Journal: iScience

Article Title: A micro-electroporation/electrophoresis-based vaccine screening system reveals the impact of vaccination orders on cross-protective immunity

doi: 10.1016/j.isci.2023.108086

Figure Lengend Snippet:

Article Snippet: WT SARS-CoV-2 spike , Sino Biological , Cat #40589-V08B1.

Techniques: Virus, Recombinant, Cream, Enzyme-linked Immunosorbent Assay, Luciferase, Lysis, Staining, Membrane, Western Blot, Software

Journal: bioRxiv

Article Title: Amplified visual immunosensor integrated with nanozyme for ultrasensitive detection of avian influenza virus

doi: 10.1101/128538

Figure Lengend Snippet: Optical study of Au nanostructure and antibody specificity towards target virus. (A) X-ray diffraction spectra of Au nanostructure. (B) ELISA results of antibody specificity towards target virus.

Article Snippet: Influenza A (H5N2) hemagglutinin antibodies (Anti-H3N2 antibodies HA MAb, Lot: HB05AP2609), Influenza A (H7N9) hemagglutinin antibodies (Anti-H7N9 antibody HA MAb, Lot: HB05JA1903), recombinant influenza virus A (H5N2) HA1 (A/Ostrich/South Africa/A/109/2006)(lot: LC09AP1021), recombinant influenza virus A (H7N8) HA1 (A/Mallard/Netherlands/33/2006) (lot: LC09AP1323) and recombinant influenza virus A (H7N9) HA1 (A/Shanghai/1/2013) (lot: LC09JA2702) were purchased from Sino Biological, Inc. (Beijing, China).

Techniques: Enzyme-linked Immunosorbent Assay

Journal: bioRxiv

Article Title: Amplified visual immunosensor integrated with nanozyme for ultrasensitive detection of avian influenza virus

doi: 10.1101/128538

Figure Lengend Snippet: Detection of avian influenza virus A (H5N1). (A) The calibration curve of the absorbance corresponding to the concentration of avian influenza virus A (H5N1). BSA was used as a negative control. Squares (red line) and circles (black line) denote proposed and conventional ELISA sensing results, respectively. (B) ELISA results for selectivity of the present study with different influenza viruses. Error bars in (A) and (B) denote standard deviations (n=3).

Article Snippet: Influenza A (H5N2) hemagglutinin antibodies (Anti-H3N2 antibodies HA MAb, Lot: HB05AP2609), Influenza A (H7N9) hemagglutinin antibodies (Anti-H7N9 antibody HA MAb, Lot: HB05JA1903), recombinant influenza virus A (H5N2) HA1 (A/Ostrich/South Africa/A/109/2006)(lot: LC09AP1021), recombinant influenza virus A (H7N8) HA1 (A/Mallard/Netherlands/33/2006) (lot: LC09AP1323) and recombinant influenza virus A (H7N9) HA1 (A/Shanghai/1/2013) (lot: LC09JA2702) were purchased from Sino Biological, Inc. (Beijing, China).

Techniques: Concentration Assay, Negative Control, Enzyme-linked Immunosorbent Assay

Journal: Matter

Article Title: SARS-CoV-2 RapidPlex: A Graphene-Based Multiplexed Telemedicine Platform for Rapid and Low-Cost COVID-19 Diagnosis and Monitoring

doi: 10.1016/j.matt.2020.09.027

Figure Lengend Snippet: A Wireless Graphene-Based Telemedicine Platform (SARS-CoV-2 RapidPlex) for Rapid and Multiplex Electrochemical Detection of SARS-CoV-2 in Blood and Saliva (A) Schematic illustration of the SARS-CoV-2 RapidPlex multisensor telemedicine platform for detection of SARS-CoV-2 viral proteins, antibodies (IgG and IgM), and inflammatory biomarker C-reactive protein (CRP). Data can be wirelessly transmitted to a mobile user interface. WE, working electrode; CE, counter electrode; RE, reference electrode. (B) Mass-producible laser-engraved graphene sensor arrays. (C) Photograph of a disposable and flexible graphene array. (D) Image of a SARS-CoV-2 RapidPlex system with a graphene sensor array connected to a printed circuit board for signal processing and wireless communication.

Article Snippet: Mouse NP monoclonal antibody (mAb) (40143-MM05), SARS-CoV-2 NP antigen (40588-V08B), SARS-CoV/SARS-CoV-2 nucleocapsid antibody, rabbit mAb (40143-R001), SARS-CoV NP antigen (HCoV-OC43; 40643-V07E), SARS-CoV-2 Spike S1-His recombinant protein (HPLC-verified) (40591-V08H), and SARS-CoV Spike S1 protein (S1 subunit, His tag) (40150-V08B1) were purchased from Sino Biological.

