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Image Search Results
Journal: Molecular cell
Article Title: RNF8- and Ube2S-dependent ubiquitin lysine 11-linkage modification in response to DNA damage
doi: 10.1016/j.molcel.2017.04.013
Figure Lengend Snippet: U2OS cells were transfected with control siRNA or siRNAs targeting Ube2S/Ube2C. 48 hr post-transfection, cells were irradiated with 10 Gy IR followed by 2 hr incubation at 37°C followed by immunostaining with antibodies to conjugated ubiquitin (FK2 antibody) (A), 53BP1 (B), BRCA1 (C), Abraxas (D), or Rap80 (E). Percentage of foci-positive cells (cells containing more than 10 foci) was quantified and indicated as mean ± SD with p-value indicated.
Article Snippet: Antibodies used in this study are BRCA1 (Santa Cruz), γ-tubulin (Sigma); HA (Cell Signaling), γH2AX (Upstate),
Techniques: Transfection, Control, Irradiation, Incubation, Immunostaining, Ubiquitin Proteomics