vector control Search Results


rabbit immunoglobulin  (Vector Laboratories)
96
Vector Laboratories rabbit immunoglobulin
Rabbit Immunoglobulin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit immunoglobulin/product/Vector Laboratories
Average 96 stars, based on 1 article reviews
rabbit immunoglobulin - by Bioz Stars, 2026-06
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control gfp  (Sino Biological)
95
Sino Biological control gfp
Control Gfp, supplied by Sino Biological, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 95 stars, based on 1 article reviews
control gfp - by Bioz Stars, 2026-06
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negative control nc shrna  (OriGene)
95
OriGene negative control nc shrna
Figure 5. Interference with <t>endogenous</t> <t>HMGB1</t> expression increases the sensitivity of HeLa cells to cisplatin. (A) HMGB1 protein expression levels were detected by western blotting in cytoplasmic and nuclear extracts from HeLa cells transfected with HMGB1 <t>shRNA</t> or NC shRNA, and incubated with/without 10 µg/ml cisplatin for 24 h. β‑Tubulin was used as a cytoplasmic marker and lamin B1 as a nuclear marker. (B) Growth inhibition rates were determined by MTT assay in HeLa and HeLa/DDP cells transfected with HMGB1 shRNA or NC shRNA, and incubated with 10 µg/ml cisplatin for 24, 48 and 72 h. **P<0.01, with comparisons indicated by brackets. HMGB1, high mobility group box 1 protein; ctrl, control; shRNA, short hairpin RNA; NC, negative control.
Negative Control Nc Shrna, supplied by OriGene, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/negative control nc shrna/product/OriGene
Average 95 stars, based on 1 article reviews
negative control nc shrna - by Bioz Stars, 2026-06
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svec  (OriGene)
93
OriGene svec
Figure 5. Interference with <t>endogenous</t> <t>HMGB1</t> expression increases the sensitivity of HeLa cells to cisplatin. (A) HMGB1 protein expression levels were detected by western blotting in cytoplasmic and nuclear extracts from HeLa cells transfected with HMGB1 <t>shRNA</t> or NC shRNA, and incubated with/without 10 µg/ml cisplatin for 24 h. β‑Tubulin was used as a cytoplasmic marker and lamin B1 as a nuclear marker. (B) Growth inhibition rates were determined by MTT assay in HeLa and HeLa/DDP cells transfected with HMGB1 shRNA or NC shRNA, and incubated with 10 µg/ml cisplatin for 24, 48 and 72 h. **P<0.01, with comparisons indicated by brackets. HMGB1, high mobility group box 1 protein; ctrl, control; shRNA, short hairpin RNA; NC, negative control.
Svec, supplied by OriGene, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/svec/product/OriGene
Average 93 stars, based on 1 article reviews
svec - by Bioz Stars, 2026-06
93/100 stars
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control vector  (OriGene)
93
OriGene control vector
Figure 5. Interference with <t>endogenous</t> <t>HMGB1</t> expression increases the sensitivity of HeLa cells to cisplatin. (A) HMGB1 protein expression levels were detected by western blotting in cytoplasmic and nuclear extracts from HeLa cells transfected with HMGB1 <t>shRNA</t> or NC shRNA, and incubated with/without 10 µg/ml cisplatin for 24 h. β‑Tubulin was used as a cytoplasmic marker and lamin B1 as a nuclear marker. (B) Growth inhibition rates were determined by MTT assay in HeLa and HeLa/DDP cells transfected with HMGB1 shRNA or NC shRNA, and incubated with 10 µg/ml cisplatin for 24, 48 and 72 h. **P<0.01, with comparisons indicated by brackets. HMGB1, high mobility group box 1 protein; ctrl, control; shRNA, short hairpin RNA; NC, negative control.
Control Vector, supplied by OriGene, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/control vector/product/OriGene
Average 93 stars, based on 1 article reviews
control vector - by Bioz Stars, 2026-06
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vector tr30033  (OriGene)
93
OriGene vector tr30033
Figure 5. Interference with <t>endogenous</t> <t>HMGB1</t> expression increases the sensitivity of HeLa cells to cisplatin. (A) HMGB1 protein expression levels were detected by western blotting in cytoplasmic and nuclear extracts from HeLa cells transfected with HMGB1 <t>shRNA</t> or NC shRNA, and incubated with/without 10 µg/ml cisplatin for 24 h. β‑Tubulin was used as a cytoplasmic marker and lamin B1 as a nuclear marker. (B) Growth inhibition rates were determined by MTT assay in HeLa and HeLa/DDP cells transfected with HMGB1 shRNA or NC shRNA, and incubated with 10 µg/ml cisplatin for 24, 48 and 72 h. **P<0.01, with comparisons indicated by brackets. HMGB1, high mobility group box 1 protein; ctrl, control; shRNA, short hairpin RNA; NC, negative control.