Techniques: Multiplex Assay, Biomarker Assay

Journal: Matter

Article Title: SARS-CoV-2 RapidPlex: A Graphene-Based Multiplexed Telemedicine Platform for Rapid and Low-Cost COVID-19 Diagnosis and Monitoring

doi: 10.1016/j.matt.2020.09.027

Figure Lengend Snippet: Key Information on an Individual's COVID-19 Infection Status Provided by the SARS-CoV-2 RapidPlex

Article Snippet: Mouse NP monoclonal antibody (mAb) (40143-MM05), SARS-CoV-2 NP antigen (40588-V08B), SARS-CoV/SARS-CoV-2 nucleocapsid antibody, rabbit mAb (40143-R001), SARS-CoV NP antigen (HCoV-OC43; 40643-V07E), SARS-CoV-2 Spike S1-His recombinant protein (HPLC-verified) (40591-V08H), and SARS-CoV Spike S1 protein (S1 subunit, His tag) (40150-V08B1) were purchased from Sino Biological.

Techniques: Infection

Journal: Matter

Article Title: SARS-CoV-2 RapidPlex: A Graphene-Based Multiplexed Telemedicine Platform for Rapid and Low-Cost COVID-19 Diagnosis and Monitoring

doi: 10.1016/j.matt.2020.09.027

Figure Lengend Snippet: Characterization of Electrochemical Graphene Biosensors Comprising the SARS-CoV-2 RapidPlex Platform (A) Scheme detailing the methodology developed for the covalent attachment of the corresponding bioreceptor for the specific capture of the target analytes SARS-CoV-2 NP and CRP (left), and IgG and IgM isotypes against SARS-CoV-2 S1 protein (right). PBA, 1-pyrenebutyric acid; BSA, bovine serum albumin; CAb, capture antibody; PI, polyimide. (B and C) Differential pulse voltammetry (B) and Nyquist plots (C) of a graphene electrode in 0.01 M PBS (pH 7.4) containing 2.0 mM K 4 Fe(CN) 6 /K 3 Fe(CN) 6 (1:1) after each modification step (S1-IgG assay as representative example): bare graphene (Bare), functionalization with PBA (PBA), immobilization of SARS-CoV-2 S1 protein (Protein), blocking with BSA (BSA), recognition of specific S1-IgG (Target), and incubation with enzyme-tagged anti-human IgG antibody (DAb). (D) Comparison of amperometric responses and overlaid signal-to-blank (S/B) ratio (black lines) for SARS-CoV-2-specific IgG and CRP detection using PBA and 1H-pyrrole-1-propionic acid (PPA) as linkers for the attachment of the corresponding capture bioreceptors. Data are presented as mean ± SD (n = 3). (E) Amperometric responses and overlaid S/B ratio (black lines) observed for 0.0 and 500 pg mL −1 NP, 0.0 and 250 ng mL −1 SARS-CoV-2-specific IgG and IgM, and 0.0 and 50 ng mL −1 CRP, with 1-, 5-, and 10-min incubation. Data are presented as mean ± SD (n = 3).

Article Snippet: Mouse NP monoclonal antibody (mAb) (40143-MM05), SARS-CoV-2 NP antigen (40588-V08B), SARS-CoV/SARS-CoV-2 nucleocapsid antibody, rabbit mAb (40143-R001), SARS-CoV NP antigen (HCoV-OC43; 40643-V07E), SARS-CoV-2 Spike S1-His recombinant protein (HPLC-verified) (40591-V08H), and SARS-CoV Spike S1 protein (S1 subunit, His tag) (40150-V08B1) were purchased from Sino Biological.

Techniques: Modification, Blocking Assay, Incubation

Journal: Matter

Article Title: SARS-CoV-2 RapidPlex: A Graphene-Based Multiplexed Telemedicine Platform for Rapid and Low-Cost COVID-19 Diagnosis and Monitoring

doi: 10.1016/j.matt.2020.09.027

Figure Lengend Snippet: Evaluation of Analytical Sensor Performance for the Detection of Physiological Levels of Target COVID-19 Biomarkers (A) Scheme of sensor preparation for detection of SARS-CoV-2 NP and CRP based on double-sandwich and sandwich assay configurations, respectively. CAb, capture antibody; DAb, detector antibody; DAb 2 , secondary detector antibody; HRP, horseradish peroxidase. (B and C) Calibration curves constructed for NP (B) and CRP (C) detection in PBS (pH 7.4) supplemented with 1.0% BSA. Data are presented as mean ± SD (n = 3). (D) Scheme of sensor preparation for detection of S1-IgG and S1-IgM isotypes based on direct assay configurations. (E and F) Calibration curves constructed for S1-IgG (E) and S1-IgM (F) isotype detection in PBS (pH 7.4) supplemented with 1.0% BSA. Data are presented as mean ± SD (n = 3).

Article Snippet: Mouse NP monoclonal antibody (mAb) (40143-MM05), SARS-CoV-2 NP antigen (40588-V08B), SARS-CoV/SARS-CoV-2 nucleocapsid antibody, rabbit mAb (40143-R001), SARS-CoV NP antigen (HCoV-OC43; 40643-V07E), SARS-CoV-2 Spike S1-His recombinant protein (HPLC-verified) (40591-V08H), and SARS-CoV Spike S1 protein (S1 subunit, His tag) (40150-V08B1) were purchased from Sino Biological.

