vector phm1 Search Results


amino acids 3046 3447  (ATCC)
93
ATCC amino acids 3046 3447
Amino Acids 3046 3447, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cpet expression vectors cyanophage p-hm1_cpet (phm1_028)  (Eurofins)
90
Eurofins cpet expression vectors cyanophage p-hm1_cpet (phm1_028)
Cpet Expression Vectors Cyanophage P Hm1 Cpet (Phm1 028), supplied by Eurofins, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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vector phm1  (New England Biolabs)
86
New England Biolabs vector phm1
(A) Symptoms caused by Xoo strains PXO99 A (wild type) (1), <t>pHM1</t> (2), 2C10 (clone of RS105 genomic library) (3), 8E07 (clone of RS105 genomic library) (4), pHMavrRxo1 RS105 (5) and MgCl 2 (10 mM) (6) in Nicotiana benthamiana . Xoo strains [1×10 8 colony-forming units (cfu)/mL] were inoculated to N. benthamiana (4–6 weeks old) with a needleless syringe, and symptoms were measured at 2 days post-inoculation (dpi). (B) The phenotype of interactions between maize lines B73 and five AvrRxo1 clones: pHMavrRxo1 RS105 , pHMavrRxo1 RS85 , pHMavrRxo1 SDAU-1 , pHMavrRxo1 JSB2-24 and pHMavrRxo1 HNB8-47 . Xoo strains [1×10 8 cfu/mL] were infiltrated into B73 (4 weeks old) with a needleless syringe, and symptoms were measured at 2 dpi. Infiltration of B73 with PXO99 A containing avrRxo1 gene results in a hypersensitive response at 2 dpi. PXO99 A and PXO99 A (pHM1) produced no reaction. (C) Symptoms caused by five AvrRxo1 clones in Xoo strains PXO99 A (1) pHM1, (2) pHMavrRxo1 RS105 (3), pHMavrRxo1 RS85 (4), pHMavrRxo1 SDAU-1 (5), pHMavrRxo1 JSB2-24 (6), pHMavrRxo1 HNB8-47 (7), and PXO61 (8) in N. benthamiana . All experiments were repeated three times with similar results.
Vector Phm1, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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(A) Symptoms caused by Xoo strains PXO99 A (wild type) (1), pHM1 (2), 2C10 (clone of RS105 genomic library) (3), 8E07 (clone of RS105 genomic library) (4), pHMavrRxo1 RS105 (5) and MgCl 2 (10 mM) (6) in Nicotiana benthamiana . Xoo strains [1×10 8 colony-forming units (cfu)/mL] were inoculated to N. benthamiana (4–6 weeks old) with a needleless syringe, and symptoms were measured at 2 days post-inoculation (dpi). (B) The phenotype of interactions between maize lines B73 and five AvrRxo1 clones: pHMavrRxo1 RS105 , pHMavrRxo1 RS85 , pHMavrRxo1 SDAU-1 , pHMavrRxo1 JSB2-24 and pHMavrRxo1 HNB8-47 . Xoo strains [1×10 8 cfu/mL] were infiltrated into B73 (4 weeks old) with a needleless syringe, and symptoms were measured at 2 dpi. Infiltration of B73 with PXO99 A containing avrRxo1 gene results in a hypersensitive response at 2 dpi. PXO99 A and PXO99 A (pHM1) produced no reaction. (C) Symptoms caused by five AvrRxo1 clones in Xoo strains PXO99 A (1) pHM1, (2) pHMavrRxo1 RS105 (3), pHMavrRxo1 RS85 (4), pHMavrRxo1 SDAU-1 (5), pHMavrRxo1 JSB2-24 (6), pHMavrRxo1 HNB8-47 (7), and PXO61 (8) in N. benthamiana . All experiments were repeated three times with similar results.