Vector Tr30033, supplied by OriGene, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/vector tr30033/product/OriGene
Average 93 stars, based on 1 article reviews
vector tr30033 - by Bioz Stars, 2026-06
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secondary antibodies  (Vector Laboratories)
96
Vector Laboratories secondary antibodies
Figure 5. Interference with <t>endogenous</t> <t>HMGB1</t> expression increases the sensitivity of HeLa cells to cisplatin. (A) HMGB1 protein expression levels were detected by western blotting in cytoplasmic and nuclear extracts from HeLa cells transfected with HMGB1 <t>shRNA</t> or NC shRNA, and incubated with/without 10 µg/ml cisplatin for 24 h. β‑Tubulin was used as a cytoplasmic marker and lamin B1 as a nuclear marker. (B) Growth inhibition rates were determined by MTT assay in HeLa and HeLa/DDP cells transfected with HMGB1 shRNA or NC shRNA, and incubated with 10 µg/ml cisplatin for 24, 48 and 72 h. **P<0.01, with comparisons indicated by brackets. HMGB1, high mobility group box 1 protein; ctrl, control; shRNA, short hairpin RNA; NC, negative control.
Secondary Antibodies, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/secondary antibodies/product/Vector Laboratories
Average 96 stars, based on 1 article reviews
secondary antibodies - by Bioz Stars, 2026-06
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anti mouse igg magenta  (Vector Laboratories)
96
Vector Laboratories anti mouse igg magenta
Figure 5. Interference with <t>endogenous</t> <t>HMGB1</t> expression increases the sensitivity of HeLa cells to cisplatin. (A) HMGB1 protein expression levels were detected by western blotting in cytoplasmic and nuclear extracts from HeLa cells transfected with HMGB1 <t>shRNA</t> or NC shRNA, and incubated with/without 10 µg/ml cisplatin for 24 h. β‑Tubulin was used as a cytoplasmic marker and lamin B1 as a nuclear marker. (B) Growth inhibition rates were determined by MTT assay in HeLa and HeLa/DDP cells transfected with HMGB1 shRNA or NC shRNA, and incubated with 10 µg/ml cisplatin for 24, 48 and 72 h. **P<0.01, with comparisons indicated by brackets. HMGB1, high mobility group box 1 protein; ctrl, control; shRNA, short hairpin RNA; NC, negative control.
Anti Mouse Igg Magenta, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
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scrambled negative control non effective shrna  (OriGene)
96
OriGene scrambled negative control non effective shrna
KEY RESOURCES TABLE
Scrambled Negative Control Non Effective Shrna, supplied by OriGene, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
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non targeting scramble control  (OriGene)
95
OriGene non targeting scramble control
KEY RESOURCES TABLE
Non Targeting Scramble Control, supplied by OriGene, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 95 stars, based on 1 article reviews
non targeting scramble control - by Bioz Stars, 2026-06
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expression plasmid pcmv3 msnx10 ha  (Sino Biological)
93
Sino Biological expression plasmid pcmv3 msnx10 ha
KEY RESOURCES TABLE
Expression Plasmid Pcmv3 Msnx10 Ha, supplied by Sino Biological, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti rat igg polymer  (Vector Laboratories)
94
Vector Laboratories anti rat igg polymer
KEY RESOURCES TABLE
Anti Rat Igg Polymer, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Figure 5. Interference with endogenous HMGB1 expression increases the sensitivity of HeLa cells to cisplatin. (A) HMGB1 protein expression levels were detected by western blotting in cytoplasmic and nuclear extracts from HeLa cells transfected with HMGB1 shRNA or NC shRNA, and incubated with/without 10 µg/ml cisplatin for 24 h. β‑Tubulin was used as a cytoplasmic marker and lamin B1 as a nuclear marker. (B) Growth inhibition rates were determined by MTT assay in HeLa and HeLa/DDP cells transfected with HMGB1 shRNA or NC shRNA, and incubated with 10 µg/ml cisplatin for 24, 48 and 72 h. **P<0.01, with comparisons indicated by brackets. HMGB1, high mobility group box 1 protein; ctrl, control; shRNA, short hairpin RNA; NC, negative control.