Techniques: Construct

Journal: Matter

Article Title: SARS-CoV-2 RapidPlex: A Graphene-Based Multiplexed Telemedicine Platform for Rapid and Low-Cost COVID-19 Diagnosis and Monitoring

doi: 10.1016/j.matt.2020.09.027

Figure Lengend Snippet: Investigation of the Selectivity and Multiplexed Performance of the Wireless SARS-CoV-2 RapidPlex Platform (A) Selective response of NP, S1-IgG and S1-IgM isotypes, and CRP sensors against different non-target circulating analytes. Interferential molecules were tested at 500 pg mL −1 (with an exception of 50 ng mL −1 for CRP), 250 ng mL −1 , and 50 ng mL −1 for NP, S1-IgG and S1-IgM, and CRP assays, respectively. Data are presented as mean ± SD (n = 3). (B) Validation of sample concentrations measured using the designed electrochemical sensor against sample concentrations measured using ELISA. (C) Block diagram of the SARS-CoV-2 RapidPlex platform. UART, universal asynchronous receiver/transmitter; MCU, microcontroller unit; DAC, digital-to-analog converter; ADC, analog-to-digital converter. (D) Schematic illustration of the graphene sensor array layout. (E) Experimental readings obtained with the functionalized SARS-CoV-2 RapidPlex platform after incubation of the four WEs with PBS (pH 7.4) supplemented with 1.0% BSA containing 1.0 ng mL −1 NP (I), 250 ng mL −1 S1-IgG (II), 250 ng mL −1 S1-IgM (III), and 50 ng mL −1 CRP (IV).

Article Snippet: Mouse NP monoclonal antibody (mAb) (40143-MM05), SARS-CoV-2 NP antigen (40588-V08B), SARS-CoV/SARS-CoV-2 nucleocapsid antibody, rabbit mAb (40143-R001), SARS-CoV NP antigen (HCoV-OC43; 40643-V07E), SARS-CoV-2 Spike S1-His recombinant protein (HPLC-verified) (40591-V08H), and SARS-CoV Spike S1 protein (S1 subunit, His tag) (40150-V08B1) were purchased from Sino Biological.

Techniques: Enzyme-linked Immunosorbent Assay, Blocking Assay, Incubation

Journal: Matter

Article Title: SARS-CoV-2 RapidPlex: A Graphene-Based Multiplexed Telemedicine Platform for Rapid and Low-Cost COVID-19 Diagnosis and Monitoring

doi: 10.1016/j.matt.2020.09.027

Figure Lengend Snippet: Application of SARS-CoV-2 RapidPlex in SARS-CoV-2 Detection in Blood and Saliva Samples from COVID-19-Positive and -Negative Subjects (A and B) Experimental readings obtained with SARS-CoV-2 RapidPlex after 10-min incubation of the sensor array with serum samples from a representative COVID-19 RT-PCR-negative (A) and -positive (B) patient. (C) Signal of individual sensor obtained after 1-min incubation with a serum sample from a COVID-19-positive patient (dark color) versus the signal obtained after 10-min incubation with a serum sample from a COVID-19-negative patient (light color). (D) Box-and-whisker plot of measured signal-to-blank ratios (S/B) for NP, S1-IgG, S1-IgM, and CRP in RT-PCR-confirmed COVID-19-positive (n = 5) and -negative (n = 6) serum samples. (E) Box-and-whisker plot of measured S/B for NP, S1-IgG, S1-IgM, and CRP in RT-PCR-confirmed COVID-19-positive (n = 5) and -negative (n = 3) saliva samples. (F) CRP levels in diluted serum samples plotted against given COVID-19 symptom severity, with “Healthy” referring to COVID-19-negative patient samples (n = 7). Positive COVID-19 patients were classified according to disease severity as asymptomatic (n = 2), mild (n = 5), and moderate (n = 2).

Article Snippet: Mouse NP monoclonal antibody (mAb) (40143-MM05), SARS-CoV-2 NP antigen (40588-V08B), SARS-CoV/SARS-CoV-2 nucleocapsid antibody, rabbit mAb (40143-R001), SARS-CoV NP antigen (HCoV-OC43; 40643-V07E), SARS-CoV-2 Spike S1-His recombinant protein (HPLC-verified) (40591-V08H), and SARS-CoV Spike S1 protein (S1 subunit, His tag) (40150-V08B1) were purchased from Sino Biological.

Techniques: Incubation, Reverse Transcription Polymerase Chain Reaction, Whisker Assay

Journal: Cell host & microbe

Article Title: Longitudinal human antibody repertoire against complete viral proteome from Ebola virus survivor reveals protective sites for vaccine design

doi: 10.1016/j.chom.2020.01.001

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Recombinant Mayinga 1976 EBOV GP , Sino Biologicals , 40304-V08B1.

Techniques: Recombinant