Journal: Oncogene

Article Title: β-catenin S45F mutation results in apoptotic resistance

doi: 10.1038/s41388-020-1382-5

Figure Lengend Snippet: a Effects of staurosporine and b doxorubicin on cell apoptosis were measured by flow cytometry. Representative cleaved-caspase 3/7 fluorescent dye images of 2 desmoid cell strains. Effects of c staurosporine and d doxorubicin on cell caspase-dependent apoptosis were measured using automated IncuCyte imaging. DMSO, dimethyl sulfoxide. Error bars represent the standard deviation for three independent experiments. ** P < 0.001.

Article Snippet: Data were analyzed using Incucyte software (Essen Biosciences).

Techniques: Flow Cytometry, Imaging, Standard Deviation

Journal: Oncogene

Article Title: β-catenin S45F mutation results in apoptotic resistance

doi: 10.1038/s41388-020-1382-5

Figure Lengend Snippet: a Effects of staurosporine and b doxorubicin on cell apoptosis were measured by flow cytometry. Representative cleaved-caspase 3/7 fluorescent dye images of transfected 293T cells and controls. Effects of c staurosporine and d doxorubicin on cell caspase-dependent apoptosis were measured using automated IncuCyte imaging. DMSO, dimethyl sulfoxide. Error bars represent the standard deviation for three independent experiments. ** P < 0.001.

Article Snippet: Data were analyzed using Incucyte software (Essen Biosciences).

Techniques: Flow Cytometry, Transfection, Imaging, Standard Deviation

Journal: Oncogene

Article Title: β-catenin S45F mutation results in apoptotic resistance

doi: 10.1038/s41388-020-1382-5

Figure Lengend Snippet: a Effects of doxorubicin on cell apoptosis were measured by way of flow cytometry. b Representative cleaved-caspase 3/7 fluorescent dye images of transfected 293T cells and controls. Effects of doxorubicin on cell caspase-dependent apoptosis were measured using automated IncuCyte imaging. DMSO, dimethyl sulfoxide. Error bars represent the standard deviation for three independent experiments. ** P < 0.001.

Article Snippet: Data were analyzed using Incucyte software (Essen Biosciences).

Techniques: Flow Cytometry, Transfection, Imaging, Standard Deviation

Journal: Oncology Letters

Article Title: MicroRNA-545-5p regulates apoptosis, migration and invasion of osteosarcoma by targeting dimethyladenosine transferase 1

doi: 10.3892/ol.2021.13024

Figure Lengend Snippet: miR-545-5p facilitates osteosarcoma cell apoptosis. (A) 2×10 3 MG63 or Saos-2 cells were transfected with miR-545-5p mimic or mimic NC for 48 h and proliferating cells were labeled with EdU. Nuclei were labeled with DAPI (blue) and EdU-positive cells are highlighted in green. The number of proliferating cells were quantified using ImageJ software version 1.49. Cells without transfection were also analyzed (control). (Scale bar, 50 µm and magnification, ×100). (B) The Cell Counting Kit-8 assay was performed to detect the viability of Saos-2 and MG63 cells transfected with miR-545-5p mimic of mimic NC for 48 h. Cells without transfection were also analyzed (control). (C) 3×10 5 Saos-2 or MG63 cells transfected with 50 nM mimic NC or miR-545-5p mimic for 48 h were harvested and labeled with Annexin V-FITC and PI. Samples were assessed via flow cytometry. The numbers in each quadrant indicate positive percentages of the entire population. *P<0.05 vs. control. miR, microRNA; NC, negative control; PI, propidium iodide.

Article Snippet: Cell apoptosis was analyzed using the Annexin V-FITC Apoptosis kit (cat. no. K101; Biovision, Inc.), according to the manufacturer's instructions.

Techniques: Transfection, Labeling, Software, Control, Cell Counting, Flow Cytometry, Negative Control

Journal: Oncology Letters

Article Title: MicroRNA-545-5p regulates apoptosis, migration and invasion of osteosarcoma by targeting dimethyladenosine transferase 1

doi: 10.3892/ol.2021.13024

Figure Lengend Snippet: miR-545-5p functions by targeting DIMT1. (A) Saos-2 and MG63 cells were transfected with 50 nM miR-545-5p mimic or mimic NC, with or without co-transfection of 10 µg DIMT1 overexpression plasmid for 48 h and western blot analysis was performed to detect DIMT1 protein expression. GAPDH was used as the loading control. (B) Saos-2 and MG63 cells were transfected with 50 nM miR-545-5p mimic or mimic NC, with or without co-transfection of 10 µg DIMT1 overexpression plasmid for 48 h and the Cell Counting Kit-8 assay was performed to detect cell viability. (C) Saos-2 and MG63 cells were transfected with 50 nM miR-545-5p mimic or mimic NC, with or without co-transfection of 10 µg DIMT1 overexpression plasmid for 24 h and the Transwell assay was performed to detect cell invasion. Quantification analysis is depicted in the lower panel. Scale bar, 50 µm and magnification, ×100. (D) Flow cytometric analysis of Saos-2 and MG63 cells transfected with 50 nM miR-545-5p mimic or mimic NC, with or without co-transfection of 10 µg DIMT1 overexpression plasmid for 48 h. Cells were harvested and labeled with Annexin V-FITC and PI. The numbers in each quadrant indicate positive percentages of the entire population. (E) Saos-2 and MG63 cells were transfected with 50 µm miR-545-5p mimic or mimic NC, with or without co-transfection of 10 µg DIMT1 overexpression plasmid and the wound healing assay was performed to detect cell migration. Quantification analysis is depicted in the right panel. Scale bar, 50 µm. *P<0.05 vs. mimic NC. # P<0.05 vs. miR-545-5p mimic. miR, microRNA; DIMT1, dimethyladenosine transferase 1; NC, negative control; PI, propidium iodide.

Article Snippet: Cell apoptosis was analyzed using the Annexin V-FITC Apoptosis kit (cat. no. K101; Biovision, Inc.), according to the manufacturer's instructions.

Techniques: Transfection, Cotransfection, Over Expression, Plasmid Preparation, Western Blot, Expressing, Control, Cell Counting, Transwell Assay, Labeling, Wound Healing Assay, Migration, Negative Control

Journal: Cell reports

Article Title: Poxvirus A51R proteins regulate microtubule stability and antagonize a cell-intrinsic antiviral response

doi: 10.1016/j.celrep.2024.113882

Figure Lengend Snippet:

Article Snippet: Cercopithecus aethiops: BSC-40 kidney epithelial cells (female) , ATCC , CRL-2761.

Techniques: Virus, Control, Recombinant, Lysis, Staining, Protease Inhibitor, Magnetic Beads, Modification, Saline, Expressing, Transfection, Blocking Assay, Fractionation, HTS Assay, Plasmid Preparation, Software, Imaging

Journal: iScience

Article Title: RGD targeted magnetic ferrite nanoparticles enhance antitumor immunotherapeutic efficacy by activating STING signaling pathway

doi: 10.1016/j.isci.2024.109062

Figure Lengend Snippet: Preparation and characterization of RGD-NP (A) Synthesis procedure of RGD-NP. (B) TEM images of RGD-NP. (C) Surface charges (zeta-potential) of RGD-NP in specific solution. (D) Hydrodynamic diameter of RGD-NP in water during 19 days. Data are showed in mean ± SD. (E) Hydrodynamic diameter of RGD-NP in different solution on the day 19th. (F and G) Absorption spectrum and emission fluorescence spectrum of Cy5.5-RGD-NP and other control nanoparticles. (H) Immunofluorescence imaging of CT26-GFP tumor cells treated with Cy5.5-RGD-NP and Cy5.5-NP, respectively Scale bar, 50 μm. (I) Flow cytometry of CT26-GFP cells treated with different nanoparticles. Data are represented as mean ± SEM. (ns: p > 0.05, ∗: p < 0.05, ∗∗: p < 0.01, ∗∗∗: p < 0.001, ∗∗∗∗: p < 0.001).

Article Snippet: Mouse CT26 cells , ATCC , Cat# CRL-2638.

Techniques: Zeta Potential Analyzer, Fluorescence, Control, Immunofluorescence, Imaging, Flow Cytometry

Journal: iScience

Article Title: RGD targeted magnetic ferrite nanoparticles enhance antitumor immunotherapeutic efficacy by activating STING signaling pathway

doi: 10.1016/j.isci.2024.109062

Figure Lengend Snippet: Specific tumor targeted FMI/MRI imaging of RGD-NPs in both in vivo and ex vivo CT26 tumor bearing mice (A) FMI of CT26 tumor-bearing mice treated with Cy5.5-NP or Cy5.5-RGD-NP at different time points. (B) The signal-to-background ratio (SBR) of in vivo FMI results. (C) Ex vivo FMI of tumors and internal organs at 48 h post-injection of nanoparticles. (D) The T1-weighted MRI of CT26 tumors at different time points. Data are represented as mean ± SEM. (ns: p > 0.05, ∗: p < 0.05, ∗∗: p < 0.01, ∗∗∗: p < 0.001, ∗∗∗∗: p < 0.001).

Article Snippet: Mouse CT26 cells , ATCC , Cat# CRL-2638.

Techniques: Imaging, In Vivo, Ex Vivo, Injection

Journal: iScience

Article Title: RGD targeted magnetic ferrite nanoparticles enhance antitumor immunotherapeutic efficacy by activating STING signaling pathway

doi: 10.1016/j.isci.2024.109062

Figure Lengend Snippet: Antitumor effects of combined RGD-NP and aPD-L1 immunotherapy (A‒C) Tumor volume was monitored dynamically with different treatments on B16F10, CT26, and 4T1 tumors. (n = 6–10). (D‒F) The mouse body weight in each group was measured dynamically during the treatment period. (G and H) Quantification of tumor weights and representative images of tumors at the end of CT26 treatment. Data are represented as mean ± SEM. (ns: p > 0.05, ∗: p < 0.05, ∗∗: p < 0.01, ∗∗∗: p < 0.001, ∗∗∗∗: p < 0.001).

Article Snippet: Mouse CT26 cells , ATCC , Cat# CRL-2638.

Techniques:

Journal: iScience

Article Title: RGD targeted magnetic ferrite nanoparticles enhance antitumor immunotherapeutic efficacy by activating STING signaling pathway

doi: 10.1016/j.isci.2024.109062

Figure Lengend Snippet: RGD-NP promote the recruitment of tumor-infiltrating cytotoxic T lymphocytes (CTLs) (A‒E) Flow cytometry results of tumors harvested to analyze tumor-infiltrating leukocytes with different treatments. (F and G) Tumors and lymph nodes were lysed to obtain protein samples. Relative amounts of p -TBK1, total TBK1, and GAPDH were measured via western blot. (H) Immunohistochemical staining of CD8 + T cells in CT26 tumors. Scale bar, 50 μm Data are represented as mean ± SEM. (ns: p > 0.05, ∗: p < 0.05, ∗∗: p < 0.01, ∗∗∗: p < 0.001, ∗∗∗∗: p < 0.001).

Article Snippet: Mouse CT26 cells , ATCC , Cat# CRL-2638.

Techniques: Flow Cytometry, Western Blot, Immunohistochemical staining, Staining

Journal: iScience

Article Title: RGD targeted magnetic ferrite nanoparticles enhance antitumor immunotherapeutic efficacy by activating STING signaling pathway

doi: 10.1016/j.isci.2024.109062

Figure Lengend Snippet:

Article Snippet: Mouse CT26 cells , ATCC , Cat# CRL-2638.

Techniques: Recombinant, Red Blood Cell Lysis, Software, Imaging