Journal: eLife

Article Title: Dendritic cells loaded with FK506 kill T cells in an antigen-specific manner and prevent autoimmunity in vivo

doi: 10.7554/eLife.00105

Figure Lengend Snippet: ( A ) DBA1/J bone marrow derived DC pulsed with either type I or II collagen and treated with FK506 or media. DC were transferred intravenously 7 and 14 days after type II collagen/CFA immunization. Data are mean arthritis severity score of each group on each day. N = 8–12 per group. ( B ) Incidence of severe arthritis. ( C ) Histology of normal paw and paw of CIA mouse treated with FKDC-CII or DC-CII (mouse with median histologic score per group is presented). C: Cartilage damage; R: Bone resorption; I: inflammation; P: pannus; O: Osteophyte. ( D ) Radiographs from paws presented in ( C ). ( E ) CD4+ splenocytes from an untreated mouse were cultured with serum drawn from PBS, FK506 or FKDC treated mice after the last dose of treatment (day 14), and the CD4+ T cells then stimulated for 4 hr with CD3/28 beads. Data are mean fold induction of IL2 mRNA of stimulated vs unstimulated groups + standard deviation. N = 6–10 mice per group. p value was calculated using a two tailed t-test to compare FK506 and FKDC groups. ( F ) Serum chemistries of mice treated with 14 days, as indicated, with PBS, FK506 or FKDC, as described in ( E ). N = 6–10 mice per group. Gray circles are serum amylase values for individual mice. Black bars represent median values per group. p value was calculated using a two-tailed Mann–Whitney test comparing FK506 and FKDC treated groups. Data from ( E ) and ( F ) are representative of two independent experiments. DOI: http://dx.doi.org/10.7554/eLife.00105.007

Article Snippet: Bone marrow derived DC ( ) pulsed with 50 μg/ml bovine type II collagen or bovine type I collagen (Chondrex) were subsequently treated with media or 0.5 μM FK506 for 2 hr and washed.

Techniques: Derivative Assay, Cell Culture, Standard Deviation, Two Tailed Test, MANN-WHITNEY

Journal: The Journal of Infectious Diseases

Article Title: Molecular Diagnostic Field Test for Point-of-Care Detection of Ebola Virus Directly From Blood

doi: 10.1093/infdis/jiw330

Figure Lengend Snippet: List of the Pathogens Used for the Evaluation of the Specificity of Ebola Virus (EBOV) Reverse Transcription–Loop-Mediated Isothermal Amplification (RT-LAMP)

Article Snippet: Final reaction products were visualized by electrophoresis on 2% agarose gels stained with ethidium bromide. table ft1 table-wrap mode="anchored" t5 Table 2. caption a7 Sample Isolate Source Part Number Stock Titer Titer/Test (50 µL) 1 a Marburg virus–Angola UTMB Not available 8 × 10 7 PFU/mL 2 × 10 4 PFU/mL 2 a Marburg virus–Musoke UTMB Not available 4.4 × 10 6 PFU/mL 1.1 × 10 3 PFU/mL 3 a Lassa virus UTMB Not available 1.75 × 10 6 PFU/mL 4.25 × 10 2 PFU/mL 4 a Crimean-Congo hemorrhagic fever virus UTMB Not available 1 × 10 7 PFU/mL 2.5 × 10 3 PFU/mL 5 Respiratory syncytial virus A (2006 isolate) Zeptometrix 0810040ACF 1.95 × 10 7 U/mL TCID 50 5.36 × 10 3 units 6 Respiratory syncytial virus B (CH93(18)-18) Zeptometrix 0810040CF 1.51 × 10 6 U/mL TCID 50 4.16 × 10 2 units 7 Influenza A virus (Wisconsin/67/05) Zeptometrix 0810252CF 1.41 × 10 5 U/mL TCID 50 3.88 × 10 1 units 8 Influenza B virus (Massachusetts/2/12) Zeptometrix 0810239CF 1.41 × 10 5 U/mL TCID 50 3.88 × 10 1 units 9 Salmonella enterica subspecies arizonae ATCC d ATCC 13314 1.30 × 10 8 CFU/vial (1 mL) 3.58 × 10 4 CFU 10 Salmonella enterica serotype Typhi Zeptometrix 0801933 1.89 × 10 9 CFU/mL 5.20 × 10 5 CFU 11 Pseudomonas aeruginosa ATCC ATCC 10145 9.15 × 10 7 CFU/mL 2.52 × 10 4 CFU 12 Salmonella sonnei ATCC ATCC 29930 >10 4 CFU/mL 2.75 × 10 1 CFU 13 Plasmodium vivax ATCC ATCC 30152 4.90 × 10 6 U/mL TCID 50 1.35 × 10 3 units 14 Plasmodium falciparum ATCC ATCC 30932 2.84% parasitemia/vial (0.7 mL) 0.02% parasitemia 15 Dengue virus serotype 1 (Hawaii) Zeptometrix 0810088CF 2.45 × 10 5 U/mL TCID 50 6.74 × 10 1 units 16 Dengue virus serotype 2 (New Guinea) Zeptometrix 0810089CF 3.16 × 10 5 U/mL TCID 50 8.69 × 10 1 units 17 Dengue virus serotype 3 (strain H87) Zeptometrix 0810090CF 1.15 × 10 7 U/mL TCID 50 3.16 × 10 3 units 18 Dengue virus serotype 4 (H241) Zeptometrix 0810091CF 3.39 × 10 7 U/mL TCID 50 9.32 × 10 3 units 19 Vibrio cholerae (Z133) Zeptometrix 0801902 1.90 × 10 10 CFU/mL 5.23 × 10 6 CFU Open in a separate window Abbreviations: ATTC, American Type Culture Collection; CFU, colony-forming units; PFU, plaque-forming units; TCID 50 , 50% tissue culture infective dose; UTMB, University of Texas Medical Branch, Galveston. a Specificity experiments using these pathogens were done at the UTMB in a biosafety level 4 facility.

Techniques: Virus, Amplification

Journal: The Journal of Infectious Diseases

Article Title: Molecular Diagnostic Field Test for Point-of-Care Detection of Ebola Virus Directly From Blood

doi: 10.1093/infdis/jiw330

Figure Lengend Snippet: List of the Pathogens Used for the Evaluation of the Specificity of Ebola Virus (EBOV) Reverse Transcription–Loop-Mediated Isothermal Amplification (RT-LAMP)

Article Snippet: Final reaction products were visualized by electrophoresis on 2% agarose gels stained with ethidium bromide. table ft1 table-wrap mode="anchored" t5 Table 2. caption a7 Sample Isolate Source Part Number Stock Titer Titer/Test (50 µL) 1 a Marburg virus–Angola UTMB Not available 8 × 10 7 PFU/mL 2 × 10 4 PFU/mL 2 a Marburg virus–Musoke UTMB Not available 4.4 × 10 6 PFU/mL 1.1 × 10 3 PFU/mL 3 a Lassa virus UTMB Not available 1.75 × 10 6 PFU/mL 4.25 × 10 2 PFU/mL 4 a Crimean-Congo hemorrhagic fever virus UTMB Not available 1 × 10 7 PFU/mL 2.5 × 10 3 PFU/mL 5 Respiratory syncytial virus A (2006 isolate) Zeptometrix 0810040ACF 1.95 × 10 7 U/mL TCID 50 5.36 × 10 3 units 6 Respiratory syncytial virus B (CH93(18)-18) Zeptometrix 0810040CF 1.51 × 10 6 U/mL TCID 50 4.16 × 10 2 units 7 Influenza A virus (Wisconsin/67/05) Zeptometrix 0810252CF 1.41 × 10 5 U/mL TCID 50 3.88 × 10 1 units 8 Influenza B virus (Massachusetts/2/12) Zeptometrix 0810239CF 1.41 × 10 5 U/mL TCID 50 3.88 × 10 1 units 9 Salmonella enterica subspecies arizonae ATCC d ATCC 13314 1.30 × 10 8 CFU/vial (1 mL) 3.58 × 10 4 CFU 10 Salmonella enterica serotype Typhi Zeptometrix 0801933 1.89 × 10 9 CFU/mL 5.20 × 10 5 CFU 11 Pseudomonas aeruginosa ATCC ATCC 10145 9.15 × 10 7 CFU/mL 2.52 × 10 4 CFU 12 Salmonella sonnei ATCC ATCC 29930 >10 4 CFU/mL 2.75 × 10 1 CFU 13 Plasmodium vivax ATCC ATCC 30152 4.90 × 10 6 U/mL TCID 50 1.35 × 10 3 units 14 Plasmodium falciparum ATCC ATCC 30932 2.84% parasitemia/vial (0.7 mL) 0.02% parasitemia 15 Dengue virus serotype 1 (Hawaii) Zeptometrix 0810088CF 2.45 × 10 5 U/mL TCID 50 6.74 × 10 1 units 16 Dengue virus serotype 2 (New Guinea) Zeptometrix 0810089CF 3.16 × 10 5 U/mL TCID 50 8.69 × 10 1 units 17 Dengue virus serotype 3 (strain H87) Zeptometrix 0810090CF 1.15 × 10 7 U/mL TCID 50 3.16 × 10 3 units 18 Dengue virus serotype 4 (H241) Zeptometrix 0810091CF 3.39 × 10 7 U/mL TCID 50 9.32 × 10 3 units 19 Vibrio cholerae (Z133) Zeptometrix 0801902 1.90 × 10 10 CFU/mL 5.23 × 10 6 CFU Open in a separate window Abbreviations: ATTC, American Type Culture Collection; CFU, colony-forming units; PFU, plaque-forming units; TCID 50 , 50% tissue culture infective dose; UTMB, University of Texas Medical Branch, Galveston. a Specificity experiments using these pathogens were done at the UTMB in a biosafety level 4 facility.

Techniques: Virus, Amplification