Journal: Acta pharmaceutica Sinica. B

Article Title: NAMPT-targeting PROTAC promotes antitumor immunity via suppressing myeloid-derived suppressor cell expansion.

doi: 10.1016/j.apsb.2021.12.017

Figure Lengend Snippet: Figure 1 NAMPT promotes the expansion of MDSCs independent of its enzymatic activity. (A) Immunoblot analysis of iNAMPT (intracellular NAMPT) and eNAMPT (extracellular NAMPT). (B) Cell growth of CT26 cells with stable knockdown of NAMPT. Left, CT26 scramble control (shNC) or shNAMPT cell confluency assessed by incuCyte proliferation assay; Right, immunoblot analysis of NAMPT. (C) Tumor growth curve. CT26 shNC control or shNAMPT cells were inoculated subcutaneously in BALB/c mice (n Z 10). (DeH) Tumor infiltrating immune cells analyzed by flow cytometry. CT26 shNC control or shNAMPT cells were inoculated subcutaneously in BALB/c mice (n Z 15). (D) Heatmap showing the proportion of the indicated immune cells in tumor infiltrating CD45þ cells. (E) MDSCs; (F) CD8þ T cells; (G) IFNgþ CD8þ T cells; (H) Granzyme Bþ CD8þ T cells. (I) In vitro MDSC differentiation assay. Left, the schematic illustration of co-culture experiment. MC38 scramble control (shNC) or shNAMPT cells were co-cultured with mice bone marrow. Right, normalized counts of GR1high CD11bþ cells analyzed by flow cytometry. (J) Signal transducer and activator of transcription 3 (STAT3) signaling change in MDSCs. MDSCs from murine bone marrow were treated with recombinant NAMPT (200 ng) for 2 h and immunoblotting analysis was performed. (K) Proportion of MDSCs in tumor infiltrating CD45þ cells. CT26 tumor-bearing mice were treated with vehicle control or FK866 (16 mg/kg, daily) for 5 days (n Z 5). All data depict mean SEM. n.s., not significant. *P < 0.05, **P < 0.01, ***P < 0.001.

Article Snippet: For live cell imaging, cell plates were put into the cell incubator of Incucyte Live-Cell Analysis System (Essen Bioscience), images were captured every 6 h for 4 consecutive days, and the cell growth curves were generated.

Techniques: Activity Assay, Western Blot, Knockdown, Control, Proliferation Assay, Cytometry, In Vitro, Differentiation Assay, Co-Culture Assay, Cell Culture, Recombinant

Journal: Micromachines

Article Title: A Novel 3D-Printed and Miniaturized Periodic Counter Current Chromatography System for Continuous Purification of Monoclonal Antibodies

doi: 10.3390/mi15030382

Figure Lengend Snippet: Chromatogram of a continuous membrane adsorber-based CEX run using three membrane adsorbers numbered MA1–MA3. A total of 4 cycles (55 min) and a cleansing post cycle using membrane adsorbers with a volume of 0.41 mL were performed. Small regular plateaus with peaks at the end emerge during loading.

Article Snippet: The consecutive polishing step was performed via cation exchange chromatography using membrane adsorbers (Sartobind ® S15, Sartorius Stedim Biotech, Göttingen, Germany).

Techniques: Membrane