Journal: iScience

Article Title: Loratadine inhibits Staphylococcus aureus virulence and biofilm formation

doi: 10.1016/j.isci.2022.103731

Figure Lengend Snippet:

Article Snippet: Vancomycin , MedChemExpress , HY-B0671; CAS: 1404-90-6.

Techniques: Recombinant, Analogues, Enzyme-linked Immunosorbent Assay, Sample Prep, Sequencing, Amplification, Over Expression, Plasmid Preparation, Clone Assay, Software

Journal: Gut Microbes

Article Title: Gut microbial bile acid metabolite skews macrophage polarization and contributes to high-fat diet-induced colonic inflammation

doi: 10.1080/19490976.2020.1819155

Figure Lengend Snippet: Vancomycin treatment changes gut microbiota and alleviates HFD-induced colonic pro-inflammatory macrophages infiltration. (a) Animal treatment procedure (ND, n = 5; HFD, n = 6; HFD+VCM, n = 6). (b) Stool samples were analyzed by 16S rDNA sequencing and the relative abundance of OTUs in the fecal bacterial community is shown. Data represent the mean of three mice per group. (c) Fecal DCA concentrations. (d) Representative HE and F4/80 staining as well as histological score of colon sections of differently treated mice (Scale bar, 50 µM). Quantification of IHC staining was exhibited as the number of F4/80 positive cells per field (n = 5). (e) Quantification of the number of goblet cells per mm 2 (upper panel) and lymphocytes per field (bottom panel). (f) Representative iNOS (red), F4/80 (green), CD206 (red) and DAPI (blue) immunofluorescence staining of colon tissues from ND, HFD and HFD plus vancomycin treated mice (Scale bar, 10 µM). The histograms indicate the percentages of iNOS or CD206 positive cells in macrophages (F4/80 + ). **: p < .01; ***: p < .001. Error bars indicate s.e.m.

Article Snippet: Vancomycin, QNZ and SKI-606 were obtained from Selleckchem (Houston, TX).

Techniques: Sequencing, Staining, Immunohistochemistry, Immunofluorescence