Journal: Frontiers in Nutrition

Article Title: Supplementation of Crataegi fructus alleviates functional dyspepsia and restores gut microbiota in mice

doi: 10.3389/fnut.2024.1385159

Figure Lengend Snippet: Hawthorn relieves symptoms of FD in mice. (A1) Body weight,♀; (A2) body weight,♂; (B1) food intake,♀; (B2) food intake,♂; (C1) water consumption,♀; (C2) water consumption,♂; (D) small intestinal propulsion rate; (E) intragastric residual rate; serum NO (F) and GAS (G) levels; VIP (H) , SP (I) , and 5-HT (J) levels in the duodenum. Data are presented as mean ± SD ( n = 10). * p < 0.05, ** p < 0.01.

Article Snippet: The 5-hydroxytryptamine (5-HT) ELISA Kit (Wuhan CUSABIO Co., Ltd., L211129838), the Mouse Substance P (SP) ELISA Kit (Wuhan CUSABIO Co., Ltd., L211012768), and the Mouse Vasoactive Intestinal Peptide (VIP) ELISA Kit (Wuhan CUSABIO Co., Ltd., L220113396) were used to determine the 5-HT, SP, and VIP levels in the duodenum.

Techniques:

Journal: bioRxiv

Article Title: Cellular- and systems-level profiling of amyloid-beta effects on circadian timing

doi: 10.64898/2025.12.22.695794

Figure Lengend Snippet: Coronal brain sections from 8 month-old WT and 5xFAD mice were immunolabeled for Aβ (white fluorescence) and two peptide markers of the SCN-arginine vasopressin (AVP-red fluorescence: Panel A) and vasoactive intestinal peptide (VIP-red fluorescence: Panel B)-and cellular nuclei were labeled with Hoechst (blue fluorescence). In A , high magnification images of the SCN are shown below each whole-brain coronal section. Representative images from 5xFAD tissue reveal marked Aβ-based plaque formation in a number of forebrain structures, including the cortex (CTX); However, plaque deposition was not observed in the SCN. Of note, AVP and VIP expression were indistinguishable between WT and 5xFAD mice, suggesting that the SCN was intact in 5xFAD animals. Bar for the low magnification image: 1000 microns; Bar for the high magnification image in A: 300 microns. C) Dot-blot protein profiling of 5xFAD and WT tissue indicates the presence of Aβ species in both the cortex and SCN of 5xFAD mice. As a control, lysates were also probed for β-actin. Further, the sensitivity of the Aβ antibody was confirmed by probing for recombinant Aβ.

Article Snippet: For fluorescence-based immunolabeling, 44 μm - thick tissue sections were initially permeabilized and blocked with PBS containing 0.02% Triton X-100 (v/v) and 10% bovine serum albumin (w/v) (1 hour), and then treated overnight with a rabbit anti-VIP antibody (1:2,000 dilution; Boster, Catalog code: RP1108) or a rabbit anti-AVP antibody (1:1,000 dilution: Millipore, Catalog code: AB1565).

Techniques: Immunolabeling, Fluorescence, Labeling, Expressing, Dot Blot, Control, Recombinant

Journal: The Journal of Neuroscience

Article Title: Chronic Mild Stress during Gestation Worsens Neonatal Brain Lesions in Mice

doi: 10.1523/JNEUROSCI.5330-06.2007

Figure Lengend Snippet: A–D, Effects of CUMS on excitotoxic brain lesions induced by ibotenate (A–D), NMDA (E, F), or S-bromowillardiine (G, H) in C57BL/6 mice. Excitotoxins were injected at P5, and pups were killed at P10. Bars represent mean sagittal diameter (A, C, E, G) or mean volume (B, D, F, H) of the lesions ± SEM. Asterisks indicate statistically significant difference from control males (A–D) or controls (E–H). *p < 0.05, **p < 0.01, ***p < 0.001 in three-way ANOVA with contrasts (A–G) or in Student's t test (E–H). Ctrl, Control.

Article Snippet: Excitotoxic lesions and lesion size determination Excitotoxic brain lesions were induced by injecting 10 μg of ibotenate (NMDA and metabotropic receptor agonist; Tocris Bioscience, Bristol, UK), 5 μg of NMDA (NMDA receptor agonist; Tocris Bioscience), or 15 μg of S-bromowillardiine (AMPA and kainate receptor agonist; Tocris Bioscience) into P5 mouse brains.

Techniques: Injection

Journal: The Journal of Neuroscience

Article Title: Chronic Mild Stress during Gestation Worsens Neonatal Brain Lesions in Mice

doi: 10.1523/JNEUROSCI.5330-06.2007

Figure Lengend Snippet: Real-time PCR quantification of glucocorticoid receptor (GR) mRNA. Pups from control (Ctrl) and GR-I unstressed B6C3F1 mothers were killed at P0 or P5. Brains were removed, and cortical plate (A) and underlying white matter (B) immediately adjacent to the normal site of excitotoxin injection were collected for RNA extraction. Bars represent mean GR/β2-microglobulin ratios ± SEM. Asterisks indicate statistically significant differences between Ctrl pups from unstressed mothers and pups from mothers exposed to CUMS. *p < 0.05, **p < 0.01, ***p < 0.001 in ANOVA with Dunnett's test.

Article Snippet: Excitotoxic lesions and lesion size determination Excitotoxic brain lesions were induced by injecting 10 μg of ibotenate (NMDA and metabotropic receptor agonist; Tocris Bioscience, Bristol, UK), 5 μg of NMDA (NMDA receptor agonist; Tocris Bioscience), or 15 μg of S-bromowillardiine (AMPA and kainate receptor agonist; Tocris Bioscience) into P5 mouse brains.

Techniques: Real-time Polymerase Chain Reaction, Injection, RNA Extraction