Journal: PLoS ONE

Article Title: Domain Dissection of AvrRxo1 for Suppressor, Avirulence and Cytotoxicity Functions

doi: 10.1371/journal.pone.0113875

Figure Lengend Snippet: (A) Symptoms caused by Xoo strains PXO99 A (wild type) (1), pHM1 (2), 2C10 (clone of RS105 genomic library) (3), 8E07 (clone of RS105 genomic library) (4), pHMavrRxo1 RS105 (5) and MgCl 2 (10 mM) (6) in Nicotiana benthamiana . Xoo strains [1×10 8 colony-forming units (cfu)/mL] were inoculated to N. benthamiana (4–6 weeks old) with a needleless syringe, and symptoms were measured at 2 days post-inoculation (dpi). (B) The phenotype of interactions between maize lines B73 and five AvrRxo1 clones: pHMavrRxo1 RS105 , pHMavrRxo1 RS85 , pHMavrRxo1 SDAU-1 , pHMavrRxo1 JSB2-24 and pHMavrRxo1 HNB8-47 . Xoo strains [1×10 8 cfu/mL] were infiltrated into B73 (4 weeks old) with a needleless syringe, and symptoms were measured at 2 dpi. Infiltration of B73 with PXO99 A containing avrRxo1 gene results in a hypersensitive response at 2 dpi. PXO99 A and PXO99 A (pHM1) produced no reaction. (C) Symptoms caused by five AvrRxo1 clones in Xoo strains PXO99 A (1) pHM1, (2) pHMavrRxo1 RS105 (3), pHMavrRxo1 RS85 (4), pHMavrRxo1 SDAU-1 (5), pHMavrRxo1 JSB2-24 (6), pHMavrRxo1 HNB8-47 (7), and PXO61 (8) in N. benthamiana . All experiments were repeated three times with similar results.

Article Snippet: The vector pHM1 was digested to completion with Bam HI (NEB, USA) and treated with calf intestinal alkaline phosphatase (NEB, USA).

Techniques: Clone Assay, Produced

(A) The phenotype of interactions between maize lines B73 and deletion mutants of AvrRxo1. Infiltration of B73 with PXO99 A containing avrRxo1 gene results in HR at 2 dpi. PXO99 A (pHM1) as negative control. Fragments AvrRxo1(17–421), AvrRxo1(52–421), AvrRxo1(109–421), AvrRxo1(140–421), AvrRxo1(186–421), AvrRxo1(1–159), AvrRxo1(1–193), AvrRxo1(1–278), AvrRxo1(1–373), AvrRxo1(1–412), AvrRxo1(1–417), and AvrRxo1(1–418), abolished induce HR on B73, while fragments AvrRxo1(1–419) and AvrRxo1(1–420) produce HR on B73 at 2 dpi. (B) Suppressions of non-host HR by deletion mutants of AvrRxo1 in N. benthamiana . Infiltration of N. benthamiana with PXO99 A (pHM:avrRxo1) (1) and PXO99 A (pHM1) (2) as control. Fragments AvrRxo1(17–421) (3), AvrRxo1(52–421) (4), AvrRxo1(109–421) (5), AvrRxo1(140–421) (6), AvrRxo1(186–421) (7), AvrRxo1(1–412) (8), AvrRxo1(1–417) (9), AvrRxo1(1–418) (10), AvrRxo1(1–419) (11), AvrRxo1(1–420) (12) did not suppress the non-host HR caused by PXO99 A in N. benthamiana . RT-PCR was used to confirm the expression of avrRxo1 in inoculated leaves. All experiments were repeated three times with similar results.

Journal: PLoS ONE

Article Title: Domain Dissection of AvrRxo1 for Suppressor, Avirulence and Cytotoxicity Functions

