Journal: iScience

Article Title: Mof plays distinct roles in hepatic lipid metabolism under healthy or non-alcoholic fatty liver conditions

doi: 10.1016/j.isci.2023.108446

Figure Lengend Snippet: Mof played distinct roles in lipid metabolism via PPARα or mTOR signaling pathway under different circumstances (A) AML 12 cells were subjected to Oil-O staining following treatment with either DMSO or mg149. Scale bar: 50 μm. See also Figure S4 C. (B) Oil-O staining was performed on AML 12 cells treated with PA + DMSO or PA + mg149. Scale bar: 50 μm. See also Figure S4 D. (C) The effect of mg149 on H4K16ac, PPARɑ, LC3, and mTOR was assessed using western blotting (n = 3). GAPDH was the loading control, with quantification in the right panel. (D) Western blotting analysis revealed the influence of PA on Mof, H4K16ac, p -mTOR, mTOR, and PPARɑ (n = 3). GAPDH was the loading control, with quantification in the right panel. (E) Western blotting demonstrated the impact of mg149 on mTOR in AML 12 cells treated with PA (n = 3). GAPDH was the loading control, with quantification in the right panel. Graph data were presented as mean ± SEM. n represents the number of western blotting data. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001 (Student’s t test).

Article Snippet: From 8 weeks, one group was given 1 mg/kg mg149 (CAS:1243583-85-8, Sellck, China) dissolved in DMSO, Tween 80 (CAS:9005-65-6, Sellck), and PEG 300 (CAS: 25322-68-3, Selleck), intraperitoneally every other day, while the other was given DMSO solvent as control.

Techniques: Staining, Western Blot, Control

Journal: iScience

Article Title: Mof plays distinct roles in hepatic lipid metabolism under healthy or non-alcoholic fatty liver conditions

doi: 10.1016/j.isci.2023.108446

Figure Lengend Snippet: Impact of Mof inhibition on wild-type hepatic metabolism (A and B) IPGTT and IPITT of untreated WT mice (n = 7). (C and D) IPGTT and IPITT of DMSO or mg149 administrated WT mice (n = 7). (E) Western blotting showed that mg149 treatment led to decreased hepatic PPARα and H4K16ac expressions (n = 4). (F) Measurements of serum TG levels of WT + DMSO and WT + mg149 groups (n = 7). (G) Measurements of intra-hepatic TG levels of WT + DMSO and WT + mg149 groups (n = 6). (H) Measurements of serum cholesterol levels of WT + DMSO and WT + mg149 groups (n = 7). (I) Measurements of intra-hepatic cholesterol levels of WT + DMSO and WT + mg149 groups (n = 7). (J) Measurements of serum alanine levels of WT + DMSO and WT + mg149 groups (n = 7). (K) Measurements of intra-hepatic alanine levels of WT + DMSO and WT + mg149 groups (n = 6–7). (L–M) ALT and AST between WT + DMSO and WT + mg149 groups showed no difference (n = 6–7). (N) Biochemical analysis showed that in mg149-treated WT mice, liver glycogen level was decreased (n = 7). (O) Oil-O staining indicated more lipid deposits in mg149-administrated WT mice. Scale bar: 50 μm. See also Figure S4 F. (P) PAS staining indicated less glycogen in mg149-administrated WT mice. Graph data were presented as mean ± SEM. n represents the number of mouse. ∗p < 0.05, ∗∗p < 0.01 (Student’s t test).

Article Snippet: From 8 weeks, one group was given 1 mg/kg mg149 (CAS:1243583-85-8, Sellck, China) dissolved in DMSO, Tween 80 (CAS:9005-65-6, Sellck), and PEG 300 (CAS: 25322-68-3, Selleck), intraperitoneally every other day, while the other was given DMSO solvent as control.

Techniques: Inhibition, Western Blot, Staining

Journal: iScience

Article Title: Mof plays distinct roles in hepatic lipid metabolism under healthy or non-alcoholic fatty liver conditions

doi: 10.1016/j.isci.2023.108446

Figure Lengend Snippet: Impact of Mof inhibition on DIO hepatic biochemical analysis (A and B) IPGTT and IPITT of untreated DIO mice (n = 6 in DIO+DMSO group and n = 10 in DIO+mg149 group). (C and D) IPGTT and IPITT of DMSO or mg149 administrated DIO mice (n = 6 in DIO+DMSO group and n = 10 in DIO+mg149 group). (E) Western blotting showed that mg149 treatment led to decreased hepatic LC3 and H4K16ac expressions (n = 4). (F)Measurements of serum TG levels of DIO+DMSO and DIO+mg149 groups (n = 6 in DIO+DMSO group and n = 9 in DIO+mg149 group). (G) Measurements of intrahepatic TG levels of DIO+DMSO and DIO+mg149 groups (n = 6 in DIO+DMSO group and n = 9 in DIO+mg149 group). (H) Measurements of serum cholesterol levels of DIO+DMSO and DIO+mg149 groups (n = 6 in DIO+DMSO group and n = 9 in DIO+mg149 group). (I) Measurements of intra-hepatic cholesterol levels of DIO+DMSO and DIO+mg149 groups (n = 6 in DIO+DMSO group and n = 9 in DIO+mg149 group). (J) Measurements of serum alanine levels of DIO+DMSO and DIO+mg149 groups (n = 6 in DIO+DMSO group and n = 8 in DIO+mg149 group). (K) Measurements of intra-hepatic alanine levels of DIO+DMSO and DIO+mg149 groups (n = 6 in DIO+DMSO group and n = 9 in DIO+mg149 group). (L and M) ALT (p = 0.0769) and AST (p = 0.7156) between DIO+DMSO and DIO+mg149 groups showed no difference (n = 6 in DIO+DMSO group and n = 9 in DIO+mg149 group). (N) Glycogen measurement indicated no difference in glycogen storage (n = 6 in DIO+DMSO group and n = 9 in DIO+mg149 group). Graph data were presented as mean ± SEM. n represents the number of mouse. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001 (Student’s t test).

Article Snippet: From 8 weeks, one group was given 1 mg/kg mg149 (CAS:1243583-85-8, Sellck, China) dissolved in DMSO, Tween 80 (CAS:9005-65-6, Sellck), and PEG 300 (CAS: 25322-68-3, Selleck), intraperitoneally every other day, while the other was given DMSO solvent as control.

Techniques: Inhibition, Western Blot

Journal: iScience

Article Title: Mof plays distinct roles in hepatic lipid metabolism under healthy or non-alcoholic fatty liver conditions

doi: 10.1016/j.isci.2023.108446

Figure Lengend Snippet: Impact of Mof inhibition on DIO hepatic morphological analysis (A) HE staining showed smaller lipid droplets in mg149 administrated DIO mice. Scale bar: 50 μm. (B) Oil-O staining showed less lipid deposit mg149 administrated DIO mice. Scale bar: 50 μm. See also Figure S4 G. (C) PAS staining indicated no difference in glycogen storage. Scale bar: 50 μm. (D) Western blotting showed decreased p -AKT and p -mTOR levels (n = 4 in DIO+DMSO group and n = 7 in DIO+mg149 group). (E) IHC staining showed decreased p -AKT levels. Scale bar: 50 μm. (F) IHC staining showed decreased p -mTOR levels. Scale bar: 20 μm. Graph data were presented as mean ± SEM. n represents the number of mouse. ∗∗p < 0.01 (Student’s t test).

Article Snippet: From 8 weeks, one group was given 1 mg/kg mg149 (CAS:1243583-85-8, Sellck, China) dissolved in DMSO, Tween 80 (CAS:9005-65-6, Sellck), and PEG 300 (CAS: 25322-68-3, Selleck), intraperitoneally every other day, while the other was given DMSO solvent as control.

Techniques: Inhibition, Staining, Western Blot, Immunohistochemistry

Journal: iScience

Article Title: Mof plays distinct roles in hepatic lipid metabolism under healthy or non-alcoholic fatty liver conditions

doi: 10.1016/j.isci.2023.108446

Figure Lengend Snippet: Impact of Mof inhibition on the metabonomics of DIO livers (A and B) Volcano plot and heatmap of metabolites with the altered amount between DIO+DMSO mice liver and DIO+mg149 mice liver (n = 6 in DIO+DMSO group and n = 10 in DIO+mg149 group). (C) Correlations of altered metabolites. (D) The table listed the most significant metabolites related with observed phenotype. The variable importance in the projection (VIP) value of each variable in the OPLS-DA model was calculated to indicate its contribution to the classification. Significance was determined using an unpaired Student’s t test. VIP value > 1 and p < 0.05 was considered statistically significant. n represents the number of mouse.

Article Snippet: From 8 weeks, one group was given 1 mg/kg mg149 (CAS:1243583-85-8, Sellck, China) dissolved in DMSO, Tween 80 (CAS:9005-65-6, Sellck), and PEG 300 (CAS: 25322-68-3, Selleck), intraperitoneally every other day, while the other was given DMSO solvent as control.

Techniques: Inhibition