Journal: Nutrients

Article Title: Wheat Extract Oil (WEO) Attenuates UVB-Induced Photoaging via Collagen Synthesis in Human Keratinocytes and Hairless Mice

doi: 10.3390/nu12020300

Figure Lengend Snippet: WEO-induced changes in skin surface condition at week 12. ( A ) Skin moisture measurement by Corneometer CM825. ( B ) Transepidermal water loss measurement by Tewameter TM 300. ( C ) Skin elasticity measurement by Cutometer MPA580. ( D ) Roughness measurement by Visioscan VC98. Values with different letters are significantly different by analysis of variance (ANOVA) followed by the Newman– Keuls multiple range test ( p < 0.05). NC: normal control, UVB: Ultraviolet, WEO: wheat extract oil, HA: Hyaluronic acid.

Article Snippet: An ultraviolet irradiation system (LK3500) fitted with a UVB lamp (5 Sankyo Denki G5T5E lamps, Sankyo Denki Co., Kanawaga, Japan) was used to induce skin damage to simulate photoaging.

Techniques: Control

Journal: Nutrients

Article Title: Wheat Extract Oil (WEO) Attenuates UVB-Induced Photoaging via Collagen Synthesis in Human Keratinocytes and Hairless Mice

doi: 10.3390/nu12020300

Figure Lengend Snippet: Effect of WEO on skin tissue components at 12 weeks. ( A ) Procollagen type I measurement by ELISA. ( B ) Hyaluronic acid measurement by ELISA. ( C ) Ceramide measurement by LC/MS/MS. ( D ) Collagen measurement by Masson’s trichrome stain. Values with different letters are significantly different by ANOVA followed by the Newman–Keuls multiple range test ( p < 0.05). NC: normal control, UVB: Ultraviolet, WEO: wheat extract oil, HA: Hyaluronic acid.

Article Snippet: An ultraviolet irradiation system (LK3500) fitted with a UVB lamp (5 Sankyo Denki G5T5E lamps, Sankyo Denki Co., Kanawaga, Japan) was used to induce skin damage to simulate photoaging.

Techniques: Enzyme-linked Immunosorbent Assay, Liquid Chromatography with Mass Spectroscopy, Staining, Control

Journal: Nutrients

Article Title: Wheat Extract Oil (WEO) Attenuates UVB-Induced Photoaging via Collagen Synthesis in Human Keratinocytes and Hairless Mice

doi: 10.3390/nu12020300

Figure Lengend Snippet: Epidermis thickness measurement (Masson’s trichome stain) at week 12. Values with different letters are significantly different by ANOVA followed by the Newman–Keuls multiple range test ( p < 0.05). NC: normal control, UVB: Ultraviolet, WEO: wheat extract oil, HA: Hyaluronic acid.

Article Snippet: An ultraviolet irradiation system (LK3500) fitted with a UVB lamp (5 Sankyo Denki G5T5E lamps, Sankyo Denki Co., Kanawaga, Japan) was used to induce skin damage to simulate photoaging.

Techniques: Staining, Control

Journal: Nutrients

Article Title: Wheat Extract Oil (WEO) Attenuates UVB-Induced Photoaging via Collagen Synthesis in Human Keratinocytes and Hairless Mice

doi: 10.3390/nu12020300

Figure Lengend Snippet: Changes in skin-damage-related factors in human skin cell lines. ( A ) Procollagen synthetic enhancement measurement in CCD-986sk cells by microplate reader. ( B ) Hyaluronic acid measurement in HaCaT cells. ( C ) Measurement of matrix metalloproteinase-1 (MMP-1) mRNA expression in CCD-986sk cells by qRT-PCR. Values with different letters are significantly different by ANOVA followed by the Newman–Keuls multiple range test ( p < 0.05). NC: normal control, UVB: Ultraviolet, WEO: wheat extract oil, HA: Hyaluronic acid.

Article Snippet: An ultraviolet irradiation system (LK3500) fitted with a UVB lamp (5 Sankyo Denki G5T5E lamps, Sankyo Denki Co., Kanawaga, Japan) was used to induce skin damage to simulate photoaging.

Techniques: Expressing, Quantitative RT-PCR, Control

Journal: Stem Cells and Development

Article Title: Ultraviolet B Preconditioning Enhances the Hair Growth-Promoting Effects of Adipose-Derived Stem Cells Via Generation of Reactive Oxygen Species

doi: 10.1089/scd.2012.0167

Figure Lengend Snippet: Effect of UVB irradiation on the proliferation and survival of ASCs. (A) Proliferation was measured 72 h after UVB irradiation using MTT assay. A low-dose UVB exposure did not affect, while a high dose reduced the ASC proliferation. (B) H2O2 treatment (0.5 mM for 24 h) induced the cell death, but low-dose UVB preconditioning increased ASC survival. (C) H2O2 treatment enhanced the PI staining of ASC nuclei, which was attenuated by UVB irradiation. (D) Western blot analysis showed that the phosphorylation of survival-related signaling molecules was significantly increased by UVB treatment. **P<0.01. UVB, ultraviolet B; ASCs, adipose-derived stem cells; H2O2, hydrogen peroxide; PI, propidium iodide. Color images available online at www.liebertpub.com/scd

Article Snippet: UVB preconditioning The UVB irradiation apparatus employed herein (BLE-1T158) was obtained from Spectronics Corp. A Kodacel filter (TA401/407; Kodak) was used to eliminate light wavelengths of less than 290 nm (corresponding to ultraviolet C radiation).

Techniques: Irradiation, MTT Assay, Staining, Western Blot, Phospho-proteomics, Derivative Assay

Journal: Stem Cells and Development

Article Title: Ultraviolet B Preconditioning Enhances the Hair Growth-Promoting Effects of Adipose-Derived Stem Cells Via Generation of Reactive Oxygen Species

doi: 10.1089/scd.2012.0167

Figure Lengend Snippet: Effect of UVB preconditioning on migration and tube-forming activities. (A, B) A low dose of UVB significantly increased the migration of ASCs in chamber (A) and cell scratch (B) migration assays. (C) Tube formation on Matrigel was enhanced by UVB irradiation, and tube length was measured. (D) In angiogenesis chip array, protein expression of coagulation factor III, uPA, VEGF, and HGF was upregulated in EGM2 after UVB radiation (gray bars: control, black bars: UVB treatment). *P<0.05; **P<0.01. uPA, urokinase-type plasminogen activator; VEGF, vascular endothelial growth factor; HGF, hepatocyte growth factor; EGM2, endothelial growth medium.

Article Snippet: UVB preconditioning The UVB irradiation apparatus employed herein (BLE-1T158) was obtained from Spectronics Corp. A Kodacel filter (TA401/407; Kodak) was used to eliminate light wavelengths of less than 290 nm (corresponding to ultraviolet C radiation).

Techniques: Migration, Irradiation, Expressing, Coagulation, Control

Journal: Stem Cells and Development

Article Title: Ultraviolet B Preconditioning Enhances the Hair Growth-Promoting Effects of Adipose-Derived Stem Cells Via Generation of Reactive Oxygen Species

doi: 10.1089/scd.2012.0167

Figure Lengend Snippet: Induced telogen-to-anagen transition by UVB preconditioning. After UVB irradiation, cells (A, B) or CM (C, D) were injected on the back of C3H/HeN mice. (A) Compared with untreated control ASCs, UVB treatment (ASCUVB) significantly accelerated the telogen-to-anagen transition at 14 days. (B) In addition, hair weight was significantly increased in the ASCUVB group at 18 days (n=5 for each group). (C) Compared with ASC-CM, subcutaneous injection of UVB-CM significantly induced the telogen-to-anagen transition at 14 days. (D) Hair weight was significantly increased in the UVB-CM injection group at 14 days (n=5 for each group). **P<0.01.

Article Snippet: UVB preconditioning The UVB irradiation apparatus employed herein (BLE-1T158) was obtained from Spectronics Corp. A Kodacel filter (TA401/407; Kodak) was used to eliminate light wavelengths of less than 290 nm (corresponding to ultraviolet C radiation).

Techniques: Irradiation, Injection, Control

Journal: Molecules

Article Title: Effects of Sphingomyelin-Containing Milk Phospholipids on Skin Hydration in UVB-Exposed Hairless Mice

doi: 10.3390/molecules27082545

Figure Lengend Snippet: Effects of milk phospholipids on erythema formation ( A ), skin thickness ( B ), skin hydration ( C ) and transepidermal water loss (TEWL) ( D ) in ultraviolet (UV) B-irradiated hairless mice. NOR: oral administration of saline without UVB irradiation; UVB-C: oral administration of saline under UVB irradiation; ML: oral administration of low-dose (50 mg/kg b.w.) milk phospholipids under UVB irradiation; MM: oral administration of medium-dose (100 mg/kg b.w.) milk phospholipids under UVB irradiation; MH: oral administration of high-dose (150 mg/kg b.w.) milk phospholipids under UVB irradiation. Data are expressed as means ± standard error ( n = 6). * p < 0.05, ** p < 0.01, and *** p < 0.001 vs. UVB-C group (Tukey’s test).

Article Snippet: For UV irradiation, a UV irradiator (BLX-254, Vilber Lourmat, Marne La Vallee, France) with UVB lamp (UB800, Waldman Licht Technik GmbH) was used.

Techniques: Irradiation

Journal: Molecules

Article Title: Effects of Sphingomyelin-Containing Milk Phospholipids on Skin Hydration in UVB-Exposed Hairless Mice

doi: 10.3390/molecules27082545

Figure Lengend Snippet: Effects of milk phospholipids on gene expression of HAS ( A – C ) and HYAL ( D , E ) and hyaluronic acid (HA) content ( F ) in UVB-irradiated hairless mice. NOR: oral administration of saline without UVB irradiation; UVB-C: oral administration of saline under UVB irradiation; ML: oral administration of low-dose (50 mg/kg b.w.) milk phospholipids under UVB irradiation; MM: oral administration of medium-dose (100 mg/kg b.w.) milk phospholipids under UVB irradiation; MH: oral administration of high-dose (150 mg/kg b.w.) milk phospholipids under UVB irradiation. Data are expressed as means ± standard error ( n = 6). * p < 0.05, ** p < 0.01, and *** p < 0.001 vs. UVB-C group (Tukey’s test). HAS: hyaluronan synthase; HYAL: hyaluronidase.

Article Snippet: For UV irradiation, a UV irradiator (BLX-254, Vilber Lourmat, Marne La Vallee, France) with UVB lamp (UB800, Waldman Licht Technik GmbH) was used.

Techniques: Expressing, Irradiation

Journal: Molecules

Article Title: Effects of Sphingomyelin-Containing Milk Phospholipids on Skin Hydration in UVB-Exposed Hairless Mice

doi: 10.3390/molecules27082545

Figure Lengend Snippet: Effects of milk phospholipids on the expression of filaggrin ( A ), involucrin ( B ), and AQP3 ( C ) in UVB-irradiated hairless mice. NOR: oral administration of saline without UVB irradiation; UVB-C: oral administration of saline under UVB irradiation; ML: oral administration of low-dose (50 mg/kg b.w.) milk phospholipids under UVB irradiation; MM: oral administration of medium-dose (100 mg/kg b.w.) milk phospholipids under UVB irradiation; MH: oral administration of high-dose (150 mg/kg b.w.) milk phospholipids under UVB irradiation. Data are expressed as means ± standard error ( n = 6). ** p < 0.01 vs. UVB-C group (Tukey’s test). AQP3: aquaporin3.

Article Snippet: For UV irradiation, a UV irradiator (BLX-254, Vilber Lourmat, Marne La Vallee, France) with UVB lamp (UB800, Waldman Licht Technik GmbH) was used.

Techniques: Expressing, Irradiation

Journal: Molecules

Article Title: Effects of Sphingomyelin-Containing Milk Phospholipids on Skin Hydration in UVB-Exposed Hairless Mice

doi: 10.3390/molecules27082545

Figure Lengend Snippet: Effects of milk phospholipids on ROS production ( A ) and the expression of genes encoding the antioxidant enzymes SOD1 ( B ), SOD2 ( C ), CAT ( D ), and GPx1 ( E ) in UVB-irradiated hairless mice. NOR: oral administration of saline without UVB irradiation; UVB-C: oral administration of saline under UVB irradiation; ML: oral administration of low-dose (50 mg/kg b.w.) milk phospholipids under UVB irradiation; MM: oral administration of medium-dose (100 mg/kg b.w.) milk phospholipids under UVB irradiation; MH: oral administration of high-dose (150 mg/kg b.w.) milk phospholipids under UVB irradiation. Data are expressed as means ± standard error ( n = 6). ** p < 0.01 and *** p < 0.001 vs. UVB-C group (Tukey’s test). ROS: reactive oxygen species; CAT: catalase; SOD: superoxide dismutase; Gpx-1: glutathione peroxidase-1.

Article Snippet: For UV irradiation, a UV irradiator (BLX-254, Vilber Lourmat, Marne La Vallee, France) with UVB lamp (UB800, Waldman Licht Technik GmbH) was used.

Techniques: Expressing, Irradiation

Journal: Molecules

Article Title: Effects of Sphingomyelin-Containing Milk Phospholipids on Skin Hydration in UVB-Exposed Hairless Mice

doi: 10.3390/molecules27082545

Figure Lengend Snippet: Effects of milk phospholipids on protein expression of Nrf2 ( A ), Keap1 ( B ), and HO-1 ( C ) in UVB-irradiated hairless mice. Western blot and protein quantifications are shown. NOR: oral administration of saline without UVB irradiation; UVB-C: oral administration of saline under UVB irradiation; ML: oral administration of low-dose (50 mg/kg b.w.) milk phospholipids under UVB irradiation; MM: oral administration of medium-dose (100 mg/kg b.w.) milk phospholipids under UVB irradiation; MH: oral administration of high-dose (150 mg/kg b.w.) milk phospholipids under UVB irradiation. Data are expressed as means ± standard error ( n = 6). * p < 0.05 and ** p < 0.01 vs. UVB-C group (Tukey’s test). Nrf2: Nuclear factor erythroid-2-related factor 2; Keap1: Kelch-like ECH-associated protein 1; HO-1: heme oxygenase-1.

Article Snippet: For UV irradiation, a UV irradiator (BLX-254, Vilber Lourmat, Marne La Vallee, France) with UVB lamp (UB800, Waldman Licht Technik GmbH) was used.

Techniques: Expressing, Irradiation, Western Blot