u6 Search Results


shrna sequence  (Vector Biolabs)
95
Vector Biolabs shrna sequence
Nrxn3 <t>shRNA</t> reduced Nrxn3 expression The central amygdala was infused with adeno-associated virus containing either a control <t>(scrambled</t> <t>sequence)</t> shRNA or a Nrxn3 shRNA expression construct. After four weeks the masseter tendon was ligated bilaterally, or a sham surgery was performed and the animals sacrificed three weeks after ligature. Central amygdala tissue was isolated by biopsy punches of frozen sections and the quantity of Nrxn3 was quantitated by ELISA. Significant differences of (P < 0.05) between the groups are indicated by “*”. Each point on the graph is from a different animal. Values are the means ± SEM for 8 animals per treatment group.
Shrna Sequence, supplied by Vector Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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feng zhang  (Addgene inc)
95
Addgene inc feng zhang
Nrxn3 <t>shRNA</t> reduced Nrxn3 expression The central amygdala was infused with adeno-associated virus containing either a control <t>(scrambled</t> <t>sequence)</t> shRNA or a Nrxn3 shRNA expression construct. After four weeks the masseter tendon was ligated bilaterally, or a sham surgery was performed and the animals sacrificed three weeks after ligature. Central amygdala tissue was isolated by biopsy punches of frozen sections and the quantity of Nrxn3 was quantitated by ELISA. Significant differences of (P < 0.05) between the groups are indicated by “*”. Each point on the graph is from a different animal. Values are the means ± SEM for 8 animals per treatment group.
Feng Zhang, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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px330 u6 chimeric bbcbh hspcas9 plasmid  (Addgene inc)
96
Addgene inc px330 u6 chimeric bbcbh hspcas9 plasmid
Nrxn3 <t>shRNA</t> reduced Nrxn3 expression The central amygdala was infused with adeno-associated virus containing either a control <t>(scrambled</t> <t>sequence)</t> shRNA or a Nrxn3 shRNA expression construct. After four weeks the masseter tendon was ligated bilaterally, or a sham surgery was performed and the animals sacrificed three weeks after ligature. Central amygdala tissue was isolated by biopsy punches of frozen sections and the quantity of Nrxn3 was quantitated by ELISA. Significant differences of (P < 0.05) between the groups are indicated by “*”. Each point on the graph is from a different animal. Values are the means ± SEM for 8 animals per treatment group.
Px330 U6 Chimeric Bbcbh Hspcas9 Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pslq2853 3 phr  (Addgene inc)
93
Addgene inc pslq2853 3 phr
Nrxn3 <t>shRNA</t> reduced Nrxn3 expression The central amygdala was infused with adeno-associated virus containing either a control <t>(scrambled</t> <t>sequence)</t> shRNA or a Nrxn3 shRNA expression construct. After four weeks the masseter tendon was ligated bilaterally, or a sham surgery was performed and the animals sacrificed three weeks after ligature. Central amygdala tissue was isolated by biopsy punches of frozen sections and the quantity of Nrxn3 was quantitated by ELISA. Significant differences of (P < 0.05) between the groups are indicated by “*”. Each point on the graph is from a different animal. Values are the means ± SEM for 8 animals per treatment group.
Pslq2853 3 Phr, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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u6 sguprt  (Addgene inc)
93
Addgene inc u6 sguprt
Nrxn3 <t>shRNA</t> reduced Nrxn3 expression The central amygdala was infused with adeno-associated virus containing either a control <t>(scrambled</t> <t>sequence)</t> shRNA or a Nrxn3 shRNA expression construct. After four weeks the masseter tendon was ligated bilaterally, or a sham surgery was performed and the animals sacrificed three weeks after ligature. Central amygdala tissue was isolated by biopsy punches of frozen sections and the quantity of Nrxn3 was quantitated by ELISA. Significant differences of (P < 0.05) between the groups are indicated by “*”. Each point on the graph is from a different animal. Values are the means ± SEM for 8 animals per treatment group.
U6 Sguprt, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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px335  (Addgene inc)
96
Addgene inc px335
Nrxn3 <t>shRNA</t> reduced Nrxn3 expression The central amygdala was infused with adeno-associated virus containing either a control <t>(scrambled</t> <t>sequence)</t> shRNA or a Nrxn3 shRNA expression construct. After four weeks the masseter tendon was ligated bilaterally, or a sham surgery was performed and the animals sacrificed three weeks after ligature. Central amygdala tissue was isolated by biopsy punches of frozen sections and the quantity of Nrxn3 was quantitated by ELISA. Significant differences of (P < 0.05) between the groups are indicated by “*”. Each point on the graph is from a different animal. Values are the means ± SEM for 8 animals per treatment group.
Px335, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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plasmid 61591  (Addgene inc)
94
Addgene inc plasmid 61591
Nrxn3 <t>shRNA</t> reduced Nrxn3 expression The central amygdala was infused with adeno-associated virus containing either a control <t>(scrambled</t> <t>sequence)</t> shRNA or a Nrxn3 shRNA expression construct. After four weeks the masseter tendon was ligated bilaterally, or a sham surgery was performed and the animals sacrificed three weeks after ligature. Central amygdala tissue was isolated by biopsy punches of frozen sections and the quantity of Nrxn3 was quantitated by ELISA. Significant differences of (P < 0.05) between the groups are indicated by “*”. Each point on the graph is from a different animal. Values are the means ± SEM for 8 animals per treatment group.
Plasmid 61591, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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plasmid pgl3 u6 sgrna pgk puromycin  (Addgene inc)
94
Addgene inc plasmid pgl3 u6 sgrna pgk puromycin
Nrxn3 <t>shRNA</t> reduced Nrxn3 expression The central amygdala was infused with adeno-associated virus containing either a control <t>(scrambled</t> <t>sequence)</t> shRNA or a Nrxn3 shRNA expression construct. After four weeks the masseter tendon was ligated bilaterally, or a sham surgery was performed and the animals sacrificed three weeks after ligature. Central amygdala tissue was isolated by biopsy punches of frozen sections and the quantity of Nrxn3 was quantitated by ELISA. Significant differences of (P < 0.05) between the groups are indicated by “*”. Each point on the graph is from a different animal. Values are the means ± SEM for 8 animals per treatment group.
Plasmid Pgl3 U6 Sgrna Pgk Puromycin, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pdesttol2pa2 u6 grna  (Addgene inc)
92
Addgene inc pdesttol2pa2 u6 grna
Nrxn3 <t>shRNA</t> reduced Nrxn3 expression The central amygdala was infused with adeno-associated virus containing either a control <t>(scrambled</t> <t>sequence)</t> shRNA or a Nrxn3 shRNA expression construct. After four weeks the masseter tendon was ligated bilaterally, or a sham surgery was performed and the animals sacrificed three weeks after ligature. Central amygdala tissue was isolated by biopsy punches of frozen sections and the quantity of Nrxn3 was quantitated by ELISA. Significant differences of (P < 0.05) between the groups are indicated by “*”. Each point on the graph is from a different animal. Values are the means ± SEM for 8 animals per treatment group.
Pdesttol2pa2 U6 Grna, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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px260  (Addgene inc)
93
Addgene inc px260
Nrxn3 <t>shRNA</t> reduced Nrxn3 expression The central amygdala was infused with adeno-associated virus containing either a control <t>(scrambled</t> <t>sequence)</t> shRNA or a Nrxn3 shRNA expression construct. After four weeks the masseter tendon was ligated bilaterally, or a sham surgery was performed and the animals sacrificed three weeks after ligature. Central amygdala tissue was isolated by biopsy punches of frozen sections and the quantity of Nrxn3 was quantitated by ELISA. Significant differences of (P < 0.05) between the groups are indicated by “*”. Each point on the graph is from a different animal. Values are the means ± SEM for 8 animals per treatment group.
Px260, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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phage tre dcas9 krab lentiviral plasmid31 encoding dcas9 krab  (Addgene inc)
93
Addgene inc phage tre dcas9 krab lentiviral plasmid31 encoding dcas9 krab
Nrxn3 <t>shRNA</t> reduced Nrxn3 expression The central amygdala was infused with adeno-associated virus containing either a control <t>(scrambled</t> <t>sequence)</t> shRNA or a Nrxn3 shRNA expression construct. After four weeks the masseter tendon was ligated bilaterally, or a sham surgery was performed and the animals sacrificed three weeks after ligature. Central amygdala tissue was isolated by biopsy punches of frozen sections and the quantity of Nrxn3 was quantitated by ELISA. Significant differences of (P < 0.05) between the groups are indicated by “*”. Each point on the graph is from a different animal. Values are the means ± SEM for 8 animals per treatment group.
Phage Tre Dcas9 Krab Lentiviral Plasmid31 Encoding Dcas9 Krab, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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addgene plasmid 60229  (Addgene inc)
94
Addgene inc addgene plasmid 60229
Nrxn3 <t>shRNA</t> reduced Nrxn3 expression The central amygdala was infused with adeno-associated virus containing either a control <t>(scrambled</t> <t>sequence)</t> shRNA or a Nrxn3 shRNA expression construct. After four weeks the masseter tendon was ligated bilaterally, or a sham surgery was performed and the animals sacrificed three weeks after ligature. Central amygdala tissue was isolated by biopsy punches of frozen sections and the quantity of Nrxn3 was quantitated by ELISA. Significant differences of (P < 0.05) between the groups are indicated by “*”. Each point on the graph is from a different animal. Values are the means ± SEM for 8 animals per treatment group.
Addgene Plasmid 60229, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Nrxn3 shRNA reduced Nrxn3 expression The central amygdala was infused with adeno-associated virus containing either a control (scrambled sequence) shRNA or a Nrxn3 shRNA expression construct. After four weeks the masseter tendon was ligated bilaterally, or a sham surgery was performed and the animals sacrificed three weeks after ligature. Central amygdala tissue was isolated by biopsy punches of frozen sections and the quantity of Nrxn3 was quantitated by ELISA. Significant differences of (P < 0.05) between the groups are indicated by “*”. Each point on the graph is from a different animal. Values are the means ± SEM for 8 animals per treatment group.

Journal: Neurobiology of Pain

Article Title: Nrxn3 reduces myofascial nociceptive pain

doi: 10.1016/j.ynpai.2025.100197

Figure Lengend Snippet: Nrxn3 shRNA reduced Nrxn3 expression The central amygdala was infused with adeno-associated virus containing either a control (scrambled sequence) shRNA or a Nrxn3 shRNA expression construct. After four weeks the masseter tendon was ligated bilaterally, or a sham surgery was performed and the animals sacrificed three weeks after ligature. Central amygdala tissue was isolated by biopsy punches of frozen sections and the quantity of Nrxn3 was quantitated by ELISA. Significant differences of (P < 0.05) between the groups are indicated by “*”. Each point on the graph is from a different animal. Values are the means ± SEM for 8 animals per treatment group.

Article Snippet: A separate group of rats was infused with 1 μl of 1X10 13 TU/mL AAV1 containing a scrambled shRNA sequence (AAV1-GFP-U6-shRNA, Vector Biolabs).

Techniques: shRNA, Expressing, Virus, Control, Sequencing, Construct, Isolation, Enzyme-linked Immunosorbent Assay