Journal: Molecular medicine reports

Article Title: Inhibiting the cytoplasmic location of HMGB1 reverses cisplatin resistance in human cervical cancer cells.

doi: 10.3892/mmr.2016.6003

Figure Lengend Snippet: Figure 5. Interference with endogenous HMGB1 expression increases the sensitivity of HeLa cells to cisplatin. (A) HMGB1 protein expression levels were detected by western blotting in cytoplasmic and nuclear extracts from HeLa cells transfected with HMGB1 shRNA or NC shRNA, and incubated with/without 10 µg/ml cisplatin for 24 h. β‑Tubulin was used as a cytoplasmic marker and lamin B1 as a nuclear marker. (B) Growth inhibition rates were determined by MTT assay in HeLa and HeLa/DDP cells transfected with HMGB1 shRNA or NC shRNA, and incubated with 10 µg/ml cisplatin for 24, 48 and 72 h. **P<0.01, with comparisons indicated by brackets. HMGB1, high mobility group box 1 protein; ctrl, control; shRNA, short hairpin RNA; NC, negative control.

Article Snippet: The human HMGB1 short hairpin (sh) RNAs (cat. no. TG316576) and negative control (NC) shRNA (cat. no. TR30013) were obtained from OriGene Technologies, Inc. (Rockville, MD, USA).

Techniques: Expressing, Western Blot, Transfection, shRNA, Incubation, Marker, Inhibition, MTT Assay, Control, Negative Control

Figure 6. Transfection with HMGB1 induces cell apoptosis in HeLa cells. HeLa cells were transfected with HMGB1 shRNA or NC shRNA and treated with 10 µg/ml of cisplatin for 48 h. (A) Cell apoptosis was detected by an Annexin V‑PI dual staining analysis, **P<0.01 (B). Expression levels of apoptosis‑related proteins were detected by western blot analysis of whole cell lysates. β‑actin was used as an internal control (C). HMGB1, high mobility group box protein 1; shRNA, short hairpin RNA; NC, negative control; ctrl, control; PI, propidium iodide; PARP, poly ADP ribose polymerase.

Journal: Molecular medicine reports

Article Title: Inhibiting the cytoplasmic location of HMGB1 reverses cisplatin resistance in human cervical cancer cells.

doi: 10.3892/mmr.2016.6003

Figure Lengend Snippet: Figure 6. Transfection with HMGB1 induces cell apoptosis in HeLa cells. HeLa cells were transfected with HMGB1 shRNA or NC shRNA and treated with 10 µg/ml of cisplatin for 48 h. (A) Cell apoptosis was detected by an Annexin V‑PI dual staining analysis, **P<0.01 (B). Expression levels of apoptosis‑related proteins were detected by western blot analysis of whole cell lysates. β‑actin was used as an internal control (C). HMGB1, high mobility group box protein 1; shRNA, short hairpin RNA; NC, negative control; ctrl, control; PI, propidium iodide; PARP, poly ADP ribose polymerase.

Article Snippet: The human HMGB1 short hairpin (sh) RNAs (cat. no. TG316576) and negative control (NC) shRNA (cat. no. TR30013) were obtained from OriGene Technologies, Inc. (Rockville, MD, USA).

Techniques: Transfection, shRNA, Staining, Expressing, Western Blot, Control, Negative Control

Figure 7. Cell autophagy induced by HMGB1 treatment in HeLa cells is mediated by the ERK1/2 pathway. Western blot analysis of LC3‑I, LC3‑II, p62, ERK1/2 and p‑ERK1/2 protein levels was performed in (A) 24 h serum‑starved HeLa cells, treated with 100 µg/ml rhHMGB1 or 100 µg/ml BSA for 48 h, and (B) HeLa cells transfected with NC shRNA and HMGB1 shRNA. β‑actin was used as a loading control. HMGB1, high mobility group box protein 1; ERK1/2, extracellular signal‑related kinases 1 and 2; LC3, microtubule associated protein 1 light chain 3; shRNA, short hairpin RNA; NC, negative control; p62, nucleoporin p62; p, phosphorylated; BSA, bovine serum albumin; rh, recombinant human.

Journal: Molecular medicine reports

Article Title: Inhibiting the cytoplasmic location of HMGB1 reverses cisplatin resistance in human cervical cancer cells.

doi: 10.3892/mmr.2016.6003

Figure Lengend Snippet: Figure 7. Cell autophagy induced by HMGB1 treatment in HeLa cells is mediated by the ERK1/2 pathway. Western blot analysis of LC3‑I, LC3‑II, p62, ERK1/2 and p‑ERK1/2 protein levels was performed in (A) 24 h serum‑starved HeLa cells, treated with 100 µg/ml rhHMGB1 or 100 µg/ml BSA for 48 h, and (B) HeLa cells transfected with NC shRNA and HMGB1 shRNA. β‑actin was used as a loading control. HMGB1, high mobility group box protein 1; ERK1/2, extracellular signal‑related kinases 1 and 2; LC3, microtubule associated protein 1 light chain 3; shRNA, short hairpin RNA; NC, negative control; p62, nucleoporin p62; p, phosphorylated; BSA, bovine serum albumin; rh, recombinant human.

Article Snippet: The human HMGB1 short hairpin (sh) RNAs (cat. no. TG316576) and negative control (NC) shRNA (cat. no. TR30013) were obtained from OriGene Technologies, Inc. (Rockville, MD, USA).

Techniques: Western Blot, Transfection, shRNA, Control, Negative Control, Recombinant

KEY RESOURCES TABLE

Journal: Cell stem cell

Article Title: TFAP2C- and p63-Dependent Networks Sequentially Rearrange Chromatin Landscapes to Drive Human Epidermal Lineage Commitment

doi: 10.1016/j.stem.2018.12.012

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Scrambled negative control non-effective shRNA , Origene , Cat #TR30021.

Techniques: Purification, Recombinant, Knock-Out, Blocking Assay, Plasmid Preparation, Cloning, One Step RT-PCR, SYBR Green Assay, Magnetic Beads, Staining, Sequencing, CRISPR, Negative Control, shRNA, Expressing, Software