Journal: The Journal of Biological Chemistry

Article Title: Basis of Miscoding of the DNA Adduct N 2 ,3-Ethenoguanine by Human Y-family DNA Polymerases *

doi: 10.1074/jbc.M112.403253

Figure Lengend Snippet: Collision-induced dissociation spectra from LC-MS/MS analysis of the full-length extension assays using pol ι and a 23-mer oligomer template containing 2′-F-N2,3-ϵdG in the presence of all four dNTPs. A, template and primer sequences. The confirmed product sequences with the fragmentation patterns shown are 5′-CCATGA-3′ (B), 5′-CTATGA-3′ (C), 5′-CAATGA-3′ (D), 5′-CCATGAA-3′ (E), and 5′-CTATGAA-3′ (F). The reaction contained 12.5 μm DNA complex, 10 mm dNTPs, 10 μm pol ι, 5 mm DTT, 50 mm NaCl, 5 mm MgCl2, and 50 μg ml−1 BSA and were incubated at 37 °C for 3.5 h. Underlined U indicates the cutting site by uracil-DNA glycosylase after DNA polymerase reactions.

Article Snippet: Unlabeled dNTPs, T4 polynucleotide kinase, uracil-DNA glycosylase, and restriction endonucleases were from New England Biolabs (Ipswich, MA).

Techniques: Liquid Chromatography with Mass Spectroscopy, Incubation

Journal: Cell

Article Title: A Paleogenomic Reconstruction of the Deep Population History of the Andes

doi: 10.1016/j.cell.2020.04.015

Figure Lengend Snippet:

Article Snippet: UGI , New England Biolabs , M0281.

Techniques: Recombinant, Hybridization, SYBR Green Assay, Purification, Software, Sequencing