doi: 10.1371/journal.pone.0113875

Figure Lengend Snippet: (A) The phenotype of interactions between maize lines B73 and deletion mutants of AvrRxo1. Infiltration of B73 with PXO99 A containing avrRxo1 gene results in HR at 2 dpi. PXO99 A (pHM1) as negative control. Fragments AvrRxo1(17–421), AvrRxo1(52–421), AvrRxo1(109–421), AvrRxo1(140–421), AvrRxo1(186–421), AvrRxo1(1–159), AvrRxo1(1–193), AvrRxo1(1–278), AvrRxo1(1–373), AvrRxo1(1–412), AvrRxo1(1–417), and AvrRxo1(1–418), abolished induce HR on B73, while fragments AvrRxo1(1–419) and AvrRxo1(1–420) produce HR on B73 at 2 dpi. (B) Suppressions of non-host HR by deletion mutants of AvrRxo1 in N. benthamiana . Infiltration of N. benthamiana with PXO99 A (pHM:avrRxo1) (1) and PXO99 A (pHM1) (2) as control. Fragments AvrRxo1(17–421) (3), AvrRxo1(52–421) (4), AvrRxo1(109–421) (5), AvrRxo1(140–421) (6), AvrRxo1(186–421) (7), AvrRxo1(1–412) (8), AvrRxo1(1–417) (9), AvrRxo1(1–418) (10), AvrRxo1(1–419) (11), AvrRxo1(1–420) (12) did not suppress the non-host HR caused by PXO99 A in N. benthamiana . RT-PCR was used to confirm the expression of avrRxo1 in inoculated leaves. All experiments were repeated three times with similar results.

Article Snippet: The vector pHM1 was digested to completion with Bam HI (NEB, USA) and treated with calf intestinal alkaline phosphatase (NEB, USA).

Techniques: Negative Control, Reverse Transcription Polymerase Chain Reaction, Expressing

(A) The phenotype of interactions between maize lines B73 and four AvrRxo1 mutants: AvrRxo1(H71A), AvrRxo1(G165A), AvrRxo1(K166N), and AvrRxo1(nls). Mutants AvrRxo1(H71A) and AvrRxo1(G165A) induce HR at 2 dpi, however mutants AvrRxo1(K166N) and AvrRxo1(nls) are abolsihed to elicit HR on B73. (B) The phenotype of suppression of non-host HR in N. benthamiana caused by PXO99 A . PXO99 A (pHM1) was used as negative control and PXO99 A (pHM:avrRxo1) as positive control. PXO99 A containing AvrRxo1(H71A) (6) did not elicit the non-host HR in N. benthamiana , while PXO99 A containing the fragments AvrRxo1(G165A) (3), AvrRxo1(K166N) (4), and nls (5) can still elicit non-host HR. RT-PCR was used to confirm the expression of avrRxo1 in inoculated leaves. All experiments were repeated three times with similar results.

Journal: PLoS ONE

Article Title: Domain Dissection of AvrRxo1 for Suppressor, Avirulence and Cytotoxicity Functions

doi: 10.1371/journal.pone.0113875

Figure Lengend Snippet: (A) The phenotype of interactions between maize lines B73 and four AvrRxo1 mutants: AvrRxo1(H71A), AvrRxo1(G165A), AvrRxo1(K166N), and AvrRxo1(nls). Mutants AvrRxo1(H71A) and AvrRxo1(G165A) induce HR at 2 dpi, however mutants AvrRxo1(K166N) and AvrRxo1(nls) are abolsihed to elicit HR on B73. (B) The phenotype of suppression of non-host HR in N. benthamiana caused by PXO99 A . PXO99 A (pHM1) was used as negative control and PXO99 A (pHM:avrRxo1) as positive control. PXO99 A containing AvrRxo1(H71A) (6) did not elicit the non-host HR in N. benthamiana , while PXO99 A containing the fragments AvrRxo1(G165A) (3), AvrRxo1(K166N) (4), and nls (5) can still elicit non-host HR. RT-PCR was used to confirm the expression of avrRxo1 in inoculated leaves. All experiments were repeated three times with similar results.

Article Snippet: The vector pHM1 was digested to completion with Bam HI (NEB, USA) and treated with calf intestinal alkaline phosphatase (NEB, USA).

Techniques: Negative Control, Positive Control, Reverse Transcription Polymerase Chain Reaction, Expressing

Strains and plasmids used in this study.

Journal: PLoS ONE

Article Title: Domain Dissection of AvrRxo1 for Suppressor, Avirulence and Cytotoxicity Functions

doi: 10.1371/journal.pone.0113875

Figure Lengend Snippet: Strains and plasmids used in this study.

Article Snippet: The vector pHM1 was digested to completion with Bam HI (NEB, USA) and treated with calf intestinal alkaline phosphatase (NEB, USA).

Techniques: Isolation, Plasmid Preparation, Expressing