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Image Search Results
Journal: Stem Cells and Development
Article Title: Ultraviolet B Preconditioning Enhances the Hair Growth-Promoting Effects of Adipose-Derived Stem Cells Via Generation of Reactive Oxygen Species
doi: 10.1089/scd.2012.0167
Figure Lengend Snippet: Effect of UVB irradiation on the proliferation and survival of ASCs. (A) Proliferation was measured 72 h after UVB irradiation using MTT assay. A low-dose UVB exposure did not affect, while a high dose reduced the ASC proliferation. (B) H2O2 treatment (0.5 mM for 24 h) induced the cell death, but low-dose UVB preconditioning increased ASC survival. (C) H2O2 treatment enhanced the PI staining of ASC nuclei, which was attenuated by UVB irradiation. (D) Western blot analysis showed that the phosphorylation of survival-related signaling molecules was significantly increased by UVB treatment. **P<0.01. UVB, ultraviolet B; ASCs, adipose-derived stem cells; H2O2, hydrogen peroxide; PI, propidium iodide. Color images available online at www.liebertpub.com/scd
Article Snippet: UVB preconditioning The
Techniques: Irradiation, MTT Assay, Staining, Western Blot, Phospho-proteomics, Derivative Assay
Journal: Stem Cells and Development
Article Title: Ultraviolet B Preconditioning Enhances the Hair Growth-Promoting Effects of Adipose-Derived Stem Cells Via Generation of Reactive Oxygen Species
doi: 10.1089/scd.2012.0167
Figure Lengend Snippet: Effect of UVB preconditioning on migration and tube-forming activities. (A, B) A low dose of UVB significantly increased the migration of ASCs in chamber (A) and cell scratch (B) migration assays. (C) Tube formation on Matrigel was enhanced by UVB irradiation, and tube length was measured. (D) In angiogenesis chip array, protein expression of coagulation factor III, uPA, VEGF, and HGF was upregulated in EGM2 after UVB radiation (gray bars: control, black bars: UVB treatment). *P<0.05; **P<0.01. uPA, urokinase-type plasminogen activator; VEGF, vascular endothelial growth factor; HGF, hepatocyte growth factor; EGM2, endothelial growth medium.
Article Snippet: UVB preconditioning The
Techniques: Migration, Irradiation, Expressing, Coagulation, Control
Journal: Stem Cells and Development
Article Title: Ultraviolet B Preconditioning Enhances the Hair Growth-Promoting Effects of Adipose-Derived Stem Cells Via Generation of Reactive Oxygen Species
doi: 10.1089/scd.2012.0167
Figure Lengend Snippet: Induced telogen-to-anagen transition by UVB preconditioning. After UVB irradiation, cells (A, B) or CM (C, D) were injected on the back of C3H/HeN mice. (A) Compared with untreated control ASCs, UVB treatment (ASCUVB) significantly accelerated the telogen-to-anagen transition at 14 days. (B) In addition, hair weight was significantly increased in the ASCUVB group at 18 days (n=5 for each group). (C) Compared with ASC-CM, subcutaneous injection of UVB-CM significantly induced the telogen-to-anagen transition at 14 days. (D) Hair weight was significantly increased in the UVB-CM injection group at 14 days (n=5 for each group). **P<0.01.
Article Snippet: UVB preconditioning The
Techniques: Irradiation, Injection, Control