u87392 Search Results


u87392  (ATCC)
93
ATCC u87392
U87392, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
u87392 - by Bioz Stars, 2026-03
93/100 stars
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reference strain vr2332  (ATCC)
95
ATCC reference strain vr2332
Experimental layout. Lungs were collected from influenza- and PRRSV-negative 8–12-week-old pigs. Mononuclear phagocytes (MNPs) from bronchoalveolar lavage (BAL) and parenchyma (PAR) were enriched using OptiPrep gradient. MNPs were infected with North Carolina (NC) PRRSV-2 strains NC134 and NC174 (MOI = 1) and control media for 12 h. Infected and control porcine alveolar macrophages (PAMs), pulmonary intravascular macrophages (PIMs), monocyte-derived dendritic cells (moDCs), and classical DCs (cDCs) were sorted, and RNA-seq was performed on each cell subset. Additional PAM and PIM sorted cells were used for qPCR and NanoString validations. For other assays, enriched MNPs were plated to allow macrophage adhesion for 2 h. PAMs and PIMs were subsequently infected with different NC PRRSV-2 strains (NC134, NC174, and NC144) or <t>VR2332</t> prototype strain; uninfected cells were used as negative control. Infected macrophages were used in different assays: mitochondrial function assay (Seahorse Agilent technology) and ROS and NO production assays. Created in BioRender. Crisci, E (2025). https://BioRender.com/65x4hji . PRRSV, porcine reproductive and respiratory syndrome virus; MOI, multiplicity of infection; ROS, reactive oxygen species; NO, nitric oxide.
Reference Strain Vr2332, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/reference strain vr2332/product/ATCC
Average 95 stars, based on 1 article reviews
reference strain vr2332 - by Bioz Stars, 2026-03
95/100 stars
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u87392 3 atcc vr 2332 af331831 1 bj 4 af159149 1 mlv respprrs repro lineage 5 eu807840 1 ch 1r ay032626 1 ch 1a ay262352 1  (ATCC)
96
ATCC u87392 3 atcc vr 2332 af331831 1 bj 4 af159149 1 mlv respprrs repro lineage 5 eu807840 1 ch 1r ay032626 1 ch 1a ay262352 1
Experimental layout. Lungs were collected from influenza- and PRRSV-negative 8–12-week-old pigs. Mononuclear phagocytes (MNPs) from bronchoalveolar lavage (BAL) and parenchyma (PAR) were enriched using OptiPrep gradient. MNPs were infected with North Carolina (NC) PRRSV-2 strains NC134 and NC174 (MOI = 1) and control media for 12 h. Infected and control porcine alveolar macrophages (PAMs), pulmonary intravascular macrophages (PIMs), monocyte-derived dendritic cells (moDCs), and classical DCs (cDCs) were sorted, and RNA-seq was performed on each cell subset. Additional PAM and PIM sorted cells were used for qPCR and NanoString validations. For other assays, enriched MNPs were plated to allow macrophage adhesion for 2 h. PAMs and PIMs were subsequently infected with different NC PRRSV-2 strains (NC134, NC174, and NC144) or <t>VR2332</t> prototype strain; uninfected cells were used as negative control. Infected macrophages were used in different assays: mitochondrial function assay (Seahorse Agilent technology) and ROS and NO production assays. Created in BioRender. Crisci, E (2025). https://BioRender.com/65x4hji . PRRSV, porcine reproductive and respiratory syndrome virus; MOI, multiplicity of infection; ROS, reactive oxygen species; NO, nitric oxide.
U87392 3 Atcc Vr 2332 Af331831 1 Bj 4 Af159149 1 Mlv Respprrs Repro Lineage 5 Eu807840 1 Ch 1r Ay032626 1 Ch 1a Ay262352 1, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/u87392 3 atcc vr 2332 af331831 1 bj 4 af159149 1 mlv respprrs repro lineage 5 eu807840 1 ch 1r ay032626 1 ch 1a ay262352 1/product/ATCC
Average 96 stars, based on 1 article reviews
u87392 3 atcc vr 2332 af331831 1 bj 4 af159149 1 mlv respprrs repro lineage 5 eu807840 1 ch 1r ay032626 1 ch 1a ay262352 1 - by Bioz Stars, 2026-03
96/100 stars
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Image Search Results


Experimental layout. Lungs were collected from influenza- and PRRSV-negative 8–12-week-old pigs. Mononuclear phagocytes (MNPs) from bronchoalveolar lavage (BAL) and parenchyma (PAR) were enriched using OptiPrep gradient. MNPs were infected with North Carolina (NC) PRRSV-2 strains NC134 and NC174 (MOI = 1) and control media for 12 h. Infected and control porcine alveolar macrophages (PAMs), pulmonary intravascular macrophages (PIMs), monocyte-derived dendritic cells (moDCs), and classical DCs (cDCs) were sorted, and RNA-seq was performed on each cell subset. Additional PAM and PIM sorted cells were used for qPCR and NanoString validations. For other assays, enriched MNPs were plated to allow macrophage adhesion for 2 h. PAMs and PIMs were subsequently infected with different NC PRRSV-2 strains (NC134, NC174, and NC144) or VR2332 prototype strain; uninfected cells were used as negative control. Infected macrophages were used in different assays: mitochondrial function assay (Seahorse Agilent technology) and ROS and NO production assays. Created in BioRender. Crisci, E (2025). https://BioRender.com/65x4hji . PRRSV, porcine reproductive and respiratory syndrome virus; MOI, multiplicity of infection; ROS, reactive oxygen species; NO, nitric oxide.

Journal: Frontiers in Immunology

Article Title: Mitochondrial dysfunction in PRRSV-2-infected macrophages

doi: 10.3389/fimmu.2025.1670488

Figure Lengend Snippet: Experimental layout. Lungs were collected from influenza- and PRRSV-negative 8–12-week-old pigs. Mononuclear phagocytes (MNPs) from bronchoalveolar lavage (BAL) and parenchyma (PAR) were enriched using OptiPrep gradient. MNPs were infected with North Carolina (NC) PRRSV-2 strains NC134 and NC174 (MOI = 1) and control media for 12 h. Infected and control porcine alveolar macrophages (PAMs), pulmonary intravascular macrophages (PIMs), monocyte-derived dendritic cells (moDCs), and classical DCs (cDCs) were sorted, and RNA-seq was performed on each cell subset. Additional PAM and PIM sorted cells were used for qPCR and NanoString validations. For other assays, enriched MNPs were plated to allow macrophage adhesion for 2 h. PAMs and PIMs were subsequently infected with different NC PRRSV-2 strains (NC134, NC174, and NC144) or VR2332 prototype strain; uninfected cells were used as negative control. Infected macrophages were used in different assays: mitochondrial function assay (Seahorse Agilent technology) and ROS and NO production assays. Created in BioRender. Crisci, E (2025). https://BioRender.com/65x4hji . PRRSV, porcine reproductive and respiratory syndrome virus; MOI, multiplicity of infection; ROS, reactive oxygen species; NO, nitric oxide.

Article Snippet: Additionally, the reference strain VR2332 (ATCC strain BIAH-001, GenBank accession ID U87392.3 , L5A.1) was used as the PRRSV-2 prototype strain.

Techniques: Infection, Control, Derivative Assay, RNA Sequencing, Negative Control, Functional Assay, Virus