Journal: bioRxiv

Article Title: Repurposing Antibacterial Drugs and Clinical Molecules to Combat Vancomycin-Resistant Enterococci (VRE)

doi: 10.1101/2025.03.27.645859

Figure Lengend Snippet: Time kill assay of ridinilazole, CRS3123 and linezolid at 5× and 10× MIC, against E. faecium NR-31909. Samples treated with DMSO were used as negative control. The results are given as means ± SD (n = 3; data without error bars indicate that the SD is too small to be seen).

Article Snippet: Linezolid (Selleck Chemicals) and vancomycin hydrochloride (Gold Biotechnology) were all purchased from commercial suppliers.

Techniques: Time-Kill Assay, Negative Control

Journal: bioRxiv

Article Title: Repurposing Antibacterial Drugs and Clinical Molecules to Combat Vancomycin-Resistant Enterococci (VRE)

doi: 10.1101/2025.03.27.645859

Figure Lengend Snippet: In vivo efficacy of ridinilazole and CRS3123 in a Caenorhabditis elegans model of bacterial infection. C. elegans were infected with VRE , E. faecium NR-31909. After infection, worms were treated with ridinilazole and CRS3123 or linezolid at 10× MIC. After 24 hours, worms were lysed, and bacteria were plated and CFU were counted after 24 hr. Results are expressed as means from three biological replicates ± standard deviation. Statistical analyses were determined by one-way ANOVA with post hoc testing (*** p <0.005), (**** p < 0.0001).

Article Snippet: Linezolid (Selleck Chemicals) and vancomycin hydrochloride (Gold Biotechnology) were all purchased from commercial suppliers.

Techniques: In Vivo, Infection, Bacteria, Standard Deviation

Journal: European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences

Article Title: In vitro release of hydrophobic drugs by oleogel rods with biocompatible gelators

doi: 10.1016/j.ejps.2020.105413

Figure Lengend Snippet: Summary of formulations used in drug release.

Article Snippet: The drug dexamethasone (purity: 99%), triamcinolone (purity 97%), and vancomycin HCL (vancomycin) (purity: 93.9%) were obtained from Carbosynth Limited.

Techniques: Solubility

Journal: Journal of Biological Chemistry

Article Title: Helix 8 of the Viral Chemokine Receptor ORF74 Directs Chemokine Binding

doi: 10.1074/jbc.m606877200

Figure Lengend Snippet: FIGURE 1. Expression of ORF74 deletion mutants. A, schematic representa- tion of the C-tail of ORF74-WT and mutants. Residues downstream of the conserved Pro at the end of TM7 (P7.50311) are shown. R7.61322 and Q7.62323 are shown in bold. The region conserved among -herpesvirus GPCRs is depicted in italics, and Hx8 is underlined. B, ELISA with HA-tagged. COS-7 cells were transfected with cDNA encoding N-terminally HA-tagged ORF74 con- structsoremptyvector.Forty-eighthoursaftertransfection,expressionofthe HA-tagged receptors was determined using an anti-HA antibody in an ELISA. Data are presented as the percentage of the absorption displayed by mock- transfected (mock) cells. A representative experiment performed in triplicate is shown. The experiment was repeated three times. C, confocal microscopy with EGFP-tagged ORF74. Representative pictures of COS-7 cells were made after transfection with C-terminally EGFP-tagged ORF74 constructs.

Article Snippet: After blocking with 1% skimmilk in 0.1 M NaHCO3 (pH 8.6), cells were incubated with mouse monoclonal anti-HA antibody (a gift from J. van Minnen) in Tris-buffered saline containing 0.1% bovine serum albumin, washed three times with Tris-buffered saline, and incubated with goat anti-mouse horseradish peroxidase-conjugated secondary antibody (Bio-Rad).

Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Transfection, Confocal Microscopy, Construct

Journal: ACS Omega

Article Title: Electrospun Chitosan-Based Nanofibrous Coating for the Local and Sustained Release of Vancomycin

doi: 10.1021/acsomega.3c08113

Figure Lengend Snippet: Microscopic characterization of nanofibrous mat SEM images of formulations of vancomycin nanofibers at magnifications of 5000x and corresponding size distribution histograms. (A) Formulation with chitosan in HFIP. (B) Formulation with chitosan and PVA in acetic acid solution (F2). (C) Formulation with chitosan and PEO in acetic acid solution (F4). Chitosan alone proved to be extremely difficult to spin due to its limited solubility (A), and for this reason, the addition of a copolymer such as PVA and PEO was explored to enhance the electrospinnability properties of chitosan (B,C).

Article Snippet: Vancomycin HCl was purchased from Tocris Bioscience (Minneapolis).

Techniques: Formulation, Solubility