Journal: Biology

Article Title: Comparative Effects of Dexamethasone and ASC Secretome in an Ex Vivo Osteoarthritis Co-Culture Model

doi: 10.3390/biology15060493

Figure Lengend Snippet: ( a – c ) Gene expression of PTGS2 ( a ), IL-1B ( b ), and VEGFA ( c ) in cartilage explants (CEs) after the treatments. Data are expressed as 2 −ΔΔCt (TBP was used as a housekeeping gene) ( d ) COX2 expression in CEs after the treatments and a representative immunoblot (below). Data were normalized to GAPDH expression and expressed as relative values (CTR = 1). ( e – g ) Gene expression of PTGS2 ( e ), IL-1β ( f ), and VEGFA ( g ) in synovial membrane explants after the treatments. Data are expressed as 2 −ΔΔCt (TBP was used as a housekeeping gene) ( h , i ) COX2 ( h ) and IDO ( i ) expression in synovial membrane explants after the treatments and representative immunoblots (below). Data were normalized to GAPDH expression and expressed as relative values (CTR = 1). ( j ) Nitric oxide (NO) release (µM) in supernatants. Data are expressed as mean ± SEM of n = 4–5 independent experiments. Significance versus CTR is shown as * p < 0.05, ** p < 0.01, and *** p < 0.001.

Article Snippet: Subsequently, the expression levels of different genes were quantified through reverse transcription quantitative polymerase chain reaction (RT-qPCR) employing TaqMan technology using the following probes: PTGS2 (hs00153133_m1), MMP3 (hs00968305_m1), MMP13 (hs00 233992_m1), SOX9 (hs00165814_m1), COL2A1 (hs01060345_m1), IL-1β (Hs01555410_m1), TIMP1 (Hs99999139_m1), ACAN (hs00153936_m1), COL10A1 (hs00166657_m1), RUNX2 (hs00231692_m1), VEGFA (hs00900055_m1), FN1 (Hs01549976_m1), CTGF (Hs00170014_m1), and TBP (Hs00427620_m1) (Thermo Fisher Scientific, Waltham, MA, USA).

Techniques: Gene Expression, Expressing, Western Blot, Membrane

Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Article Title: Deficiency of HtrA4 in BeWo cells downregulates angiogenesis through IL-6/JAK/STAT3 signaling.

doi: 10.1016/j.biopha.2023.115288

Figure Lengend Snippet: Fig. 3. The levels of secreted IL-6 and VEGFA. The levels of secreted IL-6 and VEGFA were measured by ELISA in BeWo WT, BeWo KO, and BeWo KO-HtrA4 rescue cells co-cultured with HUVECs, as well as in only HUVECs as a control. (A) The levels of secreted IL-6 were higher in the supernatant of HUVECs co- cultured with BeWo WT or BeWo KO-HtrA4 rescue compared to co-culture with BeWo KO or only HUVECs (n = 3 * p < 0.05, ** p < 0.01, and *** p < 0.001). (B) The levels of secreted VEGFA were the highest in the su pernatant of HUVECs co-cultured with BeWo WT cells and the lowest in those co-cultured with BeWo KO cells (n = 3 * p < 0.05, ** p < 0.01, and *** p < 0.001).

Article Snippet: The concentration of VEGFA was measured in the supernatant of the co-cultured HUVECs or only HUVECs, using a VEGFA kit (Cusabio Technology LLC.

Techniques: Enzyme-linked Immunosorbent Assay, Cell Culture, Control, Co-Culture Assay

Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Article Title: Deficiency of HtrA4 in BeWo cells downregulates angiogenesis through IL-6/JAK/STAT3 signaling.

doi: 10.1016/j.biopha.2023.115288

Figure Lengend Snippet: Fig. 4. JAK/STAT3 signaling pathway in HUVECs. (A) The HUVECs were co-cultured with BeWo WT, BeWo KO, and BeWo KO-HtrA4 rescue cells, and only HUVECs were cultured as a control. The expression levels of STAT3, p-STAT3, JAK2, and p-JAK2 were examined by western blot analysis, while the expression level of VEGFA was examined by RT-PCR. The results were quantified and presented as bar graphs (n = 3 * p < 0.05, ** p < 0.01, and *** p < 0.001).

Article Snippet: The concentration of VEGFA was measured in the supernatant of the co-cultured HUVECs or only HUVECs, using a VEGFA kit (Cusabio Technology LLC.

Techniques: Cell Culture, Control, Expressing, Western Blot, Reverse Transcription Polymerase Chain Reaction

Journal: BMC pregnancy and childbirth

Article Title: Expression of insulin-like growth factor binding protein-3 in HELLP syndrome.

doi: 10.1186/s12884-023-06074-7

Figure Lengend Snippet: Fig. 1 Levels of IGFBP-3/IGF-1/TGF-β1/VEGF in maternal blood of three groups (*P < 0.05, **P < 0.01)

Article Snippet: The reagents comprised: Human IGFBP-3 ELISA kit (R&B, DY675, USA), IGF-1 ELISA kit (R&B, DY291-05, USA), TGF-β1 ELISA kit (R&B, DY240-5,USA), Human VEGF ELISA Kit (R&B, DY293B-5,USA), goat serum (Proteintech Inc., B900780, USA), Tween-20 (Sigma Inc., T2700, USA), substrate solution (R&B,DY999, USA), bovine serum albumin (Sigma, B2064-50,USA), Annexin V-FITC/PI apoptosis assay kit (Novizan, A21101, China), endothelial cell culture medium (ScienCell, 1001, USA), recombinant human IGFBP-3 protein (YiqiaoShenzhou Technology Co., Ltd., 10430-H07H, China).

Techniques:

Journal: BMC pregnancy and childbirth

Article Title: Expression of insulin-like growth factor binding protein-3 in HELLP syndrome.

doi: 10.1186/s12884-023-06074-7

Figure Lengend Snippet: Fig. 2 Levels of IGFBP-3/IGF-1/TGF-β1/VEGF in umbilical blood of three groups (*P < 0.05, **P < 0.01)

Article Snippet: The reagents comprised: Human IGFBP-3 ELISA kit (R&B, DY675, USA), IGF-1 ELISA kit (R&B, DY291-05, USA), TGF-β1 ELISA kit (R&B, DY240-5,USA), Human VEGF ELISA Kit (R&B, DY293B-5,USA), goat serum (Proteintech Inc., B900780, USA), Tween-20 (Sigma Inc., T2700, USA), substrate solution (R&B,DY999, USA), bovine serum albumin (Sigma, B2064-50,USA), Annexin V-FITC/PI apoptosis assay kit (Novizan, A21101, China), endothelial cell culture medium (ScienCell, 1001, USA), recombinant human IGFBP-3 protein (YiqiaoShenzhou Technology Co., Ltd., 10430-H07H, China).

Techniques:

Journal: BMC pregnancy and childbirth

Article Title: Expression of insulin-like growth factor binding protein-3 in HELLP syndrome.

doi: 10.1186/s12884-023-06074-7

Figure Lengend Snippet: Fig. 3 Correlation analysis between IGFBP-3 and IGF-1/TGF-β1/VEGF (**P < 0.01, Line 1: results of correlation analysis in maternal blood; Line 2: results of correlation analysis in umbilical blood. The correlation coefficient (r) and regression equation are shown in the figure

Article Snippet: The reagents comprised: Human IGFBP-3 ELISA kit (R&B, DY675, USA), IGF-1 ELISA kit (R&B, DY291-05, USA), TGF-β1 ELISA kit (R&B, DY240-5,USA), Human VEGF ELISA Kit (R&B, DY293B-5,USA), goat serum (Proteintech Inc., B900780, USA), Tween-20 (Sigma Inc., T2700, USA), substrate solution (R&B,DY999, USA), bovine serum albumin (Sigma, B2064-50,USA), Annexin V-FITC/PI apoptosis assay kit (Novizan, A21101, China), endothelial cell culture medium (ScienCell, 1001, USA), recombinant human IGFBP-3 protein (YiqiaoShenzhou Technology Co., Ltd., 10430-H07H, China).

Techniques: