responses in endothelial cells, which may induces cytokine storm, and triggers thromboembolism. The part of figure was created with BioRender.com .

Article Snippet: To test this hypothesis, we searched the expression of these four receptors and coronavirus S protein priming serine protease TMPRSS2 ( ) in human EC in the MIT-Harvard Broad Institute Single Cell Beta Porter single-cell RNA-Seq database, Study: Transcriptional and Cellular Diversity of the Human Heart ( https://singlecell.broadinstitute.org/single_cell/study/SCP498 ).

Techniques: Membrane, Virus, RNA Sequencing, Single-cell Analysis, Infection

Journal: Journal of Clinical Medicine

Article Title: Early Cellular and Humoral Responses Developed in Oncohematological Patients after Vaccination with One Dose against COVID-19

doi: 10.3390/jcm11102803

Figure Lengend Snippet: DCC and viral neutralization activity against SARS-CoV-2 infected cells of PBMCs of individuals with OHD before and after receiving one-dose vaccine. ( A ) The capacity of PBMCs from individuals with OHD to eliminate SARS-CoV-2-infected Vero E6 cells was determined by quantifying the production of Renilla (RLUs) after co-culture for 1 h. ( B ) DCC was assessed by measuring the activity of caspasa-3 in SARS-CoV-2-infected Vero E6 cells co-cultured with PBMCs from individuals with OHD, in comparison with healthy donors. Each dot in the graphs corresponds to mean ± SEM. Statistical significance between groups was calculated using one-way ANOVA test.

Article Snippet: Vero E6 (African green monkey kidney) cell line (ECACC 85020206) was kindly provided by Dr. Antonio Alcami (CBM Severo Ochoa, Madrid, Spain).

Techniques: Neutralization, Activity Assay, Infection, Co-Culture Assay, Cell Culture, Comparison

Journal: MedComm

Article Title: A potent neutralizing nanobody against SARS‐CoV‐2 with inhaled delivery potential

doi: 10.1002/mco2.60

Figure Lengend Snippet: Evaluation of neutralizing potential of seven Nbs using plaque reduction neutralization test. (A) Plaques formed in Vero E6 cells inoculated with 100 PFU SARS‐CoV‐2 and 10‐fold diluted Nbs. Cry1B Nb was used as the negative control. (B) Inhibitory rate of seven Nbs against authentic SARS‐CoV‐2 at the concentration of 50 and 5 μg/ml. (C) Plaques formed in Vero E6 cells inoculated with 100 PFU SARS‐CoV‐2 plus threefold diluted Nb16‐68 or Nb11‐59 mixture. (D) 50% ND 50 of Nb16‐68 or Nb11‐59 was determined. The results shown are mean value ± SD

Article Snippet: The HEK 293 and Vero E6 cells were obtained from the American Type Culture Collection (ATCC) and China Center for Type Culture Collection (CCTCC), respectively.

Techniques: Plaque Reduction Neutralization Test, Negative Control, Concentration Assay

Journal: BioMed Research International

Article Title: Animal Models for the Study of Rodent-Borne Hemorrhagic Fever Viruses: Arenaviruses and Hantaviruses

doi: 10.1155/2015/793257

Figure Lengend Snippet: Prototypical human pathogenic arenavirus strains and their origin.

Article Snippet: Chicava (Chi) , Bolivia (1993), fatal human isolate , 2 P Vero E6 , Guinea pigs, NHP , Pathogenesis , [ ] .

Techniques: Animal Model, Infection, Transfection, Derivative Assay, Recombinant, Virus

Journal: BioMed Research International

Article Title: Animal Models for the Study of Rodent-Borne Hemorrhagic Fever Viruses: Arenaviruses and Hantaviruses

doi: 10.1155/2015/793257

Figure Lengend Snippet: Prototypical hantavirus strains and their origin.

Article Snippet: Chicava (Chi) , Bolivia (1993), fatal human isolate , 2 P Vero E6 , Guinea pigs, NHP , Pathogenesis , [ ] .

Techniques: Animal Model, Infection