Journal: Nucleic Acids Research

Article Title: An Lnc RNA (GAS5)/SnoRNA-derived piRNA induces activation of TRAIL gene by site-specifically recruiting MLL/COMPASS-like complexes

doi: 10.1093/nar/gkv214

Figure Lengend Snippet: Gas5-derived small RNA (pi-sno75) specifically upregulates TRAIL expression in breast cancer cells. ( A ) The mRNA profiling microarray analysis. The gene expression scatterplot of the piRNAs mixture group versus that of the control group was shown. Significantly downregulated genes were shown in green, while upregulated genes in red. ( B ) MCF7 cells were transfected with piRNAs derived from GAS5 respectively for 48h, TRAIL mRNA levels were analysed by quantitative RT-PCR (qRT-PCR) and normalized to GAPDH levels. ( C ) MCF7 cells were transfected with pi-sno75 at different concentrations. The qRT-PCR was performed to analyse the effect of pi-sno75 on TRAIL expression. ( D ) MCF7 cells were transfected with pi-sno75 and piNC for 48h, western blot was performed to analyse TRAIL protein expression. ( E ) In MCF7 cells transfected with pi-sno75, the mRNA levels of TRAIL and other genes were determined by qRT-PCR. Bar graphs in (B), (C) and (E) represent mean ± SD ( n = 3).

Article Snippet: Gene expression profiles were analysed with the Whole Human Genome Microarray (Human Genome U133 Plus 2.0, Affymetrix).

Techniques: Derivative Assay, Expressing, Microarray, Transfection, Quantitative RT-PCR, Western Blot

Journal: International Journal of Molecular Sciences

Article Title: Biomarkers of Periodontitis and Its Differential DNA Methylation and Gene Expression in Immune Cells: A Systematic Review

doi: 10.3390/ijms231912042

Figure Lengend Snippet: Studies on mRNA expression in peripheral blood mononuclear cells (PBMCs) or subcomponents (lymphocytes and monocytes).

Article Snippet: Corbi S.C.T. et al., 2020 [ ] , 24 periodontitis patients for Microarray expression (U133 Plus 2.0, Affimetrix): • 5 poorly controlled T2DM + dyslipidemia + periodontitis (T2DMpoorly-DL-P) • 7 well-controlled T2DM with dyslipidemia and periodontitis (T2DMwell-DL-P) • 6 well-controlled T2DM + dyslipidemia + periodontitis (DL-P) • 6 normoglycemic individuals + dyslipidemia + periodontitis (P) 120 periodontitis patients for RT-qPCR validation of selected DEGs: • 30 T2DMpoorly-DL-P • 30 T2DMwell-DL-P • 30 DL-P • 30 P , For Microarray U133 Plus 2.0: • 6 systemically healthy individuals without periodontitis (H) (homogeneity regarding biochemical, lipid and clinical periodontal parameters). For RT-qPCR validation: - 30 H , Mono-nuclear cells (Lymphocytes and monocytes)/peripheral blood , Expression Microarray U133 Plus 2.0 RT-qPCR (validation) , • P ↓ mRNA IGHG3 ↑ mRNA ITGB2 and HLADRB4. • T2DMpoorly + DL + P ↑ mRNA TGFB1I1 , VNN1 ↓ mRNA HLADRB4 and CXCL8 • T2Dmwell + DL + P ↓ mRNA BPTF , PDE3B ↑ mRNA FN1 • DL + P ↑ mRNA DAB2 ↓ mRNA CD47 and HLADRB4.

Techniques: Expressing, Microarray, Centrifugation, Real-time Polymerase Chain Reaction, Reverse Transcription Polymerase Chain Reaction, Isolation