Journal:

Article Title: Anandamide-induced mobilization of cytosolic Ca 2+ in endothelial cells

doi: 10.1038/sj.bjp.0702483

Figure Lengend Snippet: (A) Concentration-dependent mobilization of cytosolic Ca2+ induced by anandamide in Ca2+-free physiological salt solution. Representative recordings that were obtained in fura 2-loaded EA.hy926 endothelial cell suspensions challenged with 0.1 μM (circles, n=5), 1 μM (squares, n=6) and 10 μM (diamonds, n=7) anandamide, respectively. 0.1 μM anandamide did not produce a significant release of Ca2+ from intracellular stores. (B) Representative recordings from EA.hy926 endothelial cell suspensions that were either not stimulated (bottom trace, diamonds, n=10) or stimulated with 100 μM histamine (top trace, squares, n=5) or 5 μM anandamide (middle trace, circles, n=11) in nominally Ca2+-free physiological. In all panels, horizontal bars indicate application of stimulants or readmission of Ca2+. (C) Representative recordings obtained in cells that were incubated with physiological salt solution containing 2.5 mM Ca2+, followed by a stimulation with either 5 μM anandamide (open circles, n=4) or 100 μM histamine (closed circles, n=4). Symbols represent mean±s.e.mean of fluorescence ratio values obtained at indicated times. *P<0.05 between changes in cytosolic Ca2+ in stimulated versus non-stimulated cells. Error bars not visible when the size of the symbol exceeds the spread of s.e.mean.

Article Snippet: [ PubMed ] [ Google Scholar ] DEUTSCH D.G., GOLIGORSKY, SCHMID P.C., KREBSAQCH R.J., SCHMID H.H.O., DAS S.K., KEY S.K., ARREAZA G., THORUP C., STEFANO G., PIOMELLI D. Production and physiological actions of anandamide in the vasculature of the rat kidney.

Techniques: Concentration Assay, Incubation, Fluorescence

Journal:

Article Title: Anandamide-induced mobilization of cytosolic Ca 2+ in endothelial cells

doi: 10.1038/sj.bjp.0702483

Figure Lengend Snippet: (A) Representative recordings from fura 2-loaded EA.hy926 endothelial cell suspensions that were stimulated with 100 μM histamine in the absence (continuous lines and open circles, n=4) and in the presence (dotted lines and closed circles, n=5) of 5 μM anandamide, applied as indicated by horizontal bars. Experiments were performed in Ca2+-free physiological salt solution. (B) Recordings obtained in cells that were stimulated with 5 μM anandamide in the absence (continuous lines and open circles, n=7) and in the presence (dotted lines and closed circles, n=7) of caffeine. Experiments were performed in Ca2+-free physiological salt solution. Symbols represent mean±s.e.mean of fluorescence ratio values obtained at indicated times. *P<0.05 between treatments. Error bars not visible when the size of the symbol exceeds the spread of s.e.mean.

Article Snippet: [ PubMed ] [ Google Scholar ] DEUTSCH D.G., GOLIGORSKY, SCHMID P.C., KREBSAQCH R.J., SCHMID H.H.O., DAS S.K., KEY S.K., ARREAZA G., THORUP C., STEFANO G., PIOMELLI D. Production and physiological actions of anandamide in the vasculature of the rat kidney.

Techniques: Fluorescence

Journal:

Article Title: Anandamide-induced mobilization of cytosolic Ca 2+ in endothelial cells

doi: 10.1038/sj.bjp.0702483

Figure Lengend Snippet: Endothelial NOS activity was determined in confluent EA.hy926 endothelial cells cultured in 6 well plastic dishes. The cells were challenged or not (basal) with 5 μM anandamide or 100 μM histamine, in the absence and in the presence of 300 μM L-NG-nitro-arginine, as detailed in methods. Activity of endothelial nitric oxide synthase was expressed as the mean±s.e.mean of the percentage of the L-NG-nitro-arginine-sensitive conversion of L-[3H-]-arginine to L-[3H]-citrulline. Error bar for anandamide was too small to be apparent. *P<0.05 between control and anandamide- or histamine-treated groups (n=6 each).

Article Snippet: [ PubMed ] [ Google Scholar ] DEUTSCH D.G., GOLIGORSKY, SCHMID P.C., KREBSAQCH R.J., SCHMID H.H.O., DAS S.K., KEY S.K., ARREAZA G., THORUP C., STEFANO G., PIOMELLI D. Production and physiological actions of anandamide in the vasculature of the rat kidney.

Techniques: Activity Assay, Cell Culture, Control

Journal:

Article Title: Anandamide-induced mobilization of cytosolic Ca 2+ in endothelial cells

doi: 10.1038/sj.bjp.0702483

Figure Lengend Snippet: Inhibition by SR141716A of anandamide-induced mobilization of cytosolic Ca2+. Recordings from fura 2-loaded endothelial cell suspensions that were stimulated with anandamide in the absence (continuous lines and open circles) and in the presence (dotted lines and closed circles) of either 1 μM (A) or 5 μM SR141716A (B). The cells were first equilibrated for 5 min with or without SR141716A in Ca2+-free physiological salt solution. Circles represent mean±s.e.mean of fluorescence ratio values obtained at indicated times. Error bars not visible when the size of the symbol exceeds the span of the s.e.mean. *P<0.05 between control and SR141716A-treated groups (n=4–6).

Article Snippet: [ PubMed ] [ Google Scholar ] DEUTSCH D.G., GOLIGORSKY, SCHMID P.C., KREBSAQCH R.J., SCHMID H.H.O., DAS S.K., KEY S.K., ARREAZA G., THORUP C., STEFANO G., PIOMELLI D. Production and physiological actions of anandamide in the vasculature of the rat kidney.

Techniques: Inhibition, Fluorescence